Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 660,885 T→C 14.3% intergenic (+17/‑150) speA → / → PP_0568 biosynthetic arginine decarboxylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947660,8850TC14.3% 152.9 / 15.6 63intergenic (+17/‑150)speA/PP_0568biosynthetic arginine decarboxylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (24/30);  new base C (5/4);  total (29/34)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CCGGAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAG  >  NC_002947/660743‑661028
                                                                                                                                              |                                                                                                                                               
ccGGAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCaaaa                                                                                                                                           <  5:305342/149‑1 (MQ=255)
 cGGAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAg                                                                                                                                          >  2:233037/1‑149 (MQ=255)
  ggAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCTCTCCTCAAAAAGc                                                                                                                                         >  2:155397/1‑149 (MQ=255)
  ggAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGc                                                                                                                                         >  5:294674/1‑149 (MQ=255)
  ggAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGc                                                                                                                                         >  2:326912/1‑149 (MQ=255)
  ggAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCCACAAAAgg                                                                                                                                         >  5:254533/1‑148 (MQ=255)
   gAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGcc                                                                                                                                        >  7:83484/1‑149 (MQ=255)
   gAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGcc                                                                                                                                        >  6:23878/1‑149 (MQ=255)
                cGCATTACCTCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCtttt                                                                                                                           <  8:83484/149‑1 (MQ=255)
                 gCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtg                                                                                                                          <  5:23878/149‑1 (MQ=255)
                 gCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtg                                                                                                                          >  7:296263/1‑149 (MQ=255)
                  cATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtgt                                                                                                                         <  6:236327/149‑1 (MQ=255)
                   aTTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtgtg                                                                                                                        >  8:64434/1‑149 (MQ=255)
                    ttACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTgg                                                                                                                       <  4:228317/149‑1 (MQ=255)
                       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTTGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAg                                                                                                                    <  1:155397/149‑1 (MQ=255)
                       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGa                                                                                                                     <  1:233037/148‑1 (MQ=255)
                       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGa                                                                                                                     <  1:199230/148‑1 (MQ=255)
                        cgcgACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGc                                                                                                                   >  1:99570/1‑149 (MQ=255)
                          cgACAAGGTTGACAGCGCCAAGAGAACTGCGCGTCAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGTTCTTCGTACCTGTAGTCGTAATTGACGGGGACGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCgg                                                                                                                 <  7:128844/149‑1 (MQ=25)
                              aaGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGGCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCtt                                                                                                             <  4:323190/149‑1 (MQ=255)
                                ggTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCAGTGCTGCCTTTTGTGGGAGCGGccaagc                                                                                                           >  2:308936/1‑145 (MQ=255)
                                 gTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCg                                                                                                          >  6:160728/1‑149 (MQ=255)
                                     ccAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                      <  1:46602/149‑1 (MQ=255)
                                       aGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                    <  6:24518/149‑1 (MQ=255)
                                       aGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                    <  6:29794/149‑1 (MQ=255)
                                             aaGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAg                                                                                              <  8:100681/149‑1 (MQ=255)
                                               gATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAggg                                                                                            >  5:98381/1‑149 (MQ=255)
                                                    cTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAg                                                                                       <  6:294674/149‑1 (MQ=255)
                                                        gcgTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCt                                                                                   <  7:64434/149‑1 (MQ=255)
                                                         cgTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTGGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCt                                                                                   >  5:311329/1‑148 (MQ=255)
                                                           tGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCAc                                                                                >  4:28493/1‑149 (MQ=255)
                                                                          tCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCg                                                                  <  8:296263/148‑1 (MQ=255)
                                                                              gATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTaaa                                                             >  7:78476/1‑149 (MQ=255)
                                                                              gATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTaa                                                              <  1:1452/148‑1 (MQ=255)
                                                                                  ccTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAatat                                                          <  6:311329/148‑1 (MQ=255)
                                                                                    ttGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCg                                                       >  3:344143/1‑149 (MQ=255)
                                                                                      gcgcCTGGGCTTGACCCGGTCTTCGTACGTGTGGTCGTTATTGTCGGGGACGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCgg                                                      <  1:308936/148‑1 (MQ=255)
                                                                                      gcgcCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCgg                                                      <  3:28493/148‑1 (MQ=255)
                                                                                        gcCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGccc                                                   >  3:267547/1‑149 (MQ=255)
                                                                                         ccTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGt                                                                          >  1:33048/1‑125 (MQ=255)
                                                                                         ccTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGt                                                                          <  2:33048/125‑1 (MQ=255)
                                                                                          cTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTg                                                 >  5:132812/1‑149 (MQ=255)
                                                                                             ggCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTgg                                                <  8:78476/147‑1 (MQ=255)
                                                                                                   aCCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCg                                         <  6:132812/148‑1 (MQ=255)
                                                                                                    cccGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCa                                       >  3:270325/1‑149 (MQ=255)
                                                                                                        gTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCaa                                   <  2:99570/149‑1 (MQ=255)
                                                                                                              gTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATg                                 <  8:115320/145‑1 (MQ=255)
                                                                                                              gTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATg                                 >  7:115320/1‑145 (MQ=255)
                                                                                                                  cTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTAcc                         >  1:238991/1‑149 (MQ=255)
                                                                                                                       gtcgtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGACGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCCACGCACCAATTCCGCTTccgggg                     >  7:234412/1‑144 (MQ=255)
                                                                                                                          gtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATg                 <  2:238991/149‑1 (MQ=255)
                                                                                                                              ttGACGGGGCCGCTCCTGCAAAAGCCTGTGCTTGCTTTTGTGGGTGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAAt             <  6:98381/149‑1 (MQ=255)
                                                                                                                               acgcaaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAAt             <  7:37405/142‑1 (MQ=25)
                                                                                                                                  cGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTc         <  8:167396/149‑1 (MQ=255)
                                                                                                                                   aaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                      <  3:139687/133‑1 (MQ=25)
                                                                                                                                   aaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                      >  4:139687/3‑135 (MQ=25)
                                                                                                                                     ggCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCaaa      <  5:160728/149‑1 (MQ=255)
                                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCCCCAATGCCGCTACCTTTGAATGCAATGTTCAAag     >  3:305204/1‑149 (MQ=37)
                                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCt                        >  7:246258/1‑130 (MQ=25)
                                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCt                        <  8:246258/130‑1 (MQ=25)
                                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAag     >  5:244220/1‑149 (MQ=37)
                                                                                                                                       ccGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAagg    <  4:267547/149‑1 (MQ=255)
                                                                                                                                         gCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAaggag  >  7:171278/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                               
CCGGAAGAGTTGATGACGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAG  >  NC_002947/660743‑661028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑