| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,013,248 | A→T | 12.8% | intergenic (+76/+55) | prfC → / ← potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,013,248 | 0 | A | T | 12.8% | 150.3 / 11.2 | 61 | intergenic (+76/+55) | prfC/potF‑I | peptide chain release factor 3/putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (26/27); new base T (4/4); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01 | |||||||||||
CGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCA > NC_002947/1013101‑1013394 | cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 3:116225/149‑1 (MQ=255)cGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAt < 2:168045/149‑1 (MQ=255) gggTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTTGAGGTCAtg < 6:96619/149‑1 (MQ=255) gTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCAtgtg < 3:194306/149‑1 (MQ=255) tCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGa < 4:36760/149‑1 (MQ=255) aaCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATa > 7:315802/1‑149 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc > 7:77226/1‑149 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATAc < 2:163973/149‑1 (MQ=255) aCCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCAGGAGGTCATGTGATaa > 4:119221/1‑148 (MQ=255) cgcTGATGGAATAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGGGGTCACGCCCGCTCCCACAGTAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 2:241154/149‑1 (MQ=255) cgcTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 8:315802/149‑1 (MQ=255) gAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTTCCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGc < 5:18023/149‑1 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt > 4:306451/1‑134 (MQ=255) gagCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCt < 3:306451/134‑1 (MQ=255) cGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCGGTGGGAGCGGGCGTGCCCGC‑GAAgg > 8:44398/1‑149 (MQ=255) cGGACATCAAGTTCCGCGCTACCCGCGAGCAACATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAgg > 1:31866/1‑149 (MQ=255) tttttCATGTTCCGCGCTACCCGCGAGAACATTTATGGCCTTAAATCGGGGTTGCTTTGCTGCCCTTGGGGGGCACGCCCGCTCCCACAGTAATCACCAGACCTGTAGGTATTGTTATACTTGTGGTTGGGGGGGTGCCCGC‑GAAgg < 2:138572/143‑1 (MQ=255) tCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATGCCCAGACCAGGAGGGCAAGTGATACCTGTGGGAGCGGGCGTGGCCGC‑GAAgggagctc > 1:138572/1‑144 (MQ=37) cAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGc > 7:18692/1‑147 (MQ=255) ccGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTGCGCGGTCACCCCCGCTCCCACACGAATCACCACACCTGCAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAggcggc < 8:72463/149‑1 (MQ=37) cccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCAttt > 3:106558/1‑148 (MQ=255) ccGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCCCGCCCGCTCCCCCAGGAATCACCCCACCTGGAGGGGATGGGATACCTGTGGGCGCGGGCGTGGCCGC‑GAAGGGCCGC‑GAGGCGGCCCCCTTTc > 7:282557/1‑148 (MQ=37) gcgAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCCCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCCC‑GAGGCGGCCCCAATTCAAt > 4:126618/1‑149 (MQ=37) gAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCACACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAAttt < 7:44398/149‑1 (MQ=255) aCCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCgg > 1:283374/1‑149 (MQ=255) ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt > 3:53364/1‑95 (MQ=255) ccATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCtgt < 4:53364/95‑1 (MQ=255) cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCg > 6:230723/1‑101 (MQ=255) cATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCg < 5:230723/101‑1 (MQ=255) aGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGAt < 1:16262/149‑1 (MQ=255) aaaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg < 8:243702/84‑1 (MQ=255) aaaTCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTgg > 7:243702/1‑84 (MQ=255) ggggCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCCCCGGAATCACCAGACCGGGAGGGCATGTGATACCTGTGGGCGCGGGCGTGCCCGC‑GACGGGCCGC‑TAGGCGGCCCCATTTCAAATTCCGGCTTTGCTACTGGTCCAGAt > 8:343910/1‑149 (MQ=37) cAGCCCTTCGCGGGCACGCCCCCTCCCCCCCGAAACACCAGCCCAGGCGGGGATGTGATTACGGTGGGGGCGGGCGTGCCCGC‑GACAGGCCGC‑GAGGCGGGCCCCATTGAATTTACGGGGTTGGTTCAGGTGCACATTagcgggcgac > 8:9130/1‑142 (MQ=255) aGCCCGTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATa < 8:282557/149‑1 (MQ=255) cccTTCGCGGGCACGCCCGCTCCCACAGTAATCACCAGACCTGGAGGTCATGTGATACCTGTGGTAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACg < 1:197966/149‑1 (MQ=255) ccTTCGCGGGCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACgg > 4:269487/1‑149 (MQ=18) gCACGCCCGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 3:269487/149‑1 (MQ=18) gCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc > 1:280/1‑149 (MQ=255) gCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCg < 8:18692/149‑1 (MQ=255) cccGCTCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCgg > 2:20457/1‑149 (MQ=21) cGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc > 3:139327/1‑142 (MQ=255) cGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 4:139327/142‑1 (MQ=255) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TGGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc > 2:124661/1‑118 (MQ=17) tCCCACAGGTATCACATGACCTCCAGGTCTGGTGATTCCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TGGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTcgc < 1:124661/118‑1 (MQ=17) ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGcc > 7:23416/1‑149 (MQ=255) ccACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGGGGGAGCGGGCGTGCCCGCAGAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGGCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGc > 1:284923/1‑149 (MQ=255) acaGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCCC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCAAGATTAAAGTACGAATACGGTCGATCTTCGCCGGCATCGCCTc > 4:72026/1‑149 (MQ=255) gAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgc > 6:260023/1‑149 (MQ=255) aaTCACCCGCCCTGGAGGTCATGTGTTACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcg < 2:283374/149‑1 (MQ=255) ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATa > 3:245195/1‑148 (MQ=255) ccAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATAc < 4:245195/149‑1 (MQ=255) cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc < 7:86498/144‑1 (MQ=255) cAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAgcgc > 8:86498/1‑144 (MQ=255) cAGACCGGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACa < 7:9130/149‑1 (MQ=255) atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCg > 6:221567/3‑118 (MQ=25) atgACCTCCAGGTCTGGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCg < 5:221567/116‑1 (MQ=25) gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGCATAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc > 1:80190/1‑117 (MQ=255) gAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTc < 2:80190/116‑1 (MQ=255) gAGGGCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGc < 8:77226/147‑1 (MQ=255) tCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCa < 5:260023/149‑1 (MQ=255) | CGGGTCAACCTGGCGCTGATGGAAGAGCGCTGGCCGGACATCAAGTTCCGCGCTACCCGCGAGCACCATTAAGGCCTGAAATCGGGGCTGCTTTGCAGCCCTTCGCGGGCACGCCCGCTCCCACAGGAATCACCAGACCTGGAGGTCATGTGATACCTGTGGGAGCGGGCGTGCCCGC‑GAAGGGCCGC‑TAGGCGGCCCCATTTCAATTTCCGGCTTTGATACTGGTCCAGATTCGCGTACGAATACGGTCGATCTTCGCCGGCATCGCCTCCAGCGCATACAGCTTGCCCATCA > NC_002947/1013101‑1013394 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |