Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,001,099	A→G	6.7%	K26E (AAG→GAG)	PP_0863 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,001,099	0	A	G	6.7%	172.1 / 2.2	59	K26E (AAG→GAG)	PP_0863	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base G (2/2);  total (31/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGT  >  NC_002947/1000953‑1001242                                                                                                                                                   |                                                                                                                                                aCCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAg                                                                                                                                               <  4:12416/149‑1 (MQ=255)        ttCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGgccgcc                                                                                                                                         >  4:161232/1‑148 (MQ=255)           gCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAgg                                                                                                                                     <  8:204961/149‑1 (MQ=255)           gCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCCGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAgg                                                                                                                                     <  6:225496/149‑1 (MQ=255)               ttGCAGCTGACGGGCACCTACCATAATTTGCTGCGACGCTGGTCTGAGGGCTGATCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGcta                                                                                                                                  <  6:171786/148‑3 (MQ=255)                        aCGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTggcgg                                                                                                                         >  4:353690/1‑148 (MQ=255)                          gggCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAg                                                                                                                      >  1:298994/1‑149 (MQ=255)                                  aCCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAg                                                                                                              >  1:25298/1‑149 (MQ=255)                                  aCCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAg                                                                                                              >  3:272905/1‑149 (MQ=255)                                   ccATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCaa                                                                                                                                                >  2:44701/1‑114 (MQ=255)                                   ccATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCaa                                                                                                                                                <  1:44701/114‑1 (MQ=255)                                   ccATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAgg                                                                                                             >  6:12290/1‑149 (MQ=255)                                     aTAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGgt                                                                                                           <  3:161232/149‑1 (MQ=255)                                         tttGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTgg                                                                                                       >  6:363993/1‑149 (MQ=255)                                            gctgcGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTc                                                                                                     <  3:353690/148‑1 (MQ=255)                                              tgcGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCG‑CCAGGCGATTATTGCGGCAGCCGGGCAGGGTTTGGtagtag                                                                                                 >  1:59394/1‑144 (MQ=255)                                                cgccgcTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAg                                                                                                  <  5:363993/147‑1 (MQ=255)                                                  ccgcTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGgcg                                                                                               >  3:359903/1‑148 (MQ=255)                                                       gggCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTc                                                                                                     >  5:308017/1‑137 (MQ=255)                                                       gggCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTc                                                                                                     <  6:308017/137‑1 (MQ=255)                                                           tGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGgc                                                                                                >  1:344500/1‑138 (MQ=255)                                                           tGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGgc                                                                                                <  2:344500/138‑1 (MQ=255)                                                            gAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTgctgct                                                                                    >  2:17136/1‑149 (MQ=255)                                                             aGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTgctgct                                                                                    <  4:272905/148‑1 (MQ=255)                                                                    aGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGc                                                                               <  4:140575/146‑1 (MQ=255)                                                                      ccGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATc                                                                          >  7:197705/1‑149 (MQ=255)                                                                        gtgtCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAgg                                                                                                 >  6:112071/1‑124 (MQ=255)                                                                        gtgtCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAgg                                                                                                 <  5:112071/124‑1 (MQ=255)                                                                        gtgtCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCct                                                                        <  2:298994/149‑1 (MQ=255)                                                                          gtCCTACCTGCTGCGACGTGAGGAAGGGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCctgc                                                                      <  3:290125/149‑1 (MQ=255)                                                                           tCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTgg                                                                                                       >  6:84160/1‑115 (MQ=255)                                                                             cTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCctgctg                                                                    >  3:183789/1‑148 (MQ=255)                                                                              tACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGa                                                                  >  6:287396/1‑149 (MQ=255)                                                                                ccTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTgg                                                                                                                            >  8:180286/1‑89 (MQ=255)                                                                                ccTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTgg                                                                                                                            <  7:180286/89‑1 (MQ=255)                                                                                ccTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGa                                                                  <  4:359903/147‑1 (MQ=255)                                                                                ccTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGAt                                                                 <  1:17136/148‑1 (MQ=255)                                                                                 ctgctgCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATgg                                                               <  5:8145/149‑1 (MQ=255)                                                                                   gctgcGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGc                                                             >  5:262876/1‑149 (MQ=255)                                                                                    ctgcGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCg                                                            >  4:175876/1‑149 (MQ=255)                                                                                       cGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTg                                                          <  2:25298/148‑1 (MQ=255)                                                                                                gAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCGAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcag                                                  >  6:57064/1‑147 (MQ=255)                                                                                                gAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCGAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCcag                                                  >  6:51670/1‑147 (MQ=255)                                                                                                    tggtggATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGAt                                            <  8:197705/149‑1 (MQ=255)                                                                                                           tgtgGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCGAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACgg                                     <  5:51670/149‑1 (MQ=255)                                                                                                           tgtgGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCGAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACgg                                     <  5:57064/149‑1 (MQ=255)                                                                                                           tgtgGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACgg                                     >  4:133023/1‑149 (MQ=255)                                                                                                                ccGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGcc                                >  4:356225/1‑149 (MQ=255)                                                                                                                    cTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGc                            >  4:91649/1‑149 (MQ=255)                                                                                                                       gCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGCTGCCCGGCGtt                         >  3:248660/1‑149 (MQ=255)                                                                                                                         aGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTgg                       <  6:262876/149‑1 (MQ=255)                                                                                                                          ggCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGCGCGTGGCATCCAGCAGGATGCCACGCTTGCCCGGCggtcgt                      >  4:7566/1‑144 (MQ=255)                                                                                                                           gCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGtt                     <  4:183789/149‑1 (MQ=255)                                                                                                                             cATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCg                   <  3:175876/149‑1 (MQ=255)                                                                                                                                gCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTa                >  4:119573/1‑149 (MQ=255)                                                                                                                                  tCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACg                                      <  7:12876/125‑1 (MQ=255)                                                                                                                                  tCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACg                                      >  8:12876/1‑125 (MQ=255)                                                                                                                                       gAAACTCCCGGCAAGGCGGCCCTGGCGATTCTGGCGGCAGCGGGTCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGc         <  3:7566/149‑1 (MQ=255)                                                                                                                                            tCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGc         >  7:306960/1‑144 (MQ=255)                                                                                                                                            tCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGc         <  8:306960/144‑1 (MQ=255)                                                                                                                                              ccGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGt  >  6:294520/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                ACCATCCTTCGCTTTTGCAGCTGACGGGCACCTACCATAATTTGCTGCGCCGCTGGGCTGAGGTCTGAGCCGTGTCCTACCTGCTGCGACGTGAGGAAGTGGTGGATGTGGCCGGCTTGCAGGCCATGCTCGAGGAAACTCCCGGCAAGGCCGCCCAGGCGATTCTGGCGGCAGCGGGGCAGGGTGTGGTCGAGGCGCAGTTGCTGCTGGGGCAGATCCTGCTGGATGGGCGTGGCATCCAGCAGGATGCCACGGTTGCCCGGCGTTGGTTCGGTATCGCTGCACAGGGT  >  NC_002947/1000953‑1001242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑