Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	58,439	G→C	11.8%	intergenic (‑350/+338)	PP_5424 ← / ← PP_0050	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	58,439	0	G	C	11.8%	152.6 / 10.7	57	intergenic (‑350/+338)	PP_5424/PP_0050	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (27/22);  new base C (4/2);  total (32/25)
Fisher's exact test for biased strand distribution p-value = 6.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-01

GCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAATC  >  NC_002947/58300‑58586                                                                                                                                            |                                                                                                                                                     gccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTg                                                                                                                                              >  5:13761/1‑148 (MQ=255) gccggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTg                                                                                                                                              <  6:13761/148‑1 (MQ=255)   cggccATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAag                                                                                                                                           <  7:16258/149‑1 (MQ=255)           gTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcg                                                                                                                                   >  1:168530/1‑149 (MQ=255)            tCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGAcgg                                                                                                                                  <  2:150470/149‑1 (MQ=255)                  aTCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCa                                                                                                                            >  3:193531/1‑149 (MQ=255)                            gattgaCACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCg                                                                                                                  >  2:167623/1‑149 (MQ=255)                              ttgaCACGCGTGCCGATTTGGATAGAATTGCAATAGAGGGCTGCAACTGCGCATTTTTCGCGCCTGGTTACCACAATGACCACCAGCAATATTGGCAGAAGAGGAAGCTGATTCCTATGAAGAAGTGACTGCGGGCATAGCCAGACGGa                                                                                                                >  6:154172/1‑149 (MQ=255)                                 acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGaa                                                                                                                                            <  6:235327/118‑1 (MQ=255)                                 acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGaa                                                                                                                                            >  5:235327/1‑118 (MQ=255)                                 acacGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTc                                                                                                             >  1:27933/1‑149 (MQ=255)                                   acGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTg                                                                                                           <  8:152214/149‑1 (MQ=255)                                     gcgTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGaa                                                                                                         >  6:273022/1‑149 (MQ=255)                                         gCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCATTTTTGGCAGCAGAGGAAGCTGGTTGCTCTGAAGTAGTGCCGGCGGGCATTGCCAGGCTGATTCTGAAcc‑c                                                                                                     >  2:265937/1‑148 (MQ=255)                                                 ggATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGc                                                                                             <  2:27933/148‑1 (MQ=255)                                                  gATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGc                                                                                             >  5:72980/1‑147 (MQ=255)                                                  gATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCa                                                                                           <  5:144089/149‑1 (MQ=255)                                                      gCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGAc                                                                                        <  5:273022/148‑1 (MQ=255)                                                       ccTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGAc                                                                                        >  8:227244/1‑147 (MQ=255)                                                        cTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgct                                                                                                                                            >  6:250333/1‑93 (MQ=255)                                                        cTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAACCAGCTTCCTCTgct                                                                                                                                            <  5:250333/95‑3 (MQ=255)                                                         ttGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAg                                                                                     >  6:274644/1‑148 (MQ=255)                                                         ttGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAg                                                                                     >  8:87790/1‑148 (MQ=255)                                                           gCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAgg                                                                                    <  2:168530/147‑1 (MQ=255)                                                           gCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAg                                                                                     <  7:87790/146‑1 (MQ=255)                                                                  aGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAg                                                                            >  3:104735/1‑148 (MQ=255)                                                                  aGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAg                                                                            <  5:22532/148‑1 (MQ=255)                                                                       tGCAACTGCGCATTCGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGAtt                                                                                                              <  1:54880/110‑1 (MQ=255)                                                                       tGCAACTGCGCATTCGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGAtt                                                                                                              >  2:54880/1‑110 (MQ=255)                                                                           aCTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCg                                                                  <  4:104735/149‑1 (MQ=255)                                                                             tGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAg                                                                                     <  8:281484/128‑1 (MQ=255)                                                                             tGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAg                                                                                     >  7:281484/1‑128 (MQ=255)                                                                                gcATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTgg                                                                                                                                                      <  5:143613/61‑1 (MQ=255)                                                                                gcATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTgg                                                                                                                                                      >  6:143613/1‑61 (MQ=255)                                                                                  aTTTGTCGCGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGCGCATTGCCAGTCGGATTCTGAACC‑AAAGGTCGCCACACGAGGAGCTAAAGGATAGCTCCGACTActc                                                           >  2:90571/1‑149 (MQ=255)                                                                                   tttGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTActcc                                                          <  4:193531/149‑1 (MQ=255)                                                                                       tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCAt                                                      <  6:72980/149‑1 (MQ=255)                                                                                       tCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCAt                                                      >  4:336597/1‑149 (MQ=255)                                                                                                ggTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTAcc                                              <  5:274644/148‑1 (MQ=255)                                                                                                  tACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGa                                            >  5:129162/1‑148 (MQ=255)                                                                                                         aTGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATccc                                                   <  3:340089/134‑1 (MQ=255)                                                                                                         aTGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATccc                                                   >  4:340089/1‑134 (MQ=255)                                                                                                                cccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCgtcgg                             <  3:336597/149‑1 (MQ=255)                                                                                                                 ccGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCCCGCgtcggt                            >  1:263307/1‑149 (MQ=255)                                                                                                                  cGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc                                    >  2:322056/1‑140 (MQ=255)                                                                                                                  cGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCAc                                    <  1:322056/140‑1 (MQ=255)                                                                                                                           tacttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGc                  >  8:173846/6‑149 (MQ=255)                                                                                                                           ggCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGc                  >  8:66877/1‑149 (MQ=255)                                                                                                                             cttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGc                                                                   <  3:221898/95‑1 (MQ=255)                                                                                                                             cttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGc                                                                   >  4:221898/4‑98 (MQ=255)                                                                                                                              ttcagAGGAACCAGCTTCCTCTGAAGTAGTGACGGCGGCCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGAGACTACTCCTCATACCTTACCGAAAAGTCAAGCGTCGGTAGTAAACGGCCCt               >  5:97768/3‑149 (MQ=255)                                                                                                                              agcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACCTGAAGGTAGCCAGACTAGGAGCTAAAGGATAGCTGCGACTACAACGAATCCCTTAACGCAAATTAACGCGGCGGTCGTTAACGGccc                >  1:107067/1‑149 (MQ=255)                                                                                                                              agcagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCt               <  6:129162/149‑1 (MQ=255)                                                                                                                                cagAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCttt             >  4:62937/1‑149 (MQ=255)                                                                                                                                   agGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTc            >  1:235294/1‑147 (MQ=255)                                                                                                                                    ggAAGCTGTTTACTCTGAATTATTGACTGCGGGCATTGCCAGTCGGATTCTGAACC‑CAATGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTATCCAg         <  5:154172/149‑1 (MQ=255)                                                                                                                                      ggtcTGGTTCCTCTGAAGTAGTGAGGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCGCTTTCCAGGc       <  6:249627/146‑1 (MQ=255)                                                                                                                                         cTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCaa    >  8:97535/1‑149 (MQ=255)                                                                                                                                          tGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAAt   >  5:325101/1‑149 (MQ=255)                                                                                                                                            gTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAATc  >  7:165863/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                                     GCCGGCCATCGTCATGCATCAGATCTTGATTGACACGCGTGCCGCTTTGGATAGCCTTGCAATTGAGGGCTGCAACTGCGCATTTGTCGGGCCTGGGTACCAGCATGAGCCCCCGCAATTTTGGCAGCAGAGGAAGCTGGTTCCTCTGAAGTAGTGACGGCGGGCATTGCCAGTCGGATTCTGAACC‑CAAGGTCGCCAGACTAGGAGCTAAAGGATCGCTGCGACTACTCCTCATCCCTTACCGAAAAGTCACGCGTCGGTCGTAAACGGCCCTTTCCAGGCCAATC  >  NC_002947/58300‑58586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑