| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,013,234 | C→A | 10.6% | intergenic (+62/+69) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,234 | 0 | C | A | 10.6% | 153.3 / 7.0 | 54 | intergenic (+62/+69) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (25/23); new base A (3/3); total (28/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
CGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACA > NC_002947/1013096‑1013382 | cGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAg > 4:194306/1‑149 (MQ=255) cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 3:116225/149‑1 (MQ=255) cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 2:168045/149‑1 (MQ=255) cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACATGACCTCCAGGTctg < 8:39824/149‑3 (MQ=255) gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTTGAGGTCAtg < 6:96619/149‑1 (MQ=255) gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgtg < 3:194306/149‑1 (MQ=255) tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa < 4:36760/149‑1 (MQ=255) aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa > 7:315802/1‑149 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc > 7:77226/1‑149 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc < 2:163973/149‑1 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCAGGAGGTCATGTGATaa > 4:119221/1‑148 (MQ=255) cgcTGATGGAATAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGGGGTCACGCCCGCTCCCACAGTAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 2:241154/149‑1 (MQ=255) cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 8:315802/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTTCCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGc < 5:18023/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc > 7:16392/1‑117 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGAc < 8:16392/117‑1 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt < 3:306451/134‑1 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt > 4:306451/1‑134 (MQ=255) cGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCGGTGGGAGCGGGCGTGCCCGC‑GAAgg > 8:44398/1‑149 (MQ=255) cGGACATCAAGTTCCGCGCTACCCGCGAGCAACATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAgg > 1:31866/1‑149 (MQ=255) tttttCATGTTCCGCGCTACCCGCGAGAACATTTATGGCCTTAAATCGGGGTTGCTTTGCTGCCCTTGGGGGGCACGCCCGCTCCCACAGTAATCACCAGACCTGTAGGTATTGTTATACTTGTGGTTGGGGGGGTGCCCGC‑GAAgg < 2:138572/143‑1 (MQ=255) tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATGCCCAGACCAGGAGGGCAAGTGATACCTGTGGGAGCGGGCGTGGCCGC‑GAAgggagctc > 1:138572/1‑144 (MQ=37) cAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGc > 7:18692/1‑147 (MQ=255) ccGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTGCGCGGTCACCCCCGCTCCCACACGAATCACCACACCTGCAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAggcggc < 8:72463/149‑1 (MQ=37) cccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCAttt > 3:106558/1‑148 (MQ=255) ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCCCCAGGAATCACCCCACCTGGAGGGGATGGGATACCTGTGGGCGCGGGCGTGGCCGC‑GAAGGGCCGCGAGGCGGCCCCCTTTc > 7:282557/1‑148 (MQ=37) gcgAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCCCGAGGCGGCCCCAATTCAAt > 4:126618/1‑149 (MQ=37) gAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAAttt < 7:44398/149‑1 (MQ=255) aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCgg > 1:283374/1‑149 (MQ=255) ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt > 3:53364/1‑95 (MQ=255) ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt < 4:53364/95‑1 (MQ=255) cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCg < 5:230723/101‑1 (MQ=255) cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCg > 6:230723/1‑101 (MQ=255) aGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGAt < 1:16262/149‑1 (MQ=255) aaaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg > 7:243702/1‑84 (MQ=255) aaaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 8:243702/84‑1 (MQ=255) ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCCGGAATCACCAGACCGGGAGGGCATGTGATACCTGTGGGCGCGGGCGTGCCCGC‑GACGGGCCGCTAGGCGGCCCCATTTCAAATTCCGGCTTTGCTACTGGTCCAGAt > 8:343910/1‑149 (MQ=37) cAGCCCTTCGCGGGCACGCCCCCTCCCCCCCGAAACACCAGCCCAGGCGGGGATGTGATTACGGTGGGGGCGGGCGTGCCCGC‑GACAGGCCGCGAGGCGGGCCCCATTGAATTTACGGGGTTGGTTCAGGTGCACATTagcgggcgac > 8:9130/1‑142 (MQ=255) aGCCCGTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATa < 8:282557/149‑1 (MQ=255) cccTTCGCGGGCACGCCCGCTCCCACAGTAATCACCAGACCTGGAGGTCATGTGATACCTGTGGTAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACg < 1:197966/149‑1 (MQ=255) ccTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACgg > 4:269487/1‑149 (MQ=18) gCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 3:269487/149‑1 (MQ=18) gCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc > 1:280/1‑149 (MQ=255) gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg < 8:18692/149‑1 (MQ=255) cccGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg > 2:20457/1‑149 (MQ=21) cGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 4:139327/142‑1 (MQ=255) cGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc > 3:139327/1‑142 (MQ=255) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTGGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc > 2:124661/1‑118 (MQ=17) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTGGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc < 1:124661/118‑1 (MQ=17) ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc > 7:23416/1‑149 (MQ=255) ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGGGGGAGCGGGCGTGCCCGCAGAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGGCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGc > 1:284923/1‑149 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCCC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCAAGATTAAAGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc > 4:72026/1‑149 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc > 6:260023/1‑149 (MQ=255) aaTCACCCGCCCTGGAGGTCATGTGTTACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg < 2:283374/149‑1 (MQ=255) ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa > 3:245195/1‑148 (MQ=255) ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc < 4:245195/149‑1 (MQ=255) cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc > 8:86498/1‑144 (MQ=255) cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc < 7:86498/144‑1 (MQ=255) cAGACCGGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa < 7:9130/149‑1 (MQ=255) | CGACCCGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCTAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACA > NC_002947/1013096‑1013382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |