Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,021,167 C→T 7.4% G274S (GGT→AGT)  dppB ← dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,021,1670CT 7.4% 190.3 / 5.2 67G274S (GGT→AGT) dppBdipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (31/31);  new base T (3/2);  total (34/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

TGTCCACGACGAAGTTGACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTACTTCAAGCATC  >  NC_002947/1021020‑1021315
                                                                                                                                                   |                                                                                                                                                     
tGTCCACGACGAAGTTGACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGcaccgcaccg                                                                                                                                                      <  1:83119/149‑1 (MQ=255)
    cACGACGAAGTTGACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGc                                                                                                                                                    >  5:345899/1‑147 (MQ=255)
    cACGACGAAGTTGACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCca                                                                                                                                                  <  1:239901/149‑1 (MQ=255)
                gACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGa                                                                                                                                     >  4:273750/1‑149 (MQ=255)
                 aCCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGAc                                                                                                                                    >  3:23291/1‑149 (MQ=255)
                 aCCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGAc                                                                                                                                    <  6:345899/149‑1 (MQ=255)
                   cAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCt                                                                                                                                  >  1:128496/1‑149 (MQ=255)
                      aaTCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTg                                                                                                                                 >  2:109683/1‑147 (MQ=255)
                        tCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAgg                                                                                                                             <  4:23291/149‑1 (MQ=255)
                          aCCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGcc                                                                                                                           <  3:129040/149‑1 (MQ=255)
                          aCCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGcc                                                                                                                           >  6:1744/1‑149 (MQ=255)
                             aggcaggcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGaa                                                                                                                        >  7:296689/1‑149 (MQ=255)
                              ggcaggcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAac                                                                                                                       <  4:149142/149‑1 (MQ=255)
                                 aggcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACg                                                                                                                    <  1:109683/149‑1 (MQ=255)
                                   gcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGTACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGt                                                                                                                  >  8:317613/1‑149 (MQ=255)
                                   gcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACTGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGt                                                                                                                  >  3:104060/1‑149 (MQ=255)
                                   gcGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACTGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGt                                                                                                                  >  3:109227/1‑149 (MQ=255)
                                     gATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCa                                                                                                                <  2:128496/149‑1 (MQ=255)
                                      aTCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGTACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAg                                                                                                               <  7:317613/149‑1 (MQ=255)
                                             ggATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCggg                                                                                                        <  4:63573/149‑1 (MQ=255)
                                                   cGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAgg                                                                                                                             >  7:241977/1‑122 (MQ=255)
                                                   cGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAgg                                                                                                                             <  8:241977/122‑1 (MQ=255)
                                                   cGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAgc                                                                                                  >  1:88439/1‑149 (MQ=255)
                                                          gACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCAtt                                                                                             <  1:283716/147‑1 (MQ=255)
                                                          gACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTgc                                                                                           >  5:86781/1‑149 (MQ=255)
                                                           aCCACGGGGTAGTCACGGGCACCGATGGCTTCGATAAGCAATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTgcg                                                                                          >  4:214257/1‑149 (MQ=255)
                                                             cACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCa                                                                                        <  2:88439/149‑1 (MQ=255)
                                                                  ggTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCa                                                                                   <  6:86781/149‑1 (MQ=255)
                                                                  ggTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCa                                                                                   >  5:48903/1‑149 (MQ=255)
                                                                    tAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCTAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATg                                                                                 <  2:305897/149‑1 (MQ=255)
                                                                       tCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGAc                                                                              <  1:180890/149‑1 (MQ=255)
                                                                               aCCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAAcacca                                                                      >  6:192092/1‑149 (MQ=255)
                                                                                ccGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAAcaccac                                                                     <  6:48903/149‑1 (MQ=255)
                                                                                 cGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACcaca                                                                    >  8:11012/1‑149 (MQ=255)
                                                                                    tGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACgg                                                                 <  3:8298/149‑1 (MQ=255)
                                                                                       cTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGcc                                                              >  5:18619/1‑149 (MQ=255)
                                                                                        ttCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCg                                                             >  6:279882/1‑149 (MQ=255)
                                                                                             tCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGa                                                         >  6:395/1‑148 (MQ=255)
                                                                                                 ccATTTGCCGAGGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCTCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGc                                                    <  1:124302/149‑1 (MQ=255)
                                                                                                   aTTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCt                                                  >  5:124784/1‑149 (MQ=255)
                                                                                                     ttGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCACGCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCtt                                                 >  1:276641/1‑149 (MQ=255)
                                                                                                       gCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACTGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTg                                               <  4:104060/148‑1 (MQ=255)
                                                                                                       gCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACTGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTg                                               <  4:109227/148‑1 (MQ=255)
                                                                                                       gCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTg                                               <  5:1744/148‑1 (MQ=255)
                                                                                                       gCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTg                                               <  5:279882/148‑1 (MQ=255)
                                                                                                              cccGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCgcggg                                        <  3:273750/148‑1 (MQ=255)
                                                                                                                   ccAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGc                                                           >  7:133729/1‑124 (MQ=255)
                                                                                                                   ccAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGc                                                           <  8:133729/124‑1 (MQ=255)
                                                                                                                   ccAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGc                                  <  5:192092/149‑1 (MQ=255)
                                                                                                                     aGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCg                                 >  2:45464/1‑148 (MQ=255)
                                                                                                                      ggAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGa                               >  2:40942/1‑149 (MQ=255)
                                                                                                                      ggAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGa                               <  1:40942/149‑1 (MQ=255)
                                                                                                                      ggAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGa                               <  7:308137/149‑1 (MQ=255)
                                                                                                                      ggAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGa                               >  8:308137/1‑149 (MQ=255)
                                                                                                                       gAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGAt                              >  2:44511/1‑149 (MQ=255)
                                                                                                                        aaaaGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATg                             >  3:62596/1‑149 (MQ=255)
                                                                                                                        aaaaGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATg                             <  7:11012/149‑1 (MQ=255)
                                                                                                                        aaaaGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACATGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACAACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATg                             >  5:194152/1‑149 (MQ=255)
                                                                                                                                gTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTc                     <  5:395/149‑1 (MQ=255)
                                                                                                                                 tttCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTcg                    >  2:191497/1‑149 (MQ=255)
                                                                                                                                     ggTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCgcgc                 <  1:45464/148‑1 (MQ=255)
                                                                                                                                      gTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTcgc                   <  6:324730/145‑1 (MQ=255)
                                                                                                                                      gTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTcgc                   >  5:324730/1‑145 (MQ=255)
                                                                                                                                       tCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAg               >  1:129007/1‑148 (MQ=255)
                                                                                                                                        cAGCACCGCACTGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTa             >  5:44588/1‑149 (MQ=255)
                                                                                                                                         aGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAg                                                      >  6:19663/1‑107 (MQ=255)
                                                                                                                                         aGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAg                                                      <  5:19663/107‑1 (MQ=255)
                                                                                                                                         aGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTa             <  4:62596/148‑1 (MQ=255)
                                                                                                                                          gcaccgcaccGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCa                >  4:197211/1‑144 (MQ=255)
                                                                                                                                          gcaccgcaccGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCa                <  3:197211/144‑1 (MQ=255)
                                                                                                                                           caccgcaccgGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCACAGTACtt          >  3:80676/1‑149 (MQ=255)
                                                                                                                                             ccgcaccgGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGt                            <  3:194461/129‑1 (MQ=255)
                                                                                                                                             ccgcaccgGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGt                            >  4:194461/1‑129 (MQ=255)
                                                                                                                                                caccgGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTACTTCAAg      >  3:173489/1‑148 (MQ=255)
                                                                                                                                                   cgGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTACTTCAAGCATc  >  2:223517/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TGTCCACGACGAAGTTGACCAGAATCACCAGGCAGGCGATCAACAGGATGCCGTTCTGGACCACGGGGTAGTCACGGGCACCGATGGCTTCGATCAGCCATTTGCCGATGCCCGGCCAGGAAAAGATGGTTTCGGTCAGCACCGCACCGGCCA‑GCAGCGTGCCGACCTGCAGGCCGAACACGGTCAGCACCGGGATCAGCGCATTGCGCAGGCCATGGACGAACACCACACGGGCCGGCGACAGGCCTTTGGCGCGGGCGGTGCGGATGTAGTCTTCGCGCAGTACTTCAAGCATC  >  NC_002947/1021020‑1021315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑