Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 651,039 A→G 7.7% L542P (CTC→CCC)  dsbD‑I ← thiol/disulfide interchange protein 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947651,0390AG 7.7% 148.0 / 2.5 52L542P (CTC→CCC) dsbD‑Ithiol/disulfide interchange protein 1
Reads supporting (aligned to +/- strand):  ref base A (20/28);  new base G (2/2);  total (22/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

ACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTACCAGTCCAG  >  NC_002947/650897‑651184
                                                                                                                                               |                                                                                                                                                 
aCCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGa                                                                                                                                                   <  1:105671/143‑1 (MQ=255)
aCCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGa                                                                                                                                                   >  2:105671/1‑143 (MQ=255)
aCCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCt                                                                                                                                             >  7:204567/1‑149 (MQ=255)
 ccGACTTTGCCGCGCTCGCGCGTCAGTATCTGCTCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCtt                                                                                                                                            <  4:247433/149‑1 (MQ=255)
 ccGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCGGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCtt                                                                                                                                            <  2:345228/149‑1 (MQ=255)
    aCTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCg                                                                                                                                         >  5:233991/1‑149 (MQ=255)
          ccGCGCACGCGCGTCAGAATCTGCGCGAATACACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTc                                                                                                                                   <  3:171768/149‑1 (MQ=255)
          ccGCGCACGCGCGTCAGAATCTGCGCGAATACACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTc                                                                                                                                   >  4:171768/1‑149 (MQ=255)
          ccGCGCACGCGCGGCAGAATCTGCCCGAATTCACCGGCATTTAACTCGCCAATCCCCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGGTGCTc                                                                                                                                   <  4:125629/149‑1 (MQ=255)
               cACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAACCTGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCa                                                                                                                               <  1:98741/149‑1 (MQ=255)
               cACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGa                                                                                                                                                   <  5:189523/128‑1 (MQ=255)
               cACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGa                                                                                                                                                   >  6:189523/1‑128 (MQ=255)
                     cgTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACttt                                                                                                                        <  6:84185/149‑1 (MQ=255)
                       tCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCg                                                                                                                      >  5:17933/1‑149 (MQ=255)
                       tCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCg                                                                                                                      <  6:17933/149‑1 (MQ=255)
                           aaTCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGa                                                                                                                  <  2:138791/149‑1 (MQ=255)
                               tGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCg                                                                                                                      >  5:49235/1‑141 (MQ=255)
                               tGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCg                                                                                                                      <  6:49235/141‑1 (MQ=255)
                                 cgcgAATTCACCGGCATTTATCTCGCCTATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTTGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCg                                                                                                             <  6:152763/148‑1 (MQ=255)
                                 cgcgAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGt                                                                                                               <  6:233991/146‑1 (MQ=255)
                                  gcgAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGaa                                                                                                           >  2:363619/1‑149 (MQ=255)
                                  gcgAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACCTTCGGTGATGTCGaa                                                                                                           >  5:80872/1‑149 (MQ=255)
                                         cACCGGCATTTATCTCGCCAATCACCAGATCAGCGGTAATTTCTCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCgcag                                                                                                     <  5:215376/148‑1 (MQ=255)
                                          aCCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCgcagca                                                                                                   <  2:299707/149‑1 (MQ=255)
                                              gCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGttt                                                                                               >  6:150898/1‑149 (MQ=255)
                                                        cGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGGAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGc                                                                                      >  6:199379/1‑148 (MQ=255)
                                                             aTCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTTGAACCGCAGCAGTTTGaa                                                                                            >  5:200912/1‑137 (MQ=255)
                                                             aTCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTTGAACCGCAGCAGTTTGaa                                                                                            <  6:200912/137‑1 (MQ=255)
                                                                 cccGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCg                                                                                        <  1:238106/137‑1 (MQ=255)
                                                                 cccGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCg                                                                                        >  2:238106/1‑137 (MQ=255)
                                                                      tCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACCTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCAc                                                                       <  6:80872/149‑1 (MQ=255)
                                                                       cAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCAcc                                                                      >  8:8888/1‑149 (MQ=255)
                                                                       cAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCAcc                                                                      >  6:341745/1‑149 (MQ=255)
                                                                            gTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCgg                                                                 <  8:204567/149‑1 (MQ=255)
                                                                                    gCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGa                                                         <  5:150898/149‑1 (MQ=255)
                                                                                        ccGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGGGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCAc                                                     >  5:82833/1‑149 (MQ=255)
                                                                                        ccGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGGGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCAc                                                     >  1:172732/1‑149 (MQ=255)
                                                                                             cGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGGGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCa                                                 <  6:82833/148‑1 (MQ=255)
                                                                                             cGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGGGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCa                                                 <  2:172732/148‑1 (MQ=255)
                                                                                                   aaaGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGt                                                                <  7:329555/127‑1 (MQ=255)
                                                                                                   aaaGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGt                                                                >  8:329555/1‑127 (MQ=255)
                                                                                                    aaGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGAt                                         >  8:169904/1‑149 (MQ=255)
                                                                                                     aGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATc                                        <  8:17269/149‑1 (MQ=255)
                                                                                                           agtagggcaggTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCtt                                   <  5:199379/146‑1 (MQ=255)
                                                                                                               gggcaggTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAg                               <  1:363619/149‑1 (MQ=255)
                                                                                                                ggcaggTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGc                              >  2:215981/1‑149 (MQ=255)
                                                                                                                   aggTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGTGCACCTCATGCTCGATCACCTTGCAGCTGa                           <  1:215981/149‑1 (MQ=255)
                                                                                                                    ggTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGAt                          <  2:148382/149‑1 (MQ=255)
                                                                                                                          ccGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGc                                                                   <  3:294893/102‑1 (MQ=255)
                                                                                                                          ccGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGc                                                                   >  4:294893/1‑102 (MQ=255)
                                                                                                                            gAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGt                  >  6:180708/1‑149 (MQ=255)
                                                                                                                                   tGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTa           >  6:316654/1‑149 (MQ=255)
                                                                                                                                     aTAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTAc          <  5:341745/148‑1 (MQ=255)
                                                                                                                                     aTAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTAc          <  7:8888/148‑1 (MQ=255)
                                                                                                                                     aTAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTAc          <  7:228827/148‑1 (MQ=255)
                                                                                                                                              gAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTACCAGTCcag  >  1:119424/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
ACCGACTTTGCCGCGCACGCGCGTCAGAATCTGCGCGAATTCACCGGCATTTATCTCGCCAATCACCCGATCAGCGGTAATTTCGCTGCCGTTCGCAGCAAAGAA‑CAGCAGGGCAGGTGGGCCGAACAGCTGATAGCGGTCGAGCAGCTTGCGTTGCTCGGCATTACTTTCGGTGATGTCGAACCGCAGCAGTTTGAAGCCGGCCAGCTGTGTTTGCACCTGCGGTGCATTGAGCACCTCATGCTCGATCACCTTGCAGCTGATGCACCAGTCGGCGTACCAGTCCAG  >  NC_002947/650897‑651184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑