Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 660,902 C→G 15.4% intergenic (+34/‑133) speA → / → PP_0568 biosynthetic arginine decarboxylase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947660,9020CG15.4% 145.4 / 15.2 57intergenic (+34/‑133)speA/PP_0568biosynthetic arginine decarboxylase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/30);  new base G (4/5);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

CGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGG  >  NC_002947/660759‑661048
                                                                                                                                               |                                                                                                                                                  
cGCATTACCTCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCtttt                                                                                                                                               <  8:83484/149‑1 (MQ=255)
 gCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtg                                                                                                                                              >  7:296263/1‑149 (MQ=255)
 gCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtg                                                                                                                                              <  5:23878/149‑1 (MQ=255)
  cATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtgt                                                                                                                                             <  6:236327/149‑1 (MQ=255)
   aTTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTtgtg                                                                                                                                            >  8:64434/1‑149 (MQ=255)
    ttACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTgg                                                                                                                                           <  4:228317/149‑1 (MQ=255)
       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTTGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAg                                                                                                                                        <  1:155397/149‑1 (MQ=255)
       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGa                                                                                                                                         <  1:199230/148‑1 (MQ=255)
       ccGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGa                                                                                                                                         <  1:233037/148‑1 (MQ=255)
        cgcgACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGc                                                                                                                                       >  1:99570/1‑149 (MQ=255)
          cgACAAGGTTGACAGCGCCAAGAGAACTGCGCGTCAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGTTCTTCGTACCTGTAGTCGTAATTGACGGGGACGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCgg                                                                                                                                     <  7:128844/149‑1 (MQ=25)
              aaGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGGCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCtt                                                                                                                                 <  4:323190/149‑1 (MQ=255)
                ggTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCAGTGCTGCCTTTTGTGGGAGCGGccaagc                                                                                                                               >  2:308936/1‑145 (MQ=255)
                 gTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCg                                                                                                                              >  6:160728/1‑149 (MQ=255)
                     ccAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTcgc                                                                                                                          <  1:46602/149‑1 (MQ=255)
                       aGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                                        <  6:29794/149‑1 (MQ=255)
                       aGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGa                                                                                                                        <  6:24518/149‑1 (MQ=255)
                             aaGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAg                                                                                                                  <  8:100681/149‑1 (MQ=255)
                               gATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAggg                                                                                                                >  5:98381/1‑149 (MQ=255)
                                    cTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAg                                                                                                           <  6:294674/149‑1 (MQ=255)
                                        gcgTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCt                                                                                                       <  7:64434/149‑1 (MQ=255)
                                         cgTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTGGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCt                                                                                                       >  5:311329/1‑148 (MQ=255)
                                           tGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCAc                                                                                                    >  4:28493/1‑149 (MQ=255)
                                                          tCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCg                                                                                      <  8:296263/148‑1 (MQ=255)
                                                              gATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTaaa                                                                                 >  7:78476/1‑149 (MQ=255)
                                                              gATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTaa                                                                                  <  1:1452/148‑1 (MQ=255)
                                                                  ccTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAatat                                                                              <  6:311329/148‑1 (MQ=255)
                                                                    ttGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCg                                                                           >  3:344143/1‑149 (MQ=255)
                                                                      gcgcCTGGGCTTGACCCGGTCTTCGTACGTGTGGTCGTTATTGTCGGGGACGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCgg                                                                          <  1:308936/148‑1 (MQ=255)
                                                                      gcgcCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCgg                                                                          <  3:28493/148‑1 (MQ=255)
                                                                        gcCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGccc                                                                       >  3:267547/1‑149 (MQ=255)
                                                                         ccTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGt                                                                                              >  1:33048/1‑125 (MQ=255)
                                                                         ccTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGt                                                                                              <  2:33048/125‑1 (MQ=255)
                                                                          cTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTg                                                                     >  5:132812/1‑149 (MQ=255)
                                                                             ggCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTgg                                                                    <  8:78476/147‑1 (MQ=255)
                                                                                   aCCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCg                                                             <  6:132812/148‑1 (MQ=255)
                                                                                    cccGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCa                                                           >  3:270325/1‑149 (MQ=255)
                                                                                        gTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCaa                                                       <  2:99570/149‑1 (MQ=255)
                                                                                              gTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATg                                                     <  8:115320/145‑1 (MQ=255)
                                                                                              gTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATg                                                     >  7:115320/1‑145 (MQ=255)
                                                                                                  cTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTAcc                                             >  1:238991/1‑149 (MQ=255)
                                                                                                       gtcgtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGACGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCCACGCACCAATTCCGCTTccgggg                                         >  7:234412/1‑144 (MQ=255)
                                                                                                          gtAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATg                                     <  2:238991/149‑1 (MQ=255)
                                                                                                              ttGACGGGGCCGCTCCTGCAAAAGCCTGTGCTTGCTTTTGTGGGTGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAAt                                 <  6:98381/149‑1 (MQ=255)
                                                                                                               acgcaaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAAt                                 <  7:37405/142‑1 (MQ=25)
                                                                                                                  cGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTc                             <  8:167396/149‑1 (MQ=255)
                                                                                                                   aaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                                          <  3:139687/133‑1 (MQ=25)
                                                                                                                   aaggCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCttt                                          >  4:139687/3‑135 (MQ=25)
                                                                                                                     ggCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCaaa                          <  5:160728/149‑1 (MQ=255)
                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCCCCAATGCCGCTACCTTTGAATGCAATGTTCAAag                         >  3:305204/1‑149 (MQ=37)
                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCt                                            <  8:246258/130‑1 (MQ=25)
                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCt                                            >  7:246258/1‑130 (MQ=25)
                                                                                                                      gCCGCTCCCACAAAAGGCAGCACAGGCTTTTGCAGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAag                         >  5:244220/1‑149 (MQ=37)
                                                                                                                       ccGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAagg                        <  4:267547/149‑1 (MQ=255)
                                                                                                                         gCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAaggag                      >  7:171278/1‑149 (MQ=255)
                                                                                                                            ccaacAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGAc                   <  1:303464/145‑1 (MQ=255)
                                                                                                                                 aaaaGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGatat              <  4:344143/149‑1 (MQ=255)
                                                                                                                                             tgcCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAgg  >  3:153679/1‑149 (MQ=255)
                                                                                                                                              gcCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAAt                                      <  1:292630/112‑1 (MQ=255)
                                                                                                                                              gcCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAAt                                      >  2:292630/1‑112 (MQ=255)
                                                                                                                                               ccTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAgg  <  4:270325/147‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CGCATTACCGCGACAAGGTTGCCAGCGCCAAGATCACTGCGCGTGAGCGGACCCAGTATCTGGATGCCTTGCGCCTGGGCTTGACCCGGTCTTCGTACCTGTCGTCGTAATTGACGGGGCCGCTCCTGCAAAAGCCTGTGCTGCCTTTTGTGGGAGCGGCCTTGCGTCGCGATATGAGGGCGAAGCCCTCACCAGCGTCACAACCGGTAAATATCCGGCCCTGGTCCATCGCACCAATGCCGCTACCTTTGAATGCAATGTTCAAAGGAGGACGATATGCAACGTCCAGG  >  NC_002947/660759‑661048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑