Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	790,154	A→T	10.4%	intergenic (+42/+18)	gdhA → / ← PP_0676	NADP‑dependent glutamate dehydrogenase/transcription elongation factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	790,154	0	A	T	10.4%	104.9 / 2.4	38	intergenic (+42/+18)	gdhA/PP_0676	NADP‑dependent glutamate dehydrogenase/transcription elongation factor
Reads supporting (aligned to +/- strand):  ref base A (13/21);  new base T (2/2);  total (15/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGAT  >  NC_002947/790011‑790294                                                                                                                                                |                                                                                                                                             tACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAag                                                                                                                                           >  2:254238/1‑147 (MQ=255)      tGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCa                                                                                                                                              >  1:358098/1‑139 (MQ=255)      tGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCa                                                                                                                                              <  2:358098/139‑1 (MQ=255)         agagGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCTTTGCAGcccatcg                                                                                                                                 <  5:88896/149‑5 (MQ=255)              cTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAACGTGGCCGATGCGATGCTGGCGCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCt                                                                                                                            <  3:354760/149‑1 (MQ=255)                gATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGAAGGGCTGCAAAGCAGCCCCAGCGcccac                                                                                                                           >  7:80774/1‑146 (MQ=255)                 aTGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCaa                                                                                                                         <  3:66327/149‑1 (MQ=255)                      cGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTc                                                                                                                    <  3:344904/149‑1 (MQ=255)                            aaCTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGcc                                                                                                                 >  3:128091/1‑146 (MQ=255)                                aCGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAAtc                                                                                                          >  1:262208/1‑149 (MQ=255)                                  gTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGCCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCaa                                                                                                                         >  6:31032/1‑132 (MQ=255)                                  gTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGCCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCaa                                                                                                                         <  5:31032/132‑1 (MQ=255)                                     aaGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCaa                                                                                                      <  2:262208/148‑1 (MQ=255)                                          ggCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACct                                                                                                <  3:204227/149‑1 (MQ=255)                                               aCATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGaa                                                                                                            <  7:200748/132‑1 (MQ=255)                                               aCATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGaa                                                                                                            >  8:200748/1‑132 (MQ=255)                                                  tCGGGGGCTTCGTGAATGTGGCCTATCCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGTCCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCAcc                                                                                        <  4:350226/149‑1 (MQ=255)                                                    gcgGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTg                                                                                      >  4:174498/1‑149 (MQ=255)                                                          ttCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAac                                                                                                     >  8:149261/1‑128 (MQ=255)                                                          ttCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAac                                                                                                     <  7:149261/128‑1 (MQ=255)                                                                     ggCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgcc                                                                     <  1:254238/149‑1 (MQ=255)                                                                      gCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgcc                                                                     <  3:196503/148‑1 (MQ=255)                                                                      gCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgcc                                                                     <  6:132609/148‑1 (MQ=255)                                                                       ccGATGCGATGCTGGCTCAGGGCGTTGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgccgc                                                                   <  6:74411/149‑1 (MQ=255)                                                                        cgatgcgatgCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgccgc                                                                   <  3:227551/148‑1 (MQ=255)                                                                                tgcTGGCTCTGGGCTTGTTCTTATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATTGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGaccacc                                                          <  8:80774/149‑1 (MQ=255)                                                                                         aGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCAc                                                                                         <  2:235105/109‑1 (MQ=255)                                                                                         aGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTc                                                        <  4:124736/142‑1 (MQ=255)                                                                                         aGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTc                                                        >  3:124736/1‑142 (MQ=255)                                                                                                       tctgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTccccc                                                                               <  2:54150/105‑1 (MQ=255)                                                                                                       tctgaGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTccccc                                                                               >  1:54150/1‑105 (MQ=255)                                                                                                          gagaCGGTAGGGGGCCGCGTTGGGTCGCGATGGGCTGCTAAGCAGCCCCAGGGCCCTCTACCCTCGCCCAGAATCTCAACCTCCTCTGTCACCGGTTCGCCCGCCGGCCGGCGGCAGCCCCCCGCCACCCCCGgccccgcccccgcgc                                 >  6:119174/1‑143 (MQ=255)                                                                                                                                cGTCGCGATGGGCTGCAAAGCAGCCCCAGGG‑CCTCAACCCTCGCCCAGAATCTCAACCACCTCTATc                                                                                           >  2:295271/1‑67 (MQ=255)                                                                                                                                    gcgATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGt      <  3:174498/149‑1 (MQ=255)                                                                                                                                     cgATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGt                                                                                     <  4:155498/69‑1 (MQ=255)                                                                                                                                     cgATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGt                                                                                     >  3:155498/1‑69 (MQ=255)                                                                                                                                      gATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgccgc                                                                   >  7:208353/1‑86 (MQ=255)                                                                                                                                      gATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGgccgccgc                                                                   <  8:208353/86‑1 (MQ=255)                                                                                                                                       tggggCTGCTTTGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCCGCCCAGCCCCCACCAAAGCCCGCCCCCATGGCGCACCCCacgtg    >  8:112312/3‑144 (MQ=255)                                                                                                                                         gggCTGCTTTGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGAt  <  7:112312/148‑1 (MQ=255)                                                                                                                                                |                                                                                                                                             TACGGTGAAGAGGCTGATGGCCGGATCAACTACGTCAAGGGGGCGAACATCGCGGGCTTCGTGAAAGTGGCCGATGCGATGCTGGCTCAGGGCGTGGTCTGATCTGAGACCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAGACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGAT  >  NC_002947/790011‑790294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑