Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 925,901 C→T 8.1% W242* (TGG→TAG) ‡ PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947925,9010CT 8.1% 140.3 / 2.6 49W242* (TGG→TAG) ‡PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base C (20/25);  new base T (2/2);  total (22/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGC  >  NC_002947/925758‑926042
                                                                                                                                               |                                                                                                                                             
gcTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAgcgc                                                                                                                                          <  6:355019/149‑1 (MQ=255)
     gccagggccaTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTa                                                                                                                                     >  4:72786/1‑149 (MQ=255)
     gccagggccaTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCGGGGCCAGCTCGGCGGGGGAGCCCTCGGCCAGTGCCGGAGAGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCTGGCGCTCgac                                                                                                                                     <  4:9212/149‑3 (MQ=255)
             caTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACt                                                                                                                             <  3:128782/149‑1 (MQ=255)
              aTCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTg                                                                                                                            <  6:19931/149‑1 (MQ=255)
                  gCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTAc                                                                                                                        >  3:271340/1‑149 (MQ=255)
                          ggCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAg                                                                                                                >  3:312252/1‑149 (MQ=255)
                           gCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAgg                                                                                                               >  1:226206/1‑149 (MQ=255)
                               gCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGcc                                                                                                                                               <  8:232701/113‑1 (MQ=255)
                               gCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGcc                                                                                                                                               >  7:232701/1‑113 (MQ=255)
                                     atcaCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACggg                                                                                                     >  8:247614/1‑149 (MQ=255)
                                        aCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGgcgc                                                                                                  <  4:312252/149‑1 (MQ=255)
                                        aCCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGgcgc                                                                                                  >  5:298217/1‑149 (MQ=255)
                                          ccTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCtt                                                                                                <  6:34938/149‑1 (MQ=255)
                                          ccTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCtt                                                                                                <  4:285107/149‑1 (MQ=255)
                                                  gggCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCAcgcg                                                                                        <  5:23764/149‑1 (MQ=255)
                                                      gcTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGATCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGAc                                                                                    <  7:230014/149‑1 (MQ=255)
                                                          gCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCg                                                                                 <  6:298217/148‑1 (MQ=255)
                                                           cAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGc                                                                               <  2:157325/149‑1 (MQ=255)
                                                               ccTGGTCCAGCTCGGCGGGGGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGt                                                                           <  2:339626/149‑1 (MQ=255)
                                                                  ggTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTc                                                                                              >  6:316543/1‑127 (MQ=255)
                                                                  ggTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTc                                                                                              <  5:316543/127‑1 (MQ=255)
                                                                   gTCCAGCTCGGCGGGTGAGCCCTCGGCGAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCTGGCGCTc                                                                                                                                        <  3:227594/84‑1 (MQ=255)
                                                                   gTCCAGCTCGGCGGGTGAGCCCTCGGCGAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCTGGCGCTc                                                                                                                                        >  4:227594/1‑84 (MQ=255)
                                                                     ccAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACg                                                                     >  3:210094/1‑149 (MQ=255)
                                                                      cAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTg                                                                                                            <  5:191052/109‑1 (MQ=255)
                                                                      cAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTg                                                                                                            >  6:191052/1‑109 (MQ=255)
                                                                        gCTCGGCGGGTGAGCCCTCGGCCAGTGCTGGAGGGGTAACGCGACACATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCg                                                                  <  5:10120/149‑1 (MQ=255)
                                                                        gCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTg                                                                            <  5:269814/139‑1 (MQ=255)
                                                                        gCTCGGCGGGTGAGCCCTCGGCCAGAGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTg                                                                            >  6:269814/1‑139 (MQ=255)
                                                                         cTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCg                                                                  >  3:32119/1‑148 (MQ=255)
                                                                              cggGTGAGGCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACgg                                                            <  5:333016/149‑1 (MQ=255)
                                                                              cggGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACgg                                                            <  4:210094/149‑1 (MQ=255)
                                                                                     gCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTgg                                                     <  3:72786/149‑1 (MQ=255)
                                                                                       ccTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGgcg                                                   >  1:20636/1‑149 (MQ=255)
                                                                                       ccTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGgcg                                                   >  6:177922/1‑149 (MQ=255)
                                                                                              cAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGt                                            <  2:226206/149‑1 (MQ=255)
                                                                                                  gCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGt                                                                           >  6:124207/1‑114 (MQ=255)
                                                                                                  gCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGt                                                                           <  5:124207/114‑1 (MQ=255)
                                                                                                            tAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCgg                              >  8:203805/1‑149 (MQ=255)
                                                                                                            tAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCgg                              <  7:247614/149‑1 (MQ=255)
                                                                                                                    cccATCAGCGCCGCCAGGGTCGAGCGCCAGCGCGCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGcc                      <  4:271340/149‑1 (MQ=255)
                                                                                                                       aTCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGcc                      <  5:326970/146‑1 (MQ=255)
                                                                                                                       aTCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGcc                      >  6:326970/1‑146 (MQ=255)
                                                                                                                           gcgcCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATg               >  2:31214/1‑149 (MQ=255)
                                                                                                                             gccgccAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcgctgcgc                                    <  1:176275/126‑1 (MQ=255)
                                                                                                                             gccgccAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTcgctgcgc                                    >  2:176275/1‑126 (MQ=255)
                                                                                                                                gccAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGcc          <  4:32119/149‑1 (MQ=255)
                                                                                                                                   aGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAc       >  2:329562/1‑149 (MQ=255)
                                                                                                                                    agtacGAGCGCTGGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAcg      >  1:312335/5‑149 (MQ=255)
                                                                                                                                        cGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCAcgccgc  >  2:77709/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                             
GCTGGGCCAGGGCCATCAGCACGGCGGGCAGGCGATCATCACCCTGTGCAGGGCGCTTGCAGGCCTGGTCCAGCTCGGCGGGTGAGCCCTCGGCCAGTGCCGGAGGGGTAACGCGACCCATCAGCGCCGCCAGGGTCGAGCGCCAGCGCTCGTACTGCGACTGGTACTCCTCCAGGGTGGCACGGGCGCTTTCCACGCGGGACTCGGCCTGTACCACGTCGGAACGGGTGCTGGCGCCCATGTCGCTGCGCTGGCGGGCCATGCCGGCAATGTCGCCCACGCCGC  >  NC_002947/925758‑926042

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑