Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 926,423 A→G 8.9% L68P (CTG→CCG)  PP_0805 ← outer membrane efflux protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947926,4230AG 8.9% 118.5 / 2.9 47L68P (CTG→CCG) PP_0805outer membrane efflux protein
Reads supporting (aligned to +/- strand):  ref base A (29/11);  new base G (2/2);  total (32/15)
Fisher's exact test for biased strand distribution p-value = 5.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

ATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTGGAAGTTCAGG  >  NC_002947/926275‑926569
                                                                                                                                                    |                                                                                                                                                   
aTAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCa                                                                                                                                                     <  4:240193/149‑1 (MQ=255)
 tAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAg                                                                                                                                                    >  8:202107/1‑149 (MQ=255)
                   ccgGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCGGCCGTTCGGCACTGGCGAc                                                                                                                                  >  2:279984/1‑149 (MQ=255)
                   ccgGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCGGCCGTTCGGCACTGGCGAc                                                                                                                                  >  2:275335/1‑149 (MQ=255)
                   ccgGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCgcc                                                                                                                                  >  3:44298/1‑147 (MQ=255)
                    cgGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGAcc                                                                                                                                 <  5:221098/149‑1 (MQ=255)
                    cgGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCCGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGAcc                                                                                                                                 <  6:310347/149‑1 (MQ=255)
                      gAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCgg                                                                                                                               >  3:100145/1‑149 (MQ=255)
                      gAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCgg                                                                                                                               >  1:361906/1‑149 (MQ=255)
                        aaTCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCgggg                                                                                                                             <  2:361906/149‑1 (MQ=255)
                        aaTCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCgggg                                                                                                                             >  1:138379/1‑149 (MQ=255)
                           cTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCa                                                                                                                          >  3:135322/1‑149 (MQ=255)
                                    aTACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑gg                                                                                                                <  2:268864/149‑1 (MQ=255)
                                    aTACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑gg                                                                                                                <  1:189008/149‑1 (MQ=255)
                                     tACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑ggg                                                                                                               >  4:299949/1‑149 (MQ=255)
                                       ccccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCGGCCGTTCGGCACTGGCGACCGGGGCCATGCCGAT‑ggggg                                                                                                             <  1:275335/149‑1 (MQ=255)
                                       ccccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCGGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑ggggg                                                                                                             <  1:279984/149‑1 (MQ=255)
                                        cccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCAc                                                                                                                                         <  6:173274/121‑1 (MQ=255)
                                        cccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCAc                                                                                                                                         >  5:173274/1‑121 (MQ=255)
                                        cccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑gggggg                                                                                                            >  5:32322/1‑149 (MQ=255)
                                        cccGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑ggggag                                                                                                            >  7:61250/1‑147 (MQ=255)
                                           gCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGgggcg                                                                                                         <  1:253777/149‑1 (MQ=255)
                                            cACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGgggcgg                                                                                                        <  2:138379/149‑1 (MQ=255)
                                                    aCGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGAGGCGGGCGGGCAc                                                                                                <  8:61250/149‑1 (MQ=255)
                                                                         ttGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCGTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCg                                                                                         >  5:115757/1‑135 (MQ=255)
                                                                           gTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCCGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTag                                                                           >  8:226241/1‑147 (MQ=255)
                                                                            tAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGTGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGATGGGGGgggcgggcg                                                                                                     <  5:2281/121‑1 (MQ=255)
                                                                            tAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGTGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGATGGGGGgggcgggcg                                                                                                     >  6:2281/1‑121 (MQ=255)
                                                                            tAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGgggcgggcg                                                                                                     >  4:314795/1‑120 (MQ=255)
                                                                            tAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGgggcgggcg                                                                                                     <  3:314795/120‑1 (MQ=255)
                                                                             aaaGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGTT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTagcagc                                                                       >  3:31024/1‑149 (MQ=255)
                                                                               aGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGa                                                                     >  5:292384/1‑149 (MQ=255)
                                                                                    gCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGGACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGcc                                                                >  1:254726/1‑149 (MQ=255)
                                                                                       gATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGa                                                             >  7:330385/1‑149 (MQ=255)
                                                                                             cTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGgc                                                       >  3:280573/1‑149 (MQ=255)
                                                                                              tCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCgg                                                                                        >  5:106837/1‑115 (MQ=255)
                                                                                                aGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGgcg                                                      >  3:59700/1‑147 (MQ=255)
                                                                                                 gCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGCAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAg                                                   >  5:271894/1‑149 (MQ=255)
                                                                                                       ctggggTGCCTTTGGGCGGCCTCCAGCACCGGGTCGTTACGGCCCCGCCGTTCGCCACTGGCGCCGGGGGCCCTGCCTTG‑GGGGGGGGGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTgggg                                             <  4:135322/146‑1 (MQ=255)
                                                                                                           ggTGCCATTGCGCTGCCTGAAGCACCGCGTCCGTAAGGCTCAGCCGTTCGCCACTGGCGACCGCGCCCATCCCGAA‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCCCGTAGCAGCGACCGCCCGATCTGGCCCAGGTggggcac                                          >  4:176593/1‑145 (MQ=255)
                                                                                                            gTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCAc                                                                                                >  7:145317/1‑93 (MQ=255)
                                                                                                                             gccgcaCCGGGGCGGAACGCCTCCGCCGTTCGCCACTGGGTCCGGGGGCCAGGCGGTG‑GGGGGGGGGGGGGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTgg                       <  6:271894/146‑1 (MQ=255)
                                                                                                                                          ggTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTgg           >  7:343206/1‑148 (MQ=255)
                                                                                                                                          ggTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTgg           >  3:195133/1‑148 (MQ=255)
                                                                                                                                           gTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAccc                 >  3:55842/1‑141 (MQ=255)
                                                                                                                                           gTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTgg           >  3:306099/1‑147 (MQ=255)
                                                                                                                                                gCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTGGAAGtt      >  2:114329/1‑147 (MQ=255)
                                                                                                                                                  tCAGCCCTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACTGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTGGAAGTTCAgg  >  8:163654/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATAACCGGTGCGGATACCGCCGGAAATCTGTGGGTAATACCCCGCACGGGCGACGTTGATGCCTTCGCCCTGCTTGTAAAGGTTGCCGATGGCCTCAGCGATGCTCGGGTGCCATTGCGCTGCCTGCAGCACCGCGTCGGTAAGGCTCAGCCGTTCGCCACTGGCGACCGGGGCCATGCCGAT‑GGGGGGGCGGGCGGGCACCGCTTCGGGCGCATCGCGTAGCAGCGACGGCCCGATCTGGCGCAGGTGGGGGTCTATATCATCGGCTGCCTGGGCAACCGGGTGGAAGTTCAGG  >  NC_002947/926275‑926569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑