Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,094,319 G→A 7.5% P57S (CCC→TCC)  hpf ← ribosome hibernation promoting factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,094,3190GA 7.5% 151.2 / 2.2 53P57S (CCC→TCC) hpfribosome hibernation promoting factor
Reads supporting (aligned to +/- strand):  ref base G (24/25);  new base A (2/2);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGGGATCAGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACTTCTACATGCT  >  NC_002947/1094173‑1094465
                                                                                                                                                   |                                                                                                                                                  
gggggATCAGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGaa                                                                                                                                                  >  3:286479/1‑149 (MQ=255)
      tCAGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCa                                                                                                                                            <  6:10175/149‑1 (MQ=255)
      tCAGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCa                                                                                                                                            <  8:18145/149‑1 (MQ=255)
        aGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGa                                                                                                                                                   <  2:361044/140‑1 (MQ=255)
        aGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGa                                                                                                                                                   >  1:361044/1‑140 (MQ=255)
          cGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGGCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGt                                                                                                                                        <  2:99152/149‑1 (MQ=255)
                 cTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTc                                                                                                                                  >  2:224665/1‑148 (MQ=255)
                     aCCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTCTCCCGCCGGGAATCTGCAGGGTCGCCTCGAcc                                                                                                                              <  1:31541/149‑1 (MQ=255)
                       cTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTGGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTc                                                                                                                           <  8:301774/149‑1 (MQ=255)
                          gcagcaGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTctg                                                                                                                         <  4:286479/148‑1 (MQ=255)
                           cagcagGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTctgc                                                                                                                        <  8:342989/148‑1 (MQ=255)
                           cagcagGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTctgc                                                                                                                        <  1:224665/148‑1 (MQ=255)
                            agcagGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTctgct                                                                                                                       >  5:70306/1‑148 (MQ=255)
                              cagGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGaa                                                                                                                    <  1:357561/149‑1 (MQ=255)
                                   tttGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTtc                                                                                                               >  3:193074/1‑149 (MQ=255)
                                      gCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCAGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTtctc                                                                                                             >  8:65580/1‑148 (MQ=255)
                                        tGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAg                                                                                                                                           >  6:24586/1‑116 (MQ=255)
                                        tGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAg                                                                                                                                           <  5:24586/116‑1 (MQ=255)
                                               cgttGTGTTTTTTCAGTTGGCTGTCGAGCTTGTCTGCCAAGGCGTCGATCGCTTCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGTGAATCTGCAGGGTCGCCTCGACCTTCTGCTTAAGCTTCTCGACTTTAAt                                                                                                    <  5:175013/146‑1 (MQ=255)
                                               ccTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCAt                                                                                                    >  8:264358/1‑148 (MQ=255)
                                                cTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGa                                                                                                  >  3:63681/1‑149 (MQ=255)
                                                 ttGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGAAATCTGCa                                                                                                                                            >  2:85838/1‑106 (MQ=255)
                                                 ttGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGAAATCTGCa                                                                                                                                            <  1:85838/106‑1 (MQ=255)
                                                     gtTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTAcc                                                                                             >  5:277842/1‑149 (MQ=255)
                                                       tttttCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTg                                                                                           >  1:284227/1‑149 (MQ=255)
                                                              gTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTg                                                                                    <  6:70306/149‑1 (MQ=255)
                                                               ttGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTgg                                                                                   <  2:292465/149‑1 (MQ=255)
                                                                 ggCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGt                                                                                  >  6:334808/1‑148 (MQ=255)
                                                                     gTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCt                                                                                                                                               >  8:94836/1‑83 (MQ=255)
                                                                     gTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCt                                                                                                                                               <  7:94836/83‑1 (MQ=255)
                                                                        gAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGt                                                                          >  1:278830/1‑149 (MQ=255)
                                                                          gCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTc                                                                         >  7:159936/1‑148 (MQ=255)
                                                                           cTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGa                                                                       <  1:318372/149‑1 (MQ=255)
                                                                              gTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGaa                                                                      <  7:264358/147‑1 (MQ=255)
                                                                                             gATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGAAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGt                                                                          >  7:293166/1‑128 (MQ=255)
                                                                                             gATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGAAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGt                                                                          <  8:293166/128‑1 (MQ=255)
                                                                                              aTCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGc                                                    <  2:284227/149‑1 (MQ=255)
                                                                                                  cTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGc                                                >  8:316214/1‑149 (MQ=255)
                                                                                                   tGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCg                                                   <  8:159936/145‑1 (MQ=255)
                                                                                                       tACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCtttt                                            >  7:60976/1‑148 (MQ=255)
                                                                                                          aTGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAg                                        >  2:47067/1‑149 (MQ=255)
                                                                                                            gTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCttt                                             >  6:130905/1‑142 (MQ=255)
                                                                                                            gTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCttt                                             <  5:130905/142‑1 (MQ=255)
                                                                                                                     ttCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGt                                                                                  >  8:62487/1‑96 (MQ=255)
                                                                                                                     ttCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGt                                                                                  <  7:62487/96‑1 (MQ=255)
                                                                                                                              ggCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGc                     <  5:334808/148‑1 (MQ=255)
                                                                                                                                cTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTgg                  <  7:316214/149‑1 (MQ=255)
                                                                                                                                 tACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTggg                 >  1:265719/1‑149 (MQ=255)
                                                                                                                                 tACCACTTC‑GCCGCAGGGAATCTGCAGGGTCGACTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTggg                 >  3:226550/1‑149 (MQ=255)
                                                                                                                                   ccACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTggg                 <  4:193074/147‑1 (MQ=255)
                                                                                                                                      cTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACt            <  7:65580/149‑1 (MQ=255)
                                                                                                                                       ttC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACtt           >  4:179322/1‑149 (MQ=255)
                                                                                                                                                cgGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACTTCTACATGc   >  1:129630/1‑149 (MQ=255)
                                                                                                                                                 gggAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACTTCTACATGCt  >  1:290680/1‑149 (MQ=255)
                                                                                                                                                 gggAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACTTCTACATGCt  >  6:151097/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGGGGATCAGCGGGCAGCTGCACCTTGCAGCAGGCTTTGCTGTTTTTCCTTGTGTTTTTTCAGTTGGCGGTCGAGCTTGTCGGCCAAGGCGTCGATCGCTGCATACATGTCTTCGTGTTCGGCGTTGGCTACCACTTC‑GCCGCCGGGAATCTGCAGGGTCGCCTCGACCTTCTGCTGAAGCTTCTCGACTTTCATGATTACCTGCACGTTGGTGATCTTGTCGAAGTGACTCTCCACGCGGGCGAGCTTTTCAAGCACGTAATCGCGCAGTGGCTGGGTGACTTCTACATGCT  >  NC_002947/1094173‑1094465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑