Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 879,675 A→G 25.0% T402A (ACC→GCC)  PP_0763 → medium‑chain‑fatty acid CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947879,6750AG25.0% 68.1 / 23.2 44T402A (ACC→GCC) PP_0763medium‑chain‑fatty acid CoA ligase
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base G (6/5);  total (22/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CGGCAGCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGATCAAGACCGGTGGCGAG  >  NC_002947/879527‑879821
                                                                                                                                                    |                                                                                                                                                  
cGGCAGCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGa                                                                                                                                                    >  7:99080/1‑149 (MQ=255)
  gCAGCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGAcc                                                                                                                                                  <  3:59104/149‑1 (MQ=255)
   cAGCGAGGATGAGCGCGTCACTTACAGTATCAAGGCCGGTGTGACGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCa                                                                                                                                                 >  6:192930/1‑149 (MQ=255)
     gCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATg                                                                                                                                               >  4:171311/1‑149 (MQ=255)
     gCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATg                                                                                                                                               <  3:350871/149‑1 (MQ=255)
               gcgcGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTc                                                                                                                                      >  7:94770/1‑148 (MQ=255)
               gcgcGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCa                                                                                                                                     <  2:166182/149‑1 (MQ=255)
                 gcgTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAg                                                                                                                                   >  7:304974/1‑149 (MQ=255)
                      aCTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGcc                                                                                                                              >  7:38002/1‑149 (MQ=255)
                       cTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGa                                                                                                                                                    >  8:176590/1‑126 (MQ=255)
                       cTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGa                                                                                                                                                    <  7:176590/126‑1 (MQ=255)
                        ttACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCg                                                                                                                             >  5:367434/1‑148 (MQ=255)
                        ttACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCgg                                                                                                                            <  8:304974/149‑1 (MQ=255)
                          aCCGTATCACGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGga                                                                                                                           <  5:55240/148‑1 (MQ=255)
                          aCCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCg                                                                                                                             >  3:241584/1‑146 (MQ=255)
                            cGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATgg                                                                                                                                              <  6:302519/127‑1 (MQ=255)
                            cGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATgg                                                                                                                                              >  5:302519/1‑127 (MQ=255)
                               aTCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAagag                                                                                                                     >  1:259742/1‑149 (MQ=255)
                                   aGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCGGAGAAGAGCgag                                                                                                                 <  2:133752/149‑1 (MQ=255)
                                   aGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCgag                                                                                                                 <  8:99080/149‑1 (MQ=255)
                                    ggCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATgg                                                                                                                                              <  6:330274/119‑1 (MQ=255)
                                    ggCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATgg                                                                                                                                              >  5:330274/1‑119 (MQ=255)
                                    ggCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCgag                                                                                                                 >  7:202732/1‑148 (MQ=255)
                                          tgtgCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCATGGAGCCGGAGAAGAGCGAGGAGCtgt                                                                                                          <  2:112258/149‑1 (MQ=255)
                                              ccggtgccgTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCa                                                                                                      <  8:38002/149‑1 (MQ=255)
                                                       ttGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCGGAGATGAGCGAGGAGCTGTGGCAGGGCggctg                                                                                             >  6:160452/1‑149 (MQ=255)
                                                       ttGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCGTGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCggctg                                                                                             >  7:93184/1‑149 (MQ=255)
                                                          gTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCggctggct                                                                                          <  1:17238/149‑1 (MQ=255)
                                                             gAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGca                                                                                       >  2:295031/1‑149 (MQ=255)
                                                             gAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGca                                                                                       <  2:259742/149‑1 (MQ=255)
                                                                      aTCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGa                                                                              <  5:192930/149‑1 (MQ=255)
                                                                          tcgACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTc                                                                          <  8:94770/149‑1 (MQ=255)
                                                                                        aaCTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACgg                                                            >  5:17392/1‑149 (MQ=255)
                                                                                        aaCTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCGTGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACgg                                                            <  8:93184/149‑1 (MQ=255)
                                                                                        aaCTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACgg                                                            >  1:3896/1‑149 (MQ=255)
                                                                                         aCTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGc                                                           <  6:17392/149‑1 (MQ=255)
                                                                                                 cccGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGGCCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCt                                                    <  6:367434/148‑1 (MQ=255)
                                                                                                 cccGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGc                                                     <  2:3896/147‑1 (MQ=255)
                                                                                                              aaaCCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCcgc                                      <  1:251701/149‑1 (MQ=255)
                                                                                                                               gTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGAtgt                     >  4:42745/1‑149 (MQ=255)
                                                                                                                               gTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGAt                       <  6:164598/147‑1 (MQ=255)
                                                                                                                                  cTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGAt                  >  5:278904/1‑149 (MQ=255)
                                                                                                                                   tgcgtgcgCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGAt                  >  5:358064/1‑148 (MQ=255)
                                                                                                                                        gcgcCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGATCAAGAc            >  2:342296/1‑149 (MQ=255)
                                                                                                                                                gCTGGCCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGATCAAGACCGGTGGCg    <  4:241584/149‑1 (MQ=255)
                                                                                                                                                gCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTCCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGAACGCATCAAGGATGTGATCAAGACCgctcgc     >  4:236399/1‑143 (MQ=255)
                                                                                                                                                  tGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGATCAAGACCGGTGGCGAg  >  5:348044/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGCAGCGAGGATGAGCGCGTCACTTACCGTATCAAGGCCGGTGTGCCGGTGCCGTTGGTCGAAGCGGCCATCGTCGACGGCGAAGGCAACTTCCTGCCCGCCGATGGTGAAACCCAGGGCGAGCTGGTACTGCGTGCGCCGTGGCTGACCATGGGCTACTTCAAGGAGCCGGAGAAGAGCGAGGAGCTGTGGCAGGGCGGCTGGCTGCACACCGGTGACGTCGCCACCCTCGACGGCATGGGCTACATCGACATCCGCGACCGCATCAAGGATGTGATCAAGACCGGTGGCGAG  >  NC_002947/879527‑879821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑