Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 655,683 G→C 7.6% G220G (GGC→GGG)  PP_0563 ← two‑component system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947655,6830GC 7.6% 245.1 / 8.8 79G220G (GGC→GGG) PP_0563two‑component system response regulator
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base C (4/2);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01

GCGAAACGGCTTGGTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGC  >  NC_002947/655542‑655831
                                                                                                                                             |                                                                                                                                                    
gCGAAACGGCTTGGTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTg                                                                                                                                                >  3:270417/1‑148 (MQ=255)
        gCTTGGTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGc                                                                                                                                       <  1:117018/149‑1 (MQ=255)
        gCTTGGTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGc                                                                                                                                       <  6:248466/149‑1 (MQ=255)
             gTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCAt                                                                                                                                     >  5:57676/1‑146 (MQ=255)
             gTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCAt                                                                                                                                     <  6:57676/146‑1 (MQ=255)
             gTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTa                                                                                                                                  >  5:90681/1‑149 (MQ=255)
               cAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTa                                                                                                                                  >  7:341805/1‑147 (MQ=255)
                aaaaaGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAg                                                                                                                               >  7:293060/1‑149 (MQ=255)
                   aaGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTc                                                                                                                            <  2:341819/149‑1 (MQ=255)
                    aGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCg                                                                                                                           <  8:341805/149‑1 (MQ=255)
                     gTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGa                                                                                                                          <  6:335233/149‑1 (MQ=255)
                        atcaCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAgg                                                                                                                        <  7:27007/148‑1 (MQ=255)
                         tcaCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCTGCACCGGCCCGGAGCTGGccaag                                                                                                                                       <  6:265499/132‑4 (MQ=255)
                         tcaCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCTGCACCGGCCCGGAGCTGGccaag                                                                                                                                       >  5:265499/1‑129 (MQ=255)
                          caCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgat                                                                                                                      >  6:74848/1‑148 (MQ=255)
                          caCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGga                                                                                                                       <  6:134237/147‑1 (MQ=255)
                          caCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGga                                                                                                                       >  5:134237/1‑147 (MQ=255)
                           aCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgatga                                                                                                                    >  6:38547/1‑149 (MQ=255)
                           aCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgatg                                                                                                                     <  8:124388/148‑1 (MQ=255)
                           aCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgatg                                                                                                                     <  8:119269/148‑1 (MQ=255)
                           aCCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTAGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAgcaag                                                                                                                     >  1:145864/1‑144 (MQ=255)
                            cccccTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCACACAGGAAGATGATCGGCACACTCAAATAACGGTCACTGAGGCGGATTACCTTGGACAGCTACGGGCCGGTGCAGGCCGGCATGTACAGGTAGAGgaaga                                                                                                                    >  2:5144/4‑148 (MQ=255)
                             cGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgatgat                                                                                                                   >  3:321691/1‑148 (MQ=255)
                              gCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCTGGCCGGTGCAGGCCGGCATGTACAGGTCGAGgatgat                                                                                                                   >  5:102617/1‑147 (MQ=255)
                               ccTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAg                                                                                                                <  5:292050/149‑1 (MQ=255)
                                     cTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCggg                                                                                                          >  4:28482/1‑149 (MQ=255)
                                      tCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGc                                                                                                         >  3:253448/1‑149 (MQ=255)
                                             aTCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCgg                                                                                                                                        <  8:290544/111‑1 (MQ=255)
                                              tCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTc                                                                                                 <  4:270417/149‑1 (MQ=255)
                                               ccAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCg                                                                                                >  7:46150/1‑149 (MQ=255)
                                                cAGTTGGTTGTGCAGGTCGTCCTCGGCAGACAGGTTGATGATCGGCACACTCACATAAGGGTCAGTGTGGCGGATTAGCTTGGCCAGCTCCGTGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGtag                                                                                                <  1:5144/148‑3 (MQ=255)
                                                cAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCg                                                                                                <  4:253448/148‑1 (MQ=255)
                                                   ttGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCaa                                                                                            <  6:90681/149‑1 (MQ=255)
                                                                  gtcCTCGGCATACATGTAGATGATCGGCACACTCTCATAACGGTGACTGTGGCGGATTTCCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTg                                                                              <  2:270752/148‑1 (MQ=255)
                                                                  gtcCTCCGCAGACAGCTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGc                                                                             <  2:132404/149‑1 (MQ=255)
                                                                     cTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGACTGGAAGTCGGCAAGCTCGGCCATGGTGCGGa                                                                          >  6:257728/1‑149 (MQ=255)
                                                                        ggCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCg                                                                       <  8:293060/149‑1 (MQ=255)
                                                                         gCAGACAGGTTGATGATCGGCACTCTCAGATAACTGTCAGTGTGGCGGATTACCTTGGCCAGCTCGGGGCCGGTGCAGGGCTGCATGTAGAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCgg                                                                      <  2:145864/149‑1 (MQ=255)
                                                                            gACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGggtc                                                                   >  7:256950/1‑149 (MQ=255)
                                                                             aCAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGggtcc                                                                  >  3:310966/1‑148 (MQ=255)
                                                                                ggTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGggtcggt                                                                >  5:271416/1‑148 (MQ=255)
                                                                                 gTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAgg                                                                                                                        >  7:240994/1‑91 (MQ=255)
                                                                                 gTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAgg                                                                                                                        <  8:240994/91‑1 (MQ=255)
                                                                                 gTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGggtcggt                                                                >  2:89763/1‑147 (MQ=255)
                                                                                 gTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACACGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGggtcggt                                                                >  1:280274/1‑147 (MQ=255)
                                                                                  tAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAg                                                             >  6:27378/1‑149 (MQ=255)
                                                                                   aGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAgg                                                            >  7:245392/1‑149 (MQ=255)
                                                                                   aGATGATCGGCACAATCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGATCCGCGCCGGTGCAGGCCGGCATGTACAGCTCCAGGATGATCAGGTCGGCCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAgg                                                            >  5:169811/1‑149 (MQ=255)
                                                                                    gatgatCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGc                                                           >  8:57279/1‑149 (MQ=255)
                                                                                            gCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCTGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGt                                                    >  6:277946/1‑148 (MQ=255)
                                                                                             cacaCTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGa                                                  <  8:46150/149‑1 (MQ=255)
                                                                                             cacaCTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGa                                                  <  5:27378/149‑1 (MQ=255)
                                                                                                      aTAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGAt                                                                         <  6:326564/117‑1 (MQ=255)
                                                                                                      aTAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGAt                                                                         >  5:326564/1‑117 (MQ=255)
                                                                                                        aaCGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATgccggcg                                       >  4:15077/1‑149 (MQ=255)
                                                                                                           ggTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCggg                                                                     >  6:301393/1‑116 (MQ=255)
                                                                                                           ggTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCggg                                                                     <  5:301393/116‑1 (MQ=255)
                                                                                                           ggTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATgccggcgccg                                    >  7:115189/1‑149 (MQ=255)
                                                                                                               aCTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGTGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCa                                <  2:280274/149‑1 (MQ=255)
                                                                                                               aCTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAg                                                 >  5:136077/1‑132 (MQ=255)
                                                                                                               aCTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAg                                                 <  6:136077/132‑1 (MQ=255)
                                                                                                               aCTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATgccggcgccggc                                  >  1:49095/1‑147 (MQ=255)
                                                                                                                    ggCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTaca                           <  1:89763/149‑1 (MQ=255)
                                                                                                                     gCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTacac                          <  2:49095/149‑1 (MQ=255)
                                                                                                                      cGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTacac                          <  5:38547/148‑1 (MQ=255)
                                                                                                                       ggATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGc                        >  8:184634/1‑149 (MQ=255)
                                                                                                                         aTTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTc                      <  5:74848/149‑1 (MQ=255)
                                                                                                                          ttACCTTGGCCAGCTCTGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCg                     <  5:277946/149‑1 (MQ=255)
                                                                                                                            atgccggCCTGCACCGGCCCGGAGCTGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGgt                   <  5:195609/144‑1 (MQ=255)
                                                                                                                            atgccggCCTGCACCGGCCCGGAGCTGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGgt                   >  6:195609/6‑149 (MQ=255)
                                                                                                                             ccTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGgt                   <  8:245392/148‑1 (MQ=255)
                                                                                                                              gccggCCTGCACCGGCCCGGAGCTGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGgtg                  >  6:73358/4‑148 (MQ=255)
                                                                                                                                cggCCTGCACCGGCCCGGAGCTGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTa                >  1:71572/2‑148 (MQ=255)
                                                                                                                                     aGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGc          <  8:256950/149‑1 (MQ=255)
                                                                                                                                       cTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACTCTCGGTGTACATGGCCt        <  4:310966/149‑1 (MQ=255)
                                                                                                                                       cTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCt        <  7:319559/149‑1 (MQ=255)
                                                                                                                                       cTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCt        >  8:319559/1‑149 (MQ=255)
                                                                                                                                         ccGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCAAGTACACGCTCgg                    <  2:34276/135‑1 (MQ=255)
                                                                                                                                         ccGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCAAGTACACGCTCgg                    >  1:34276/1‑135 (MQ=255)
                                                                                                                                             gCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTgc  <  7:57279/149‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                                    
GCGAAACGGCTTGGTCAAAAAGTCATCACCGCCTTCGCTCATGGCATCCAGTTGCTTGTCCAGGTCGTCCTCGGCAGACAGGTAGATGATCGGCACACTCACATAACGGTCACTGTGGCGGATTACCTTGGCCAGCTCCGGGCCGGTGCAGGCCGGCATGTACAGGTCGAGGATGATCAGGTCGGGCTGGAAGTCGGCAAGCTCGGCCATGGTGCGGATCGGGTCGGTCAGGCTGCGGGTGAGCATGCCGGCGCCGGCCAGTACACGCTCGGTGTACATGGCCTGTGTGC  >  NC_002947/655542‑655831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑