Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,040,592 A→T 7.8% intergenic (+28/+14) PP_0899 → / ← PP_0900 ferredoxin reductase/PAP2 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,040,5920AT 7.8% 150.3 / 2.1 51intergenic (+28/+14)PP_0899/PP_0900ferredoxin reductase/PAP2 family protein
Reads supporting (aligned to +/- strand):  ref base A (27/20);  new base T (2/2);  total (29/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

AGACGGTGGCGCTGCTGTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCC  >  NC_002947/1040446‑1040737
                                                                                                                                                  |                                                                                                                                                 
aGACGGTGGCGCTGCTGTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAg                                                                                                                                                 >  6:314759/1‑149 (MQ=255)
 gACGGTGGCGCTGCTGTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGc                                                                                                                                                <  4:182801/149‑1 (MQ=255)
  aCGGTGGCGCTGCTGTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCg                                                                                                                                               <  1:279030/149‑1 (MQ=255)
              ctgTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCGGATGTCTTCGTCGAGCAAGCCAGAGTAGCTTTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCa                                                                                                                                   >  4:109155/1‑149 (MQ=255)
                   tgGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATgcgg                                                                                                                              >  7:245874/1‑149 (MQ=255)
                      ggCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGgcgc                                                                                                                           >  8:290722/1‑149 (MQ=255)
                              ggAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCg                                                                                                                   <  8:245874/149‑1 (MQ=255)
                               gAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCgg                                                                                                                  <  8:52639/149‑1 (MQ=255)
                                 gtgtCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTc                                                                                                                                    >  3:231949/1‑129 (MQ=255)
                                 gtgtCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTc                                                                                                                                    <  4:231949/129‑1 (MQ=255)
                                  tgtCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGa                                                                                                               >  4:63676/1‑149 (MQ=255)
                                    tCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACg                                                                                                             >  4:160717/1‑149 (MQ=255)
                                      gAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTGGAGCATGCCTGAGTAGGATTTGAAGTGCGAAGGGCCGCTTTGCGGCCCttcg                                                                                                                                       <  5:210394/121‑2 (MQ=255)
                                           gTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGACGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCt                                                                                                      >  8:230407/1‑149 (MQ=255)
                                                   gACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGc                                                                                              <  7:290722/149‑1 (MQ=255)
                                                            cgacatcAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGa                                                                                     <  2:159139/143‑1 (MQ=255)
                                                              aTGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATa                                                                                   <  5:314759/149‑1 (MQ=255)
                                                                ggCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATa                                                                                   >  5:259101/1‑147 (MQ=255)
                                                                 gCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATa                                                                                   >  2:225540/1‑146 (MQ=255)
                                                                   aGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGaa                                                                              <  3:160717/149‑1 (MQ=255)
                                                                   aGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGaa                                                                              <  3:63676/149‑1 (MQ=255)
                                                                             cgGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGccc                                                                                                                                           <  8:316699/78‑1 (MQ=255)
                                                                             cgGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGccc                                                                                                                                           >  7:316699/1‑78 (MQ=255)
                                                                              ggggACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGTCCAGCCCCCGCCGATAGAGAACCAGGTAATAc                                                                   <  1:225540/149‑1 (MQ=255)
                                                                                 gACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGTGTAGCATTTGAAGTGCGTAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGAc                                                                <  3:109155/149‑1 (MQ=255)
                                                                                  aCAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGAcc                                                               >  1:322807/1‑149 (MQ=255)
                                                                                    aGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGAccc                                                              >  4:340323/1‑148 (MQ=255)
                                                                                    aGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGAccc                                                              >  6:333498/1‑148 (MQ=255)
                                                                                         tttGCTGATGTGTACGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCAATAGAGAACCAGGTAATACGACCCCAATcc                                                        >  4:146158/1‑149 (MQ=255)
                                                                                            gCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATTCGGCGCTGCGCTCGGAGACGGGCACCTCTGCCAGCCCAAGCCGATAGAGAACCAGGTAATACGACCCCAATccta                                                      >  7:223676/1‑146 (MQ=255)
                                                                                            gCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGa                                                      >  3:113674/1‑148 (MQ=255)
                                                                                            gCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGa                                                      <  2:322807/148‑1 (MQ=255)
                                                                                              tGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTG‑AGTGCGAAGGGCCGCAAAGCGGCACCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCaa                                                  >  7:291702/1‑149 (MQ=255)
                                                                                                         tcgAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATa                                                                    >  6:207101/1‑121 (MQ=255)
                                                                                                         tcgAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATa                                                                    <  5:207101/121‑1 (MQ=255)
                                                                                                           gAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGACACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACcagc                                             >  7:300650/1‑142 (MQ=255)
                                                                                                           gAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGACACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACcagc                                             <  8:300650/142‑1 (MQ=255)
                                                                                                               aTGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGcc                                  <  6:259101/149‑1 (MQ=255)
                                                                                                                 gCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCaa                                                  <  6:264437/131‑1 (MQ=255)
                                                                                                                 gCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCaa                                                  >  5:264437/1‑131 (MQ=255)
                                                                                                                        tAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCcacaca                         >  7:54383/1‑149 (MQ=255)
                                                                                                                             tttGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCACCCAGCAGAACACTGCCGGACACACATtgtg                    >  3:88149/1‑149 (MQ=255)
                                                                                                                             tttGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATtgtg                    >  3:133820/1‑149 (MQ=255)
                                                                                                                             tttGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATtgtg                    >  4:281332/1‑149 (MQ=255)
                                                                                                                                    tGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAgg             <  3:281332/149‑1 (MQ=255)
                                                                                                                                      agaggggCCGCTTTGCGGCCCTTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCa                                                   >  3:157469/5‑109 (MQ=255)
                                                                                                                                      agaggggCCGCTTTGCGGCCCTTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCa                                                   <  4:157469/105‑1 (MQ=255)
                                                                                                                                       gAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAg          >  8:264543/1‑149 (MQ=255)
                                                                                                                                         ggggCCGCTTTGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAGTg        >  6:356843/2‑149 (MQ=255)
                                                                                                                                           ggCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAGTgcg      >  6:296302/1‑149 (MQ=255)
                                                                                                                                               gCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTAGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCTATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAGTGCGcccc  <  8:223676/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGACGGTGGCGCTGCTGTGTGGGGCACCGGGGAGTGTCGAGCGGTTTGCCCGACGCATGTTCATGGCAGGGGTACCGCGGGGACAGGTGTTTGCTGATGTGTTCGTCGAGCATGCCTGAGTAGCATTTGAAGTGCGAAGGGCCGCAAAGCGGCCCCTCTTTCATGCGGCGCTGCGCTCGGCGACGGGCACCTCGGCCAGCCCCCGCCGATAGAGAACCAGGTAATACGACCCCAATCCGATCAACCAGCAGAACACTGCCGTCCACACATTGTGCGACAGGAAGTGCGCCCC  >  NC_002947/1040446‑1040737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑