Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 805,137 G→T 5.9% S731S (TCC→TCA) ‡ PP_0693 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947805,1370GT 5.9% 252.3 / 3.5 78S731S (TCC→TCA) ‡PP_0693hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (39/34);  new base T (2/3);  total (41/37)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01

CTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGTA  >  NC_002947/804990‑805284
                                                                                                                                                   |                                                                                                                                                   
cTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTg                                                                                                                                                     <  3:7193/148‑1 (MQ=255)
cTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTg                                                                                                                                                     <  4:58534/148‑1 (MQ=255)
    ttgtCCTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAAc                                                                                                                                                <  5:92544/149‑1 (MQ=255)
         cTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTTCCAACCTgg                                                                                                                                            <  5:158153/148‑1 (MQ=255)
         cTGACCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGgc                                                                                                                                           <  8:228884/149‑1 (MQ=255)
            aTCGGCACTCTGGGTCAGGTCGGAGACCGACAGGTTTTCCCCTTGTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGATACGTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTGGGAACCCTGgc                                                                                                                                           >  7:228884/1‑146 (MQ=255)
             tCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCtt                                                                                                                                       <  3:71761/149‑1 (MQ=255)
             tCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCtt                                                                                                                                       <  4:250443/149‑1 (MQ=255)
               ggCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttg                                                                                                                                      <  3:145944/148‑1 (MQ=255)
                    tctGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtc                                                                                                                                >  6:40359/1‑149 (MQ=255)
                    tctGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtc                                                                                                                                >  5:164379/1‑149 (MQ=255)
                    tctGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtc                                                                                                                                >  2:290496/1‑149 (MQ=255)
                         gtgaGGTCGTAGACCGACAGGGTTTGGGCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTGCTTGTTCGTCGCCAGTACCACGCGGAAGCTTTGCAGGTTTGAAACCTGTCGCTTGTTGATCTcggg                                                                                                                           <  2:94252/146‑3 (MQ=255)
                         gTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTcgcg                                                                                                                           >  8:252947/1‑149 (MQ=255)
                          tCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtctc                                                                                                                              <  3:155296/145‑1 (MQ=255)
                          tCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGAtctc                                                                                                                              >  4:155296/1‑145 (MQ=255)
                            aGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGtt                                                                                                                        <  5:40359/149‑1 (MQ=255)
                                   aGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAgg                                                                                                                 >  2:153871/1‑149 (MQ=255)
                                   aGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAgg                                                                                                                 <  6:164379/149‑1 (MQ=255)
                                    tacagACAGGGTTTCGCCTTCATCGTACTGGCCGGCACTGTGGATAATCTTGTCGATGTGCTTGAGAGATTCCTTGTTCGGCGCCAGTACAACGCGGAAGCTTTCAAGGATGTAAACATGGCGCTTGTTGATCTCGCGGTTGAACAgg                                                                                                                 >  4:134208/5‑148 (MQ=255)
                                         aCAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGc                                                                                                           >  6:224000/1‑149 (MQ=255)
                                               tttCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGc                                                                                                     >  5:74031/1‑149 (MQ=255)
                                                    ccTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAg                                                                                                <  4:194423/149‑1 (MQ=255)
                                                        cttcGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTg                                                                                            >  3:38963/1‑149 (MQ=255)
                                                             tACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCg                                                                                         >  7:286046/1‑147 (MQ=255)
                                                              aCTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAg                                                                                      >  6:56358/1‑149 (MQ=255)
                                                               cTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAg                                                                                      <  5:358175/148‑1 (MQ=255)
                                                               cTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAg                                                                                      <  5:224000/148‑1 (MQ=255)
                                                                          tgtgGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGAGCCAGTACCACGCGGAAGCTTTACAGGTTGGAAACCTGGCGCTTGTAGATCTCGCGGTTGAACAGGGCAAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATg                                                                          >  2:72781/1‑149 (MQ=255)
                                                                           gtgGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCg                                                                                                  >  6:25983/1‑124 (MQ=255)
                                                                           gtgGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCg                                                                                                  <  5:25983/124‑1 (MQ=255)
                                                                              gATAATCTGGTGGATGTGGTTGAGCGCTTCCTTGTTCGGAGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAATCCTGGCGGTTGTTGATCTCGCGGTTGAACAGGGGCAGCTGGTGCTCGAGGCTGTCGt                                                                                        <  8:68027/131‑1 (MQ=255)
                                                                              gATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGt                                                                                        >  7:68027/1‑131 (MQ=255)
                                                                              gATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTgc                                                                      >  5:50613/1‑149 (MQ=255)
                                                                                     tGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCg                                                                                                  <  2:51205/114‑1 (MQ=255)
                                                                                     tGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCg                                                                                                  >  1:51205/1‑114 (MQ=255)
                                                                                       gTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAgg                                                                 <  5:56358/145‑1 (MQ=255)
                                                                                       gTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTc                                                             >  3:83147/1‑149 (MQ=255)
                                                                                        tCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCg                                                            <  1:153871/149‑1 (MQ=255)
                                                                                        tCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCg                                                            <  7:252947/149‑1 (MQ=255)
                                                                                         cGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTc                                                                                          <  6:336706/118‑1 (MQ=255)
                                                                                         cGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAgg                                                                 <  7:43955/143‑1 (MQ=255)
                                                                                         cGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAgg                                                                 >  8:43955/1‑143 (MQ=255)
                                                                                         cGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCgg                                                           <  1:290496/149‑1 (MQ=255)
                                                                                               gCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCggg                                                          <  6:34502/144‑1 (MQ=255)
                                                                                               gCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCggg                                                          >  5:34502/1‑144 (MQ=255)
                                                                                                 ttGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTc                                                   >  5:66707/1‑149 (MQ=255)
                                                                                                  tGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttg                                                                                                                                      >  8:74804/1‑65 (MQ=255)
                                                                                                  tGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCttg                                                                                                                                      <  7:74804/65‑1 (MQ=255)
                                                                                                   gAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCgg                                                 >  2:229044/1‑149 (MQ=255)
                                                                                                         ttCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCg                                           >  1:340330/1‑149 (MQ=255)
                                                                                                            cTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAg                                        >  6:132635/1‑149 (MQ=255)
                                                                                                             ttGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGc                                                                                                           <  2:327692/81‑1 (MQ=255)
                                                                                                             ttGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGc                                                                                                           >  1:327692/1‑81 (MQ=255)
                                                                                                               gTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCgg                                                           <  6:243099/127‑1 (MQ=255)
                                                                                                                    gcgcCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGt                                <  6:74031/149‑1 (MQ=255)
                                                                                                                    gcgcCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGt                                >  3:152803/1‑149 (MQ=255)
                                                                                                                      gcCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGc                              <  1:229044/149‑1 (MQ=255)
                                                                                                                         aGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCtt                           >  8:123995/1‑149 (MQ=255)
                                                                                                                         aGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCtt                           >  6:246027/1‑149 (MQ=255)
                                                                                                                          ttcgcccgcgGAAGCTTTCCCGGGTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                          <  6:250977/143‑1 (MQ=255)
                                                                                                                          gTACCACGCGGAAGCTTTCCAGGTTGGAAAGCTGGCGCTTGTTGATCTCGCGGTTGAAGAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                          <  8:286046/149‑1 (MQ=255)
                                                                                                                            aCCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                          <  4:332566/147‑1 (MQ=255)
                                                                                                                            aCCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                          >  3:332566/1‑147 (MQ=255)
                                                                                                                               aCGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGGCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTg                          >  5:250977/1‑144 (MQ=255)
                                                                                                                                   ggAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTc                 >  4:258930/1‑149 (MQ=255)
                                                                                                                                     aaGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCTGTTGAACAGGGCCAGCTGGTGCTCGAGGCGGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTTCCTTGGCCAAGGTCAg               <  1:72781/149‑1 (MQ=255)
                                                                                                                                     aaGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAg               <  6:50613/149‑1 (MQ=255)
                                                                                                                                     aaGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAg               >  6:83171/1‑149 (MQ=255)
                                                                                                                                     aaGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAg               >  8:293070/1‑149 (MQ=255)
                                                                                                                                      aGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAgg              >  5:197480/1‑149 (MQ=255)
                                                                                                                                        cTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGc            >  2:211605/1‑149 (MQ=255)
                                                                                                                                         gcgccAGGTTTCCAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCa                >  6:37186/4‑144 (MQ=255)
                                                                                                                                         gcgccAGGTTTCCAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCa                <  5:37186/141‑1 (MQ=255)
                                                                                                                                           tCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtc         >  1:155392/1‑149 (MQ=255)
                                                                                                                                           gccAGGTTTCCAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtc         >  2:247853/2‑149 (MQ=255)
                                                                                                                                             cAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAggg             <  4:223694/143‑1 (MQ=255)
                                                                                                                                             cAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAggg             >  3:223694/1‑143 (MQ=255)
                                                                                                                                               ggTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcgtcg     <  5:132635/149‑1 (MQ=255)
                                                                                                                                               ggTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTtcgtcg     <  4:38963/149‑1 (MQ=255)
                                                                                                                                                   ggAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCTCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGTa  <  5:83171/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTTGTTGTCCTGATCGGCACTCTGGGTCAGGTCGAAGACCGACAGGGTTTCGCCTTCTTCGTACTGGCCGGCACTGTGGATAATCTGGTCGATGTGCTTGAGCGCTTCCTTGTTCGGCGCCAGTACCACGCGGAAGCTTTCCAGGTTGGAAACCTGGCGCTTGTTGATCTCGCGGTTGAACAGGGCCAGCTGGTGCTCGAGGCTGTCGTAGTCGCTGCGGATGTTGCGCAGGGTTCGGGCGATGTCGGTGACCGCAGCGCGGCGTGCCTTGGCCAAGGTCAGGGCTTCGTCGGTA  >  NC_002947/804990‑805284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑