| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 955,149 | A→G | 13.2% | intergenic (+103/‑210) | cyoE → / → alaC | protoheme IX farnesyltransferase/aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 955,149 | 0 | A | G | 13.2% | 122.3 / 9.5 | 55 | intergenic (+103/‑210) | cyoE/alaC | protoheme IX farnesyltransferase/aminotransferase |
| Reads supporting (aligned to +/- strand): ref base A (20/26); new base G (2/5); total (22/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01 | |||||||||||
AGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA > NC_002947/955005‑955292 | aGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑TTCCTACAAGGt > 7:109271/1‑148 (MQ=255) gCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTc < 2:359155/149‑1 (MQ=255) gCCAGACCGCTGCGGATGTGCGGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTc < 8:247704/149‑1 (MQ=255) ccAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTcc > 7:178431/1‑149 (MQ=255) ccAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAACCG‑CTCCTTCAAGGTTcc > 4:208409/1‑149 (MQ=255) ctatttgCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCt < 1:309103/143‑1 (MQ=255) cAGACCGCTGCGGCTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGTAAACCCCGGCCACTGG‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCt < 6:361979/149‑1 (MQ=255) ccGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAg < 8:51803/149‑1 (MQ=255) cGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCt > 2:309103/1‑144 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑TTCCTACAAGGTTCCTGTAGGAGCgg < 8:109271/149‑1 (MQ=255) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAGGAACCTTGTAGGAGCgg < 5:156814/149‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAGGAACCTTGTAGGAGCgg < 1:337936/149‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAGGAACCTTGTAGGAGCg < 1:9285/148‑1 (MQ=38) cGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGc < 7:161293/147‑1 (MQ=255) gtaTGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACTAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAGGAACCTTGTAGGAGCggg < 1:233788/147‑1 (MQ=37) ggATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAGGAACCTTGTAGGAGCggg < 4:61479/149‑1 (MQ=37) gATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGt < 7:139948/149‑1 (MQ=255) cTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTAccc < 4:14946/149‑1 (MQ=255) tgatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCC‑CTCCAACAGGAACCATGTAGGATCGGGTTTACCcg > 2:41298/1‑149 (MQ=37) gatgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGACAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑GTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCcgc < 5:263995/149‑1 (MQ=255) atgaCCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCcgc < 1:178304/148‑1 (MQ=255) cttACCCACGCTGAAAGTGCTGCCTTTTTCACTAAAACCCGGGCCAGTTT‑GGGGGG‑T‑TTTTTTTTTGGGGTTA‑TTTTTCGTGTGCCGGCCTCTTGGGGGTAAAACCCG‑CTCCTACAAGGTTCCTTTTGTTGGGGTTTTACCCGCGAaga < 6:43139/147‑1 (MQ=255) gcTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑GTTTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAAAACG‑CTACTAAAATGTTAATTTAGGAGCGGGTTTACCCGCGAAGAGGCCAGc > 5:72804/1‑148 (MQ=37) tGAAACTGCTGCCTGTTTCACGAAAACCCCGCCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCAATTCGCGGGTTAACCCC‑CTCCTACAAGGTTACAGTAGGAGCGCGTTTAACCGCGAAACGGCCAGca > 1:38584/1‑146 (MQ=255) aCTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTAGGTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑AACCTACAAGGTTACTTTATGAGCTGCTTTACAAGCCAAAACGCCAGCAAAGaa > 1:103185/1‑148 (MQ=25) ctgctgCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CGGCTTCAAGGTTTCTTTTGGAGCGGGGTTTCCCGGGAAGAGGGCCGCACAGaaaaa > 3:243710/1‑149 (MQ=38) ctgcCTGTTTCACCAAAACCCCGGCCACTGG‑GCGGGGTT‑TTTTTTTTTGGGGTTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGTAGCGGGTTTACCCGCGAAGAGGCCAGCACAGaaaaa < 6:72804/147‑1 (MQ=25) cTGTTTCACGAAACCCCGGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCGGGCGTCTTGGCGTGTAAACCCG‑CTCCAACATGGTTCGTTTTGGTGCGGGTTTACCCGCGAATAGGCCAGCACAGaaaaataaaaat < 2:38584/149‑1 (MQ=255) aaaaaaaaaaCCCCGG‑CACAGTGGCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTTAACCCG‑CTGCTACAAGGTTGCGGTTGGGGCGGGGTTAACCGGGAAGAGGCCCGCCCAGAAAAATAAAAATACCt > 1:81758/7‑149 (MQ=25) tCACGAAAACCCCGGCCACTGT‑GCCGGG‑GTTTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTTAACCCC‑CGGCGACCAGGGTGGGGTTGGAGGGGGGTTTCCCGGGAAGCGGGCCGGCCAGAAAAATAAAAATTCCt > 7:62319/1‑149 (MQ=255) tCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCt < 8:178431/148‑1 (MQ=255) tCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTACCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCgg < 4:235071/106‑1 (MQ=255) tCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTACCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCgg > 3:235071/1‑106 (MQ=255) cccGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCC‑CACCGACAAGGTTGCTGTATGAGCGGGTTTACCCGCGAAGAGGCCCGCACAGAAAAATTAAAAAAACTGATTTTGaaa > 8:64559/1‑148 (MQ=37) ggCCCCTGG‑GCGGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTGCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAAt < 6:300256/149‑1 (MQ=255) gCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGTAA‑TTTTTCCTGTGCCGGCCTCTTCGCGGTAAAACCCG‑CTCCTACAAGTTTCCTGTAGTAGCGTGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATa < 7:303171/149‑1 (MQ=38) cTGT‑GCCGGG‑T‑TTTTTTTTTTGGGGTA‑TTTTTCCTTTGCCGGCCTCTTCTCGGTTAAACCCT‑CTCCTACAATGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATc < 1:34599/149‑1 (MQ=37) cctctctg‑g‑TTTTTTTTTGGGGGTA‑TTTTTCCTTTGCCGGCCTCTTCTCGGGTAAACCCG‑CTCATACAAGTATCCTGTAGTAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGAt < 3:30829/139‑1 (MQ=25) ggCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGCCCTCTTCGCGGTAAAACCCG‑CTCCAACAAGTTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCt < 3:208409/146‑1 (MQ=38) ggg‑g‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCACTTCGCGGGTAAACACGAGAACTACAAGGTTGGTGTATGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGaaaaata > 1:99251/1‑115 (MQ=25) ttttttttGGGGGTA‑TTTTTCCTGTCCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCt < 8:62319/148‑1 (MQ=255) tcttgggCCGGCCTCTTCGCTTTTAAACCCT‑CTCATAAAATTTTCTTTTATTAGCGTTTTTACCCGCTAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATc < 5:226772/142‑1 (MQ=255) gtgCCGGCCTCTTCGCTGTTAAACCCG‑CTCCTACATTTTTCCTGTAGTTGCGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATc < 1:117341/144‑1 (MQ=255) ctctTCGCGGGAAAACCCG‑CTCCTACAAGGTTCCTTTTGGAGGGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATc < 3:57329/149‑1 (MQ=39) ttCGCGGTTAAAACCG‑CTCCAAAAAGGTTCCTTTAGGAGCGGTTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAACAGTTCTAATAAAACAGGAAATTATcc < 1:189013/135‑1 (MQ=38) ttCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTTCAGGCATGCTg > 7:105174/1‑149 (MQ=255) ttCGCGGGTAAAACCC‑CGCCGACAAGGTTGCTGTTGGAGCGGGGTTTCCCGCGAAGAGGCCAGCACAGAAAAATAAAAATAACGGATTTTAAAAAAAAAGATCTGAAA‑AGTTGTAATAAAAAAGGAAATTAtgc > 2:189013/1‑132 (MQ=255) aaaCCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCAc > 1:186656/1‑149 (MQ=255) cccG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTa > 7:199091/1‑148 (MQ=255) ccG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACa > 7:96856/1‑110 (MQ=255) ccG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACa < 8:96856/110‑1 (MQ=255) g‑CTCCTACAGGAACCTTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTAtct > 2:100438/1‑148 (MQ=38) cTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGa > 3:300419/1‑149 (MQ=255) cAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCtt < 2:40785/114‑1 (MQ=255) cAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCtt > 1:40785/1‑114 (MQ=255) | AGCCAGACCGCTGCGGATGTGCTGATGACCTACGCACGCTGAAACTGCTGCCTGTTTCACGAAAACCCCGGCCACTGT‑GCCGGG‑G‑TTTTTTTTTGGGGGTA‑TTTTTCCTGTGCCGGCCTCTTCGCGGGTAAACCCG‑CTCCTACAAGGTTCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACAGAAAAATAAAAATACCTGATTTTCAAAAAATAGATCTGAAA‑AGTTCTAATAAAACAGGAAATTATCCTTTACAGGCATCCTGTTTCGGCACTATCTTCTGA > NC_002947/955005‑955292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |