Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 996,022 T→A 7.5% Q525L (CAG→CTG)  PP_0860 ← sulfite reductase flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947996,0220TA 7.5% 184.1 / 3.4 62Q525L (CAG→CTG) PP_0860sulfite reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (29/28);  new base A (2/3);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

CTGCAGCCTGCAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAGGGCGAGCAGGG  >  NC_002947/995876‑996169
                                                                                                                                                  |                                                                                                                                                   
cTGCAGCCTGCAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTCAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCctgctg                                                                                                                                                    <  5:364623/148‑1 (MQ=255)
   cAGCCTGCAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCtgctgc                                                                                                                                                   <  7:70709/146‑1 (MQ=255)
    aGCCTGCAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGTCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCAtt                                                                                                                                               >  4:186696/1‑149 (MQ=255)
          cAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCAGCAATggcag                                                                                                                                           >  4:260238/1‑145 (MQ=255)
             gCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAggg                                                                                                                                      <  4:43088/149‑1 (MQ=255)
             gCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCAGCAATGGCAGCAgga                                                                                                                                      <  3:260238/149‑2 (MQ=255)
             gCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGATCCTGCAGCAATGGCAGCAgga                                                                                                                                      <  7:227521/149‑2 (MQ=255)
                 aGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGggcggc                                                                                                                                  <  6:266643/149‑1 (MQ=255)
                           ccGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCg                                                                                                                        <  2:55464/149‑1 (MQ=255)
                              gTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTg                                                                                                                     >  7:213201/1‑149 (MQ=255)
                                 ggCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTc                                                                                                                   <  4:83235/148‑1 (MQ=255)
                                    cTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACt                                                                                                               >  3:24938/1‑149 (MQ=255)
                                     ttGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACtt                                                                                                              >  6:292284/1‑149 (MQ=255)
                                          tGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCAcc                                                                                                         >  5:51450/1‑149 (MQ=255)
                                            ccGGGGTTGAGCAGTTCGCGGCGCACCAGTGAGCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCCCTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCgg                                                                                                       >  3:15389/1‑149 (MQ=255)
                                             cGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCggg                                                                                                      <  3:358581/149‑1 (MQ=255)
                                             cGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCggg                                                                                                      >  1:223727/1‑149 (MQ=255)
                                               gggTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCt                                                                                                    <  6:51450/149‑1 (MQ=255)
                                               gggTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCt                                                                                                    >  5:240458/1‑149 (MQ=255)
                                               gggTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACATCCACCGGGCt                                                                                                    >  4:281216/1‑149 (MQ=255)
                                                    gAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAAt                                                                                                <  3:125662/148‑1 (MQ=255)
                                                     aGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATg                                                                                               >  5:22901/1‑148 (MQ=255)
                                                     aGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATg                                                                                               >  3:266809/1‑148 (MQ=255)
                                                       cAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCAc                                                                                            <  7:20237/149‑1 (MQ=255)
                                                            cgcgGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCActgct                                                                                        <  8:213201/148‑1 (MQ=255)
                                                            cgcgGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCActgct                                                                                        <  6:22901/148‑1 (MQ=255)
                                                             gcggcgCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTgg                                                                                      <  4:155972/149‑1 (MQ=255)
                                                             gcggcgCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTgg                                                                                      >  7:52125/1‑149 (MQ=255)
                                                                   cACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACcgc                                                                                <  4:266809/149‑1 (MQ=255)
                                                                      cAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGct                                                                             >  5:240203/1‑149 (MQ=255)
                                                                        gTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGctct                                                                           >  2:260685/1‑149 (MQ=255)
                                                                             cAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCAACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAg                                                                      >  7:283063/1‑149 (MQ=255)
                                                                                tttgCAAAGGTTGGCGGATGCCAGGCGTCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCATGGCGGCCTGGTCTGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCTCGCTCTCCCAGCCa                                                                   <  4:15389/146‑1 (MQ=255)
                                                                                   gCAAAGGTTGGCGGCTGCCCGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGc                                                                >  5:102146/1‑149 (MQ=255)
                                                                                   gCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGc                                                                >  3:213215/1‑149 (MQ=255)
                                                                                   gCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGACCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGc                                                                <  6:279653/149‑1 (MQ=255)
                                                                                     aaaGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCt                                                              >  1:174948/1‑149 (MQ=255)
                                                                                     aaaGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCt                                                              >  5:116730/1‑149 (MQ=255)
                                                                                     aaaGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCt                                                              >  2:337028/1‑149 (MQ=255)
                                                                                      aaGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTg                                                             <  5:292284/149‑1 (MQ=255)
                                                                                       aGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTGGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATCCACTGCTGGCACCGCGCTCTCCCAGCCATGCCt                                                              <  4:24938/147‑1 (MQ=255)
                                                                                            ggcggcTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGc                                                        <  6:240203/148‑1 (MQ=255)
                                                                                              cggcTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGcgac                                                     <  2:223727/149‑1 (MQ=255)
                                                                                                  tGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGc                                                 <  6:240458/149‑1 (MQ=255)
                                                                                                       tgcggcCACTGGCTGGACCACGGTCAGTTTCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAAc                                            <  8:283063/148‑1 (MQ=255)
                                                                                                           gcCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCg                                          >  4:241819/1‑147 (MQ=255)
                                                                                                                  gctggACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGc                                 <  8:52125/149‑1 (MQ=255)
                                                                                                                    tggACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGCAACCGCAGAAGTGCgg                               >  8:109376/1‑149 (MQ=255)
                                                                                                                      gACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGATGAGCGAAACCGCAGAAGTGCgg                               <  6:102146/147‑1 (MQ=255)
                                                                                                                      gACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCgg                               <  3:186696/147‑1 (MQ=255)
                                                                                                                      gACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCgg                               <  1:260685/147‑1 (MQ=255)
                                                                                                                             gTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGAc                       >  2:183241/1‑148 (MQ=255)
                                                                                                                                aGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCt                                                                                                    >  3:4701/1‑68 (MQ=255)
                                                                                                                                aGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCt                                                                                                    <  4:4701/68‑1 (MQ=255)
                                                                                                                                aGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGAt                    <  2:174948/148‑1 (MQ=255)
                                                                                                                                 gTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCa                  >  2:363013/1‑149 (MQ=255)
                                                                                                                                  ttGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCAc                 >  2:152202/1‑149 (MQ=255)
                                                                                                                                      gcCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAg             >  8:240751/1‑149 (MQ=255)
                                                                                                                                       ccAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGc                                                 <  8:48268/112‑1 (MQ=255)
                                                                                                                                       ccAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGc                                                 <  8:52882/112‑1 (MQ=255)
                                                                                                                                       ccAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGc                                                 >  7:52882/1‑112 (MQ=255)
                                                                                                                                       ccAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGc                                                 >  7:48268/1‑112 (MQ=255)
                                                                                                                                             ccTGCAGCAATGGCAGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATc                   <  7:300073/136‑1 (MQ=255)
                                                                                                                                             ccTGCAGCAATGGCAGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATc                   >  8:300073/1‑136 (MQ=255)
                                                                                                                                                 ctgcCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAGGGCGAGCAggg  >  3:74932/1‑149 (MQ=255)
                                                                                                                                                 ctgcCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAGGGCGAGCAggg  <  1:183241/149‑1 (MQ=255)
                                                                                                                                                 ctgcCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAGGGCGAGCAgg   <  3:241819/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CTGCAGCCTGCAGGCCAAGCAGGTACACCGGTTGGCCTTGGCTGCCGGGGTTGAGCAGTTCGCGGCGCACCAGTGACCAGTTGGCAAAGGTTGGCGGCTGCCAGGCGGCCACTGGCTGGACACCGGTCAGTTGCGCCAGCTCCTGCTGCCATTGCTGCAGGGCGGCCTGGTCGGCGTTGTCCACTTCCACCGGGCTGAATGCACTGCTGGCACCGCGCTCTCCCAGCCATGCCTGAAGGCGACGAGCGAAACCGCAGAAGTGCGGGTACTGACGATCACCGAGGGCGAGCAGGG  >  NC_002947/995876‑996169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑