Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	992,868	A→C	10.6%	intergenic (+26/‑126)	der → / → PP_0858	GTPase Der/methionine/glutamine aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	992,868	0	A	C	10.6%	137.2 / 2.3	53	intergenic (+26/‑126)	der/PP_0858	GTPase Der/methionine/glutamine aminotransferase
Reads supporting (aligned to +/- strand):  ref base A (23/24);  new base C (4/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 6.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGGCGAGAACCCGTACGAGGGTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAAGAGGGCTCCATGATCCG  >  NC_002947/992722‑993001                                                                                                                                                    |                                                                                                                                      gggCGGCGAGAACCCGTACGAGGGTAAGAAGAACACCCTTACCGATCCCCAGGTCAACAAGAAACGC‑CGCCTGATGGCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGAcc                                                                                                                                     <  3:54026/149‑1 (MQ=255)              ccGTACGAGGGTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACg                                                                                                                        >  5:238437/1‑149 (MQ=255)                    gAGGGTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCAAAGCAGCGCCATCTGGGGGCGGGCAAGACCGCCCCCACAGACCCCCttt                                                                                                                  >  7:79639/1‑149 (MQ=255)                      gggTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGa                                                                                                                <  6:257513/149‑1 (MQ=255)                       ggTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGCGCGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTg                                                                                                                 >  1:256298/1‑148 (MQ=255)                         tAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATcc                                                                                                             <  6:238437/149‑1 (MQ=255)                           agaagaACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGTCTTGCCCGCCCCca                                                                                                                              >  6:302257/1‑130 (MQ=255)                           agaagaACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGTCTTGCCCGCCCCca                                                                                                                              <  5:302257/130‑1 (MQ=255)                            gaagaaCACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCaga                                                                                                          <  5:232652/149‑1 (MQ=255)                             aagaaCACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCagag                                                                                                         >  4:178589/1‑149 (MQ=255)                                aaCACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCaa                                                                                                      >  8:27884/1‑149 (MQ=255)                                aaCACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCaa                                                                                                      <  7:27884/149‑1 (MQ=255)                                  cacCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCtt                                                                                                                   >  2:36336/1‑134 (MQ=255)                                  cacCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCtt                                                                                                                   <  1:36336/134‑1 (MQ=255)                                  cacCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCt                                                                                                                    <  5:166087/133‑1 (MQ=255)                                  cacCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCt                                                                                                                    >  6:166087/1‑133 (MQ=255)                                     ccTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATcccc                                                                                                 <  2:256298/149‑1 (MQ=255)                                     ccTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATcccc                                                                                                 >  5:185385/1‑149 (MQ=255)                                       ttACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATccccc                                                                                                >  3:97257/1‑148 (MQ=255)                                                        aacaaGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGTCTTGCCCGCCCCca                                                                                                                              <  5:289479/101‑1 (MQ=39)                                                        aacaaGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGTCTTGCCCGCCCCca                                                                                                                              >  6:289479/1‑101 (MQ=39)                                                          caaGAACCGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACACACGCTACGCAGCGCCATGTGGGGGCGGGCAAGCCCGCCCCCCCAGCCGCCCTGTGCTCCCGAGCAATCCCCCGATGGTTTCACCCGGTTTcc                                                                            >  5:289485/1‑149 (MQ=255)                                                          caaGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTcc                                                                            >  5:277252/1‑149 (MQ=255)                                                           aaGAAACGC‑CGCCTGATGTCGCACCACACGAAGGCCGAGAAGAAGCGGCGCGACAAACGCTACGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCt                                                                           <  6:289485/149‑1 (MQ=255)                                                                      gccTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCCCAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGa                                                                 >  5:268754/1‑149 (MQ=255)                                                                       ccTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGAt                                                                >  1:47383/1‑149 (MQ=255)                                                                        cTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCTGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTg                                                                          <  2:91158/138‑1 (MQ=255)                                                                           aTGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGc                                                            <  6:185385/149‑1 (MQ=255)                                                                            tGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGc                                                            >  6:245264/1‑148 (MQ=255)                                                                                 caccacAAGAAGGCCGACAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGCGGCGGGCAACACCCCCCCCACAGACGCCCTTTGATCCAGACCAATCCCCCTATTGTTTCAACCATTATCCTCACCGCTCGATCCGCTATGCt                                                      >  2:164547/1‑149 (MQ=255)                                                                                    cacaAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTc                                                                             <  1:191532/123‑1 (MQ=255)                                                                                    cacaAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTc                                                                             >  2:191532/1‑123 (MQ=255)                                                                                    cacaAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCg                                                    >  1:351199/1‑148 (MQ=255)                                                                                     acaAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGa                                                  <  6:277252/149‑1 (MQ=255)                                                                                     acaAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCATATTGTTTCAAc                                                                                    >  4:316562/1‑115 (MQ=255)                                                                                      caAGAAGGCCGAGAAGAAGCGTAGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGTCTAGAACTCCCACACAGACGCCCTTTGATCCAGAGCAATCGCGCTATTTTTTCAACGTTTTTCCTGACCGTTGGATACTCTTTGCtgtgta                                                 <  8:79639/149‑6 (MQ=255)                                                                                          aagGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCa                                             <  1:164547/149‑1 (MQ=255)                                                                                            ggCCGAGAAGTAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGCCGCCCTGTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCAcc                                           >  3:102087/1‑149 (MQ=255)                                                                                             gCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCAccc                                          >  8:217744/1‑149 (MQ=255)                                                                                              ccGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCAcccc                                         <  3:178589/149‑1 (MQ=255)                                                                                              ccGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCAcccc                                         <  8:282433/149‑1 (MQ=255)                                                                                               cGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCt                                                           >  6:347497/1‑130 (MQ=255)                                                                                               cGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCt                                                           <  5:347497/130‑1 (MQ=255)                                                                                               cGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCg                                        >  4:187724/1‑149 (MQ=255)                                                                                                        gCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCGCAGACGCCCTTTGCTGCAGAGCCATCCCCGTATTGTGTAATCGTTTTTACTGACCGCTCGATCCGATATGATCGGACTCCCCCCCGTGCGGGcta                               >  6:226422/1‑149 (MQ=255)                                                                                                          gTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGAtata                             <  2:47383/149‑1 (MQ=255)                                                                                                           tCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATAc                            <  2:351199/149‑1 (MQ=255)                                                                                                                    aCGATAAGCAGCGCCATGTGGGGGCGGGCAAGCCCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTTTTGTTTCAACCTTTTTCCTGACGCCTCGATCCGCTATGCTCGGACTCCACCACGTGCCGTATATACCCCAGGa                     >  8:245763/1‑147 (MQ=255)                                                                                                                                 ccATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAAGAGGGCTCCATGa      <  7:217744/149‑1 (MQ=255)                                                                                                                                   aTGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAAGAGGGCTCCATGATc    <  5:245264/149‑1 (MQ=255)                                                                                                                                      ggggggCGGGCAAGACCGCACCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCTCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAAGAGGGCTCCATGATCCg  <  4:102087/147‑1 (MQ=255)                                                                                                                                                 aaGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAagag                <  2:250259/123‑1 (MQ=255)                                                                                                                                                 aaGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACACCAGGAAagag                >  1:250259/1‑123 (MQ=255)                                                                                                                                                    |                                                                                                                                      GGGCGGCGAGAACCCGTACGAGGGTAAGAAGAACACCCTTACCGATCGCCAGGTCAACAAGAAACGC‑CGCCTGATGTCGCACCACAAGAAGGCCGAGAAGAAGCGTCGCGACAAACGCTAAGCAGCGCCATGTGGGGGCGGGCAAGACCGCCCCCACAGACGCCCTTTGATCCAGAGCAATCCCCCTATTGTTTCAACCTTTTTCCTGACCGCTCGATCCGCTATGCTCGGACTCCACCCCGTGCCGGATATACCCCAGGAAAGAGGGCTCCATGATCCG  >  NC_002947/992722‑993001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑