Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 906,797 T→A 6.0% L80Q (CTG→CAG)  PP_0790 → inner membrane protein AmpE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947906,7970TA 6.0% 198.1 / 2.6 67L80Q (CTG→CAG) PP_0790inner membrane protein AmpE
Reads supporting (aligned to +/- strand):  ref base T (33/30);  new base A (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

AACTGGTGCGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGATGAACCGCACAGCTTGCT  >  NC_002947/906650‑906944
                                                                                                                                                   |                                                                                                                                                   
aaCTGGTGCGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTTGCCTATGGTCTGCTGGCGTTGCCGGTGCACctgctg                                                                                                                                                    <  6:156410/149‑1 (MQ=255)
     gTGCGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCt                                                                                                                                               >  4:334807/1‑149 (MQ=255)
       gCGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGa                                                                                                                                             >  1:327694/1‑149 (MQ=255)
        cGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCAGCAGGTGcaccg                                                                                                                                            <  6:259904/149‑5 (MQ=255)
            tGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTa                                                                                                                                         >  5:34628/1‑148 (MQ=255)
              gAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTAc                                                                                                                                        <  3:334807/147‑1 (MQ=255)
              gAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAg                                                                                                                                      >  8:334939/1‑149 (MQ=255)
                  gCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTg                                                                                                                                  >  1:28435/1‑149 (MQ=255)
                    aaCGGAAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTggg                                                                                                                                >  5:255174/1‑149 (MQ=255)
                             gTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGa                                                                                                                       <  6:255174/149‑1 (MQ=255)
                              tGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGAt                                                                                                                      <  2:219730/149‑1 (MQ=255)
                                   ccATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGcc                                                                                                                   >  5:21576/1‑147 (MQ=255)
                                          ggACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCg                                                                                                          >  2:246056/1‑149 (MQ=255)
                                            aCGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCCATGCCAAGGCATCGCt                                                                                                        >  1:143031/1‑149 (MQ=255)
                                             cGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCtt                                                                                                       >  8:237619/1‑149 (MQ=255)
                                              gTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTg                                                                                                      >  2:90329/1‑149 (MQ=255)
                                              gTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTg                                                                                                      <  6:21576/149‑1 (MQ=255)
                                              gTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTg                                                                                                      <  6:34628/149‑1 (MQ=255)
                                              gTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTg                                                                                                      <  1:90329/149‑1 (MQ=255)
                                                 tgacATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTTGATGTGCTGGAGCCGGTGTCCTATGGTCTGCTGGCGTTGCCGGTGCATCTGCTGGTGCTGATCTACAGCCTGGGCCGCGTCGATGCCAAGGCATCGCTTggg                                                                                                    <  6:15283/145‑1 (MQ=255)
                                                 ggCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTggg                                                                                                    <  3:148888/148‑1 (MQ=255)
                                                 ggCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCAGCGATGCCAAGGCATCGCTTggg                                                                                                    <  1:227635/148‑1 (MQ=255)
                                                   ccATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCg                                                                                                 >  3:90230/1‑149 (MQ=255)
                                                    cATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACctgctg                                                                                                                                                    >  7:174640/1‑97 (MQ=255)
                                                    cATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGt                                                                                                >  6:796/1‑149 (MQ=255)
                                                    cATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGt                                                                                                <  5:105992/149‑1 (MQ=255)
                                                         tGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGt                                                                                           >  5:154089/1‑149 (MQ=255)
                                                          ggTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGCGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGt                                                                                           >  4:123568/1‑148 (MQ=255)
                                                           gtgtTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGa                                                                                         <  7:334939/149‑1 (MQ=255)
                                                            tgtTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGAt                                                                                        <  3:226334/149‑1 (MQ=255)
                                                             gtTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCaa                                                                                                                 <  8:141523/123‑1 (MQ=255)
                                                             gtTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCaa                                                                                                                 >  7:141523/1‑123 (MQ=255)
                                                              ttGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGc                                                                                      >  7:29391/1‑149 (MQ=255)
                                                                ggCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATc                                                                                                                                           >  2:59001/1‑94 (MQ=255)
                                                                ggCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATc                                                                                                                                           <  1:59001/94‑1 (MQ=255)
                                                                       gTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTggg                                                                                                    <  5:274979/126‑1 (MQ=255)
                                                                       gTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTggg                                                                                                    >  6:274979/1‑126 (MQ=255)
                                                                          gcgcTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCgggg                                                                          <  2:28435/149‑1 (MQ=255)
                                                                          gcgcTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCgggg                                                                          <  4:90230/149‑1 (MQ=255)
                                                                                ctgGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGAc                                                                      <  5:33063/147‑1 (MQ=255)
                                                                                  ggTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGa                                                                       >  6:72091/1‑144 (MQ=255)
                                                                                  ggTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGa                                                                       <  5:72091/144‑1 (MQ=255)
                                                                                     gtTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAgg                                                               >  7:90670/1‑149 (MQ=255)
                                                                                        gctgctgcATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGCCGTTTCGTGATGCCTggc                                                                                 >  2:32853/1‑128 (MQ=255)
                                                                                        gctgctgcATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGCCATCGCTTGGGGCGTTTCGTGATGCCTggc                                                                                 <  1:32853/128‑1 (MQ=255)
                                                                                         ctgctgcATGTGCTGGCGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAggcggc                                                           <  3:123568/149‑1 (MQ=255)
                                                                                            ctgcATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGgcggcg                                                          <  2:327694/147‑1 (MQ=255)
                                                                                             tgcATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGCTGCCAAGGCCTCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTg                                                       >  7:332373/1‑149 (MQ=255)
                                                                                               cATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGcggcgg                                                                            <  2:82403/126‑1 (MQ=255)
                                                                                               cATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGcggcgg                                                                            >  1:82403/1‑126 (MQ=255)
                                                                                               cATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCggg                                                                           <  6:7064/127‑1 (MQ=255)
                                                                                               cATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCggg                                                                           >  5:7064/1‑127 (MQ=255)
                                                                                                     cTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGc                                               <  6:154089/149‑1 (MQ=255)
                                                                                                      tGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGcc                                              >  8:272600/1‑149 (MQ=255)
                                                                                                        gAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATc                                                                                                           <  6:105760/86‑1 (MQ=255)
                                                                                                        gAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATc                                                                                                           >  5:105760/1‑86 (MQ=255)
                                                                                                                   cTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCAcgc                                                  >  7:65703/1‑132 (MQ=255)
                                                                                                                   cTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCAcgc                                                  <  8:65703/132‑1 (MQ=255)
                                                                                                                   cTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAgcgc                                        <  6:206562/142‑1 (MQ=255)
                                                                                                                   cTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAgcgc                                        >  5:206562/1‑142 (MQ=255)
                                                                                                                    tATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTggg                                >  8:163490/1‑149 (MQ=255)
                                                                                                                      tGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGAGGCCAAGGCATAGGTTGGGGGGTTTGGTGATGCCAGGCGGGGGGGGGACGCTCAGGCGGCGCGGCCACCGGCCGGGCGCGACCAGGGcc                              >  8:224926/1‑149 (MQ=255)
                                                                                                                             ctgGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCAGGTGGc                       >  4:206514/1‑149 (MQ=255)
                                                                                                                                gCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCg                     <  7:224926/148‑1 (MQ=255)
                                                                                                                                gCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCg                     <  5:796/148‑1 (MQ=255)
                                                                                                                                  gTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCCATGCCAAGGCATCGCATGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGAt                   >  6:165775/1‑148 (MQ=255)
                                                                                                                                        tcagCACCAGCAGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGATGAACCGc            >  3:272952/4‑149 (MQ=255)
                                                                                                                                           gCACCAGCAGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCAcgc                                                  <  6:355286/108‑1 (MQ=255)
                                                                                                                                           gCACCAGCAGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCAcgc                                                  >  5:355286/1‑108 (MQ=255)
                                                                                                                                                 gctgGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATTCATGGCGGCGGGGGGCCGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGATGAACCGCACAGCTTGc   >  8:238194/1‑149 (MQ=255)
                                                                                                                                                  ctgGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGATGAACCGCACAGCTTGCt  <  7:272600/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACTGGTGCGTCTGGAACGCAACGGCAAGGTGCACCCATGGTGGACGTTGGCCATCCTGGTGTTGGCGCCGGTAGCGCTGCTGGTGTTGCTGCTGCATGTGCTGGAGCCGGTGGCCTATGGTCTGCTGGCGTTGCCGGTGCACCTGCTGGTGCTGATCTACAGCCTGGGCCGCGGCGATGCCAAGGCATCGCTTGGGGCGTTTCGTGATGCCTGGCGGCGGGGGGACGATCAGGCGGCGCTGCACGCGGCCGAGCGCGACCTGGGCCTGGTGGCCGATGAACCGCACAGCTTGCT  >  NC_002947/906650‑906944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑