Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 678,251 C→T 7.2% S256N (AGC→AAC)  PP_0581 ← 3‑oxoacyl‑ACP reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947678,2510CT 7.2% 161.1 / 2.2 55S256N (AGC→AAC) PP_05813‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand):  ref base C (27/24);  new base T (2/2);  total (29/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01

GTCGCGGGTGATACCGGCGTTGTGCACCACGATATCGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCAGTCCTCCACCTGGCTGG  >  NC_002947/678106‑678395
                                                                                                                                                 |                                                                                                                                                
gTCGCGGGTGATACAGGCGTTGTGCACCACGATATCGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTgg                                                                                                                                               >  6:42189/1‑149 (MQ=255)
 tCGCGGGTGATACCGGCGTGGTGCACCACGATATCGATGCCGTCGGGCAGTGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCAAGGACCTCGACGCCCTCGCTgca                                                                                                                                              <  3:19974/149‑3 (MQ=255)
   gcgGGTGATACCGGCGTTGTGCACCACGATATCGATGCCGTCGGGCAGGGCCGAGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCt                                                                                                                                            >  1:242869/1‑149 (MQ=255)
   gcgGGTGACACCGGCGGTGTGCACCACGATAGCGATGCCGGCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGGCGAGGTCCTTGCTGGCCt                                                                                                                                            <  6:327573/149‑1 (MQ=255)
       gTGATACCGGCGGTGTGCACCACGATATCGCTGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGGGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCgg                                                                                                                                        <  6:301459/149‑1 (MQ=255)
             ccGGCGTTGTGCACCACGATATCGATGCCGTCGGGCAGGGACGAGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATc                                                                                                                                  >  5:303382/1‑149 (MQ=255)
                         accacGATATCGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAgcagcagca                                                                                                                       >  4:25060/1‑148 (MQ=255)
                                  tCGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGcc                                                                                                             >  8:204474/1‑149 (MQ=255)
                                   cGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCg                                                                                                            >  7:32309/1‑149 (MQ=255)
                                     aTGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGtc                                                                                                          <  7:204474/149‑1 (MQ=255)
                                             gggCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGcc                                                                                                   >  2:213525/1‑148 (MQ=255)
                                               gCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTg                                                                                                     <  5:44476/144‑1 (MQ=255)
                                               gCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTg                                                                                                     >  6:44476/1‑144 (MQ=255)
                                               gCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAg                                                                                                <  1:213525/149‑1 (MQ=255)
                                               gCAGGGCCGCGAGCAGGTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAg                                                                                                <  8:115626/149‑1 (MQ=255)
                                                     ccGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTcc                                                                                          >  7:138902/1‑149 (MQ=255)
                                                     ccGCGAGCAGTTGCGGTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTcc                                                                                          <  6:303382/149‑1 (MQ=255)
                                                             aGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATggc                                                                                  <  3:25060/149‑1 (MQ=255)
                                                               ttgcgttgcCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTCGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATggcgg                                                                                >  7:268055/1‑149 (MQ=255)
                                                                  cgttgcCGCATCGCTCGCGCATATGTCGAGTGGCAGTGGCTTGCCGCGGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGgcgc                                                                             <  7:268094/149‑1 (MQ=255)
                                                                  cgttgcCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGgcgc                                                                             >  2:303925/1‑149 (MQ=255)
                                                                   gttgcCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGcc                                                                            <  1:220353/149‑1 (MQ=255)
                                                                         gCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATAcc                                                                      >  8:69327/1‑149 (MQ=255)
                                                                              gctcgctcATATGTCCAGTGTCAGTGCCTTGCCGCCGAGTCGTGCAGCTAGGGCGTCGAGGTCCTTGCTGGCCTGCTGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCATTGTCGGCGCCGATACCGCGa                                                                  <  8:268055/148‑1 (MQ=255)
                                                                              gctcgcGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGag                                                                 >  2:315298/1‑149 (MQ=255)
                                                                                      atatGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGccccc                                                         <  7:69327/149‑1 (MQ=255)
                                                                                           tCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGAc                                                    >  6:275353/1‑149 (MQ=255)
                                                                                             cAGGGGCAGTGCCTCGCCGCCGAGGCGGGCAGCGAGGGCGTCGACGCCCCCGCCGGCCTGCGGTACCTCCAGCAGCAGCACCTCGCCGCCGGCTCGTGCCAAGGTTTCCGCCATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGAccc                                                  <  2:242869/149‑2 (MQ=255)
                                                                                              aGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAg                                                 >  3:58549/1‑149 (MQ=255)
                                                                                               gTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAgc                                                <  8:32309/149‑1 (MQ=255)
                                                                                                  gCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATc                                                                                                                                  >  5:175/1‑64 (MQ=255)
                                                                                                  gCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATc                                                                                                                                  <  6:175/64‑1 (MQ=255)
                                                                                                  gCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCCGCCCa                                             >  8:80601/1‑149 (MQ=255)
                                                                                                     gTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCAcgg                                          <  1:303925/149‑1 (MQ=255)
                                                                                                        ccTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCCCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCCTGACCAGCGCAcggcgg                                       >  6:297303/1‑149 (MQ=255)
                                                                                                             ccgccgAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGTAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTCACCAGCGCACGGCGgccgcc                                  >  6:337422/1‑149 (MQ=255)
                                                                                                             ccgccgAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGgccgcc                                  >  6:244341/1‑149 (MQ=255)
                                                                                                                     gCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          >  4:103092/1‑149 (MQ=255)
                                                                                                                       gTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCgg                        <  5:244341/149‑1 (MQ=255)
                                                                                                                          cAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTc                     <  4:58549/149‑1 (MQ=255)
                                                                                                                          cAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTc                     <  8:138902/149‑1 (MQ=255)
                                                                                                                           aGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATACAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCAGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTc                     >  5:230812/1‑148 (MQ=255)
                                                                                                                            gCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCa                   <  5:275353/149‑1 (MQ=255)
                                                                                                                                    gTCGAGGTCCTTGTTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          >  1:169338/1‑134 (MQ=255)
                                                                                                                                    gTCGAGGTCCTTGTTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          >  1:307083/1‑134 (MQ=255)
                                                                                                                                    gTCGAGGTCCTTGTTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          <  2:169338/134‑1 (MQ=255)
                                                                                                                                    gTCGAGGTCCTTGTTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          <  2:307083/134‑1 (MQ=255)
                                                                                                                                     tCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCAGTCCTCCAc          >  6:269449/1‑149 (MQ=255)
                                                                                                                                       gAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCg                         >  3:158314/1‑132 (MQ=255)
                                                                                                                                       gAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCg                         <  4:158314/132‑1 (MQ=255)
                                                                                                                                         ggTCCTTGCTCGCCTGCGGTACAACCAGCAGCAGCACATCGGCGCCCTCTCGTGCCAAGCTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGAGGCCGCCTAGTGGGCGGGTCCAGTCCTCCACctgg      >  3:345686/1‑149 (MQ=255)
                                                                                                                                          gTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCAGTCCTCCACctggc     >  4:336641/1‑149 (MQ=255)
                                                                                                                                             cTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGTAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCAGTCCTCCACctggctgg  <  5:337422/149‑1 (MQ=255)
                                                                                                                                             cTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          >  3:280285/1‑125 (MQ=255)
                                                                                                                                             cTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGc                          <  4:280285/125‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCGCGGGTGATACCGGCGTTGTGCACCACGATATCGATGCCGTCGGGCAGGGCCGCGAGCAGTTGCGTTGCCGCATCGCTCGCGCATATGTCCAGTGGCAGTGCCTTGCCGCCGAGGCGTGCAGCGAGGGCGTCGAGGTCCTTGCTGGCCTGCGGTACATCCAGCAGCAGCACATCGGCGCCGTCTCGTGCCAAGGTTTCCGCAATGGCGGCGCCGATACCGCGAGCAGCCCCCGTGACCAGCGCACGGCGGCCGCCTAGTGGGCGGGTCCAGTCCTCCACCTGGCTGG  >  NC_002947/678106‑678395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑