Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	876,015	G→C	6.8%	noncoding (105/142 nt)	PP_mr65 ←	YybP‑YkoY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	876,015	0	G	C	6.8%	186.2 / 3.7	59	noncoding (105/142 nt)	PP_mr65	YybP‑YkoY
Reads supporting (aligned to +/- strand):  ref base G (34/21);  new base C (2/2);  total (36/23)
Fisher's exact test for biased strand distribution p-value = 6.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

ACCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGTTTTTTA  >  NC_002947/875872‑876162                                                                                                                                                |                                                                                                                                                    aCCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTg                                                                                                                                                >  1:160185/1‑149 (MQ=255)       aGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCAtggt                                                                                                                                          <  1:55978/149‑1 (MQ=255)       aGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtgga                                                                                                                                          <  2:39329/149‑2 (MQ=255)       aGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtg                                                                                                                                            >  1:39329/1‑147 (MQ=255)         tGATTCCAGCATCAGGATTACTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCACGCCTGCAtggaga                                                                                                                                        <  2:241695/149‑4 (MQ=255)          gATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTggg                                                                                                                                       >  1:134116/1‑149 (MQ=255)             tCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCg                                                                                                                                    >  7:261667/1‑149 (MQ=255)                 gCATCAGGATTCCTACGGGGGCGGGTCAACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCAcg                                                                                                                                >  7:130438/1‑149 (MQ=255)                  cATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCAcgc                                                                                                                               <  2:134116/149‑1 (MQ=255)                      aGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCa                                                                                                                           <  8:261667/149‑1 (MQ=255)                       ggATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCAt                                                                                                                          >  2:97106/1‑149 (MQ=255)                              tACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTggg                                                                                                                                       >  4:190271/1‑129 (MQ=255)                              tACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTggg                                                                                                                                       <  3:190271/129‑1 (MQ=255)                               aCGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCtgtg                                                                                                                  >  2:591/1‑149 (MQ=255)                                      cGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAg                                                                                                           <  1:591/149‑1 (MQ=255)                                             acacGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCAGTCTATGCGGAACTATCCATGCAGGCGTGCATGCTGGTTCGCACGCGCCATGGTCTGTGGACCAAGTATGtt                                                                                                     >  8:125092/1‑148 (MQ=255)                                                    tATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTa                                                                                             >  5:56826/1‑149 (MQ=255)                                                      tGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGtt                                                                                                     <  7:24089/139‑1 (MQ=255)                                                      tGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGtt                                                                                                     >  8:24089/1‑139 (MQ=255)                                                      tGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGCCGCGTagc                                                                                           >  2:291034/1‑147 (MQ=255)                                                        acacGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCgg                                                                                         >  1:317855/1‑149 (MQ=255)                                                            gTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGc                                                                                       >  3:214250/1‑147 (MQ=255)                                                                 gCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                                <  2:160185/149‑1 (MQ=255)                                                                   cTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTAcc                                                                                           <  4:199646/136‑1 (MQ=255)                                                                   cTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTAcc                                                                                           >  3:199646/1‑136 (MQ=255)                                                                     ccccgccccgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGc                                                                            >  5:275443/1‑149 (MQ=255)                                                                            ccgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                      >  4:70730/1‑148 (MQ=255)                                                                             cgGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGga                                                                    <  4:214250/149‑1 (MQ=255)                                                                               ggTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGgaga                                                                  <  8:130438/149‑1 (MQ=255)                                                                                    ggTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGactact                                                             >  2:344933/1‑149 (MQ=255)                                                                                     gtgtGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTAt                                                                                                        >  4:145092/1‑105 (MQ=255)                                                                                     gtgtGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTAt                                                                                                        <  3:145092/105‑1 (MQ=255)                                                                                        tgCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTccc                                                          <  1:344933/148‑1 (MQ=255)                                                                                         gCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTccctc                                                        >  3:136197/1‑147 (MQ=255)                                                                                                aTAGGTCTTGTCAAACCCTCGATCCGTCTGTGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGgaga                                                                  <  8:21710/132‑1 (MQ=255)                                                                                                aTAGGTCTTGTCAAACCCTCGATCCGTCTGTGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGgaga                                                                  >  7:21710/1‑132 (MQ=255)                                                                                                 tAGGTCTTGTCAAACACTCGATCCGTCTATGCGGATCTCTCAATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGAAGGt                                                >  5:336144/1‑149 (MQ=255)                                                                                                     tCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCg                                             >  8:196900/1‑148 (MQ=255)                                                                                                          tCAAACCCTCGATCCGTCTATGCGGACCTCTCCATCCAGGCGTCCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCt                                       <  6:305034/149‑1 (MQ=255)                                                                                                             aaCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGcc                                    <  2:317855/149‑1 (MQ=255)                                                                                                              aCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                      >  6:100818/1‑114 (MQ=255)                                                                                                              aCCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCgg                                                                      <  5:100818/114‑1 (MQ=255)                                                                                                               cccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGcc                                    >  5:263597/1‑147 (MQ=255)                                                                                                                ccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                                <  3:196434/102‑1 (MQ=255)                                                                                                                ccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTgg                                                                                >  4:196434/1‑102 (MQ=255)                                                                                                                ccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAg                                                      <  7:296276/128‑1 (MQ=255)                                                                                                                ccTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAg                                                      >  8:296276/1‑128 (MQ=255)                                                                                                                  tCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAgg                                >  8:285617/1‑148 (MQ=255)                                                                                                                    gATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGGGGATTCTGCCTAGGCa                              >  5:281973/1‑148 (MQ=255)                                                                                                                         gTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGc                                     <  7:232298/136‑1 (MQ=255)                                                                                                                         gTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGc                                     >  8:232298/1‑136 (MQ=255)                                                                                                                             aTGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGtt                       <  3:100863/146‑1 (MQ=255)                                                                                                                             aTGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGtt                       >  4:100863/1‑146 (MQ=255)                                                                                                                               gCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCgg                  >  7:130466/1‑149 (MQ=255)                                                                                                                                cGGATCTCTCCATGGAGGCGTGGATGGTGGGTCGGACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCGCCTAGGACGGTGCGGATTCTGCCTAGGCAATAGGTTTTCGGc                 <  6:336144/149‑1 (MQ=255)                                                                                                                                       caccATGCACGCCTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCt          >  8:35430/3‑149 (MQ=255)                                                                                                                                          cATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtt       >  3:223675/1‑149 (MQ=255)                                                                                                                                          cATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGtt       >  5:151900/1‑149 (MQ=255)                                                                                                                                               aGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTATGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGTTTTTTa  >  3:148335/1‑149 (MQ=255)                                                                                                                                                |                                                                                                                                                    ACCAACAGTGATTCCAGCATCAGGATTCCTACGGGGGCGGGTCGACACGGCTATGACACGTACAGCCTCCCCGCCCCGGGTAAGGTGTGCGTGTCATAGGTCTTGTCAAACCCTCGATCCGTCTATGCGGATCTCTCCATGCAGGCGTGCATGGTGGGTCGCACGCGCCATGGTCTGTGGACCAAGTATGTTGACGCGTACCGGGCGAGCTGGGTGCTCGCGGGAGACTACTCCCCTAGGACGGTGCGGATTCTGCCTAGGCAAAACGTTTTCGGCAAGCGCTGTTTTTTA  >  NC_002947/875872‑876162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑