Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 874,551 T→C 5.3% Y44H (TAC→CAC)  PP_0759 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947874,5510TC 5.3% 224.8 / 2.0 75Y44H (TAC→CAC) PP_0759hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (38/33);  new base C (2/2);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCTGAT  >  NC_002947/874408‑874699
                                                                                                                                               |                                                                                                                                                    
cTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCAGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATg                                                                                                                                                 >  5:309129/1‑149 (MQ=255)
     aTACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATg                                                                                                                                                 >  4:230302/1‑144 (MQ=255)
     aTACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATg                                                                                                                                                 <  3:230302/144‑1 (MQ=255)
      tACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGc                                                                                                                                           <  3:327299/149‑1 (MQ=255)
       aCGGCGCATGAGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCa                                                                                                                                          >  6:110575/1‑149 (MQ=255)
            gcATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCAGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGctc                                                                                                                                     <  6:309129/149‑1 (MQ=255)
              aTGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCtctc                                                                                                                                   >  1:110730/1‑149 (MQ=255)
              aTGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGAGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCtctc                                                                                                                                   <  1:296587/149‑1 (MQ=255)
                  ggATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCAt                                                                                                                                         <  7:290374/139‑1 (MQ=255)
                  ggATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCAt                                                                                                                                         >  8:290374/1‑139 (MQ=255)
                        ggAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAg                                                                                                                         <  5:110575/149‑1 (MQ=255)
                        ggAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAg                                                                                                                         >  3:119112/1‑149 (MQ=255)
                         gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGctct                                                                                                                                    >  1:238790/1‑137 (MQ=255)
                         gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGctct                                                                                                                                    <  2:238790/137‑1 (MQ=255)
                         gAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGATGGCCGGCTGCCAGGCGGTGAATACAACCAGAGGAGGTGCATTTGGCGTCCAACGCCAACAGTACATGTTCAACATGCACTCGACCGATGAg                                                                                                                         >  5:59895/1‑148 (MQ=255)
                          aGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                            >  1:322767/1‑144 (MQ=255)
                          aGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                            <  2:322767/144‑1 (MQ=255)
                          aGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGt                                                                                                                       >  4:2230/1‑149 (MQ=255)
                                 tCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCa                                                                                                                 <  8:168710/148‑1 (MQ=255)
                                   aTGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCTATGAGGTCAACCAGa                                                                                                               <  7:274921/148‑1 (MQ=255)
                                   aTGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                              >  6:143706/1‑149 (MQ=255)
                                    tGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGAc                                                                                                                                <  7:157896/130‑1 (MQ=255)
                                    tGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGAc                                                                                                                                >  8:157896/1‑130 (MQ=255)
                                       gTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGACAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTAc                                                                                                          >  4:298506/1‑149 (MQ=255)
                                              ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGt                                                                                                   >  5:74798/1‑149 (MQ=255)
                                              ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGt                                                                                                   >  1:349268/1‑149 (MQ=255)
                                              ttttGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATAACACCAGCGGCGGTGCTGTTTGCGTCGAACGCCAACAGTACATGTTAAGCAAGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGt                                                                                                   >  1:285768/1‑149 (MQ=255)
                                                   tcatcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                            <  3:179025/119‑1 (MQ=255)
                                                   tcatcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGAt                                                                                                                            >  4:179025/1‑119 (MQ=255)
                                                     atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGa                                                                                                                          >  3:312903/1‑119 (MQ=255)
                                                     atcaGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGa                                                                                                                          <  4:312903/119‑1 (MQ=255)
                                                           ctgctgAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGAc                                                                                      <  3:2230/149‑1 (MQ=255)
                                                                     cTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTg                                                                              >  8:75377/1‑147 (MQ=255)
                                                                     cTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGaa                                                                            <  5:143706/149‑1 (MQ=255)
                                                                      tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGa                                                                                                                                 <  1:126017/95‑1 (MQ=255)
                                                                      tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGa                                                                                                                                 >  2:126017/1‑95 (MQ=255)
                                                                      tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAg                                                                           >  2:175976/1‑149 (MQ=255)
                                                                      tGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAg                                                                           >  6:100476/1‑149 (MQ=255)
                                                                                ctgGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCaa                                                                 <  6:74798/149‑1 (MQ=255)
                                                                                   gCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                               <  3:298506/148‑1 (MQ=255)
                                                                                          gCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATg                                                                                                                           <  1:300895/81‑1 (MQ=255)
                                                                                          gCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATg                                                                                                                           >  2:300895/1‑81 (MQ=255)
                                                                                           ccAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                               <  8:20535/140‑1 (MQ=255)
                                                                                           ccAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                               >  7:20535/1‑140 (MQ=255)
                                                                                           ccAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTc                                                       >  1:319990/1‑148 (MQ=255)
                                                                                             aGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGAc                                                    <  1:175976/149‑1 (MQ=255)
                                                                                               gCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGTATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGAc                                                    >  4:100213/1‑147 (MQ=255)
                                                                                                 ggTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGt                                                <  7:75377/149‑1 (MQ=255)
                                                                                                  gTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                               >  6:224607/1‑149 (MQ=255)
                                                                                                  gTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                               >  6:229796/1‑149 (MQ=255)
                                                                                                   tGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTc                                               >  3:100270/1‑148 (MQ=255)
                                                                                                      aTACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg                                                                                 <  4:151948/111‑1 (MQ=255)
                                                                                                      aTACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTgg                                                                                 >  3:151948/1‑111 (MQ=255)
                                                                                                      aTACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAgc                                           >  7:81588/1‑149 (MQ=255)
                                                                                                          caccaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGcc                                        >  4:323326/1‑148 (MQ=255)
                                                                                                           accaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGa                                      <  5:229796/149‑1 (MQ=255)
                                                                                                           accaGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGa                                      <  5:224607/149‑1 (MQ=255)
                                                                                                                 ggcggTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGTATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCaa                                <  3:100213/149‑1 (MQ=255)
                                                                                                                     gTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgt                            <  3:323326/149‑1 (MQ=255)
                                                                                                                     gTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCgt                            <  4:100270/149‑1 (MQ=255)
                                                                                                                      tacTGTTGGTGTTCGACGCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGgtg                                                            >  8:2214/3‑116 (MQ=255)
                                                                                                                      tacTGTTGGTGTTCGACGCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGgtg                                                            <  7:2214/114‑1 (MQ=255)
                                                                                                                      tacTGTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                               >  8:182594/3‑113 (MQ=255)
                                                                                                                      tacTGTTGGCGTTCGACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAgg                                                               <  7:182594/111‑1 (MQ=255)
                                                                                                                            tGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTAAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGTGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAg                      <  6:59895/148‑1 (MQ=255)
                                                                                                                               cGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCaa                                >  5:170467/1‑135 (MQ=255)
                                                                                                                               cGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCaa                                <  6:170467/135‑1 (MQ=255)
                                                                                                                                gTCGAACGCCAACAGTACATGTTCGGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCAt                 >  4:343306/1‑149 (MQ=255)
                                                                                                                                gTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                              >  8:152618/1‑56 (MQ=255)
                                                                                                                                gTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGAt                                                                                                              <  7:152618/56‑1 (MQ=255)
                                                                                                                                   gAACGCCAACAGTACATGTTCAGCATGCTCTCGAGCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCa                       <  4:233865/140‑1 (MQ=255)
                                                                                                                                   gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCa                       >  3:233865/1‑140 (MQ=255)
                                                                                                                                   gAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGc              >  3:333175/1‑149 (MQ=255)
                                                                                                                                       gCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                                     <  8:17396/122‑1 (MQ=255)
                                                                                                                                       gCCAACAGCACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGAt                                     >  7:17396/1‑122 (MQ=255)
                                                                                                                                               tACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCTGAt  >  1:35954/1‑149 (MQ=255)
                                                                                                                                               tACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCTGAt  >  8:330607/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
CTAGGATACGGCGCATGTGGATAGGGAGTGTTTTCATGCGTAAGTCTTTTGTCATCAGCCTGCTGAGTGCTGGCATCCTGCTGGCCGGCTGCCAGGCGGTGAATACCACCAGCGGCGGTGCTGTTGGCGTCGAACGCCAACAGTACATGTTCAGCATGCTCTCGACCGATGAGGTCAACCAGATGTACGCGCAGTCGTATCAGCAGACCCTGGGTGAAGCATCGAGCAAGGGTGTGCTCGACAAGTCCAGCGCCGATGCCAAGCGTGTGCAGGCCATTGCCAACCGCCTGAT  >  NC_002947/874408‑874699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑