Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,111,300	T→C	10.0%	intergenic (+21/‑89)	PP_0970 → / → PP_0971	cyanate MFS transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,111,300	0	T	C	10.0%	104.4 / 2.8	40	intergenic (+21/‑89)	PP_0970/PP_0971	cyanate MFS transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base C (2/2);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

CATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTG  >  NC_002947/1111154‑1111445                                                                                                                                                   |                                                                                                                                                    cATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTcc                                                                                                                                                   >  3:335897/1‑149 (MQ=255)   tgacctgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTc                                                                                                                                                 >  1:250488/1‑149 (MQ=255)     acctgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGt                                                                                                                                               >  3:295591/1‑149 (MQ=255)     acctgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGt                                                                                                                                               <  4:335897/149‑1 (MQ=255)           ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                         <  1:348499/149‑1 (MQ=255)           ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                         <  2:267419/149‑1 (MQ=255)                      aCGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCgg                                                                                                                              <  2:332699/149‑1 (MQ=255)                           aCTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTg                                                                                                                         <  3:100233/149‑1 (MQ=255)                                      tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                              >  8:271741/1‑133 (MQ=38)                                      tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                              <  7:271741/133‑1 (MQ=38)                                          tGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgg                                                                                                           <  4:295591/148‑1 (MQ=255)                                            cGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgggg                                                                                                         >  2:308561/1‑148 (MQ=255)                                               tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCaaa                                                                                                     >  7:314475/1‑147 (MQ=255)                                                               cGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTc                                                                                     <  1:146056/149‑1 (MQ=255)                                                                     gtTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAAcc                                                                                >  6:21761/1‑148 (MQ=255)                                                                                     gggcgggcGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGcccc                                                             >  3:1570/1‑149 (MQ=255)                                                                                        cgggcgCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCaa                                                          <  4:1570/149‑1 (MQ=255)                                                                                           gcgcTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGa                                                       <  4:61719/149‑1 (MQ=255)                                                                                                gCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGAt                                                  >  4:169649/1‑149 (MQ=255)                                                                                                 cATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATg                                                 >  2:59364/1‑149 (MQ=255)                                                                                                    gtgCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGcc                                               >  4:16332/1‑148 (MQ=255)                                                                                                               cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCt                                    >  5:298708/1‑148 (MQ=255)                                                                                                               cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTg                                   <  5:21761/149‑1 (MQ=255)                                                                                                                tGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGa                                  >  5:48101/1‑149 (MQ=255)                                                                                                                  cGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGAt                                 >  7:58942/1‑148 (MQ=255)                                                                                                                            tGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc                       >  7:214430/1‑148 (MQ=255)                                                                                                                              aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt                                                                                       <  4:149674/84‑1 (MQ=255)                                                                                                                              aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt                                                                                       >  3:149674/1‑84 (MQ=255)                                                                                                                              aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc                       <  3:16332/146‑1 (MQ=255)                                                                                                                                    gTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGGCTTGAGGCCCCAATGAGTGATGGCAACCGCGCCCTGATCACCCGTTTCTACCCgg                >  1:116466/1‑149 (MQ=255)                                                                                                                                        tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc           <  1:308561/148‑1 (MQ=255)                                                                                                                                        tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc           <  8:271524/148‑1 (MQ=255)                                                                                                                                        tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc           <  6:48101/148‑1 (MQ=255)                                                                                                                                            aGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcg      <  3:169649/149‑1 (MQ=255)                                                                                                                                             gTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAAATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcgc     <  8:314475/149‑1 (MQ=255)                                                                                                                                              tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc          <  3:284481/143‑1 (MQ=39)                                                                                                                                              tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc          >  4:284481/1‑143 (MQ=39)                                                                                                                                                ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGt                                                     <  5:330467/98‑1 (MQ=255)                                                                                                                                                ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCt   <  6:298708/148‑1 (MQ=255)                                                                                                                                                 cGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg  >  2:37192/1‑148 (MQ=255)                                                                                                                                                   |                                                                                                                                                    CATGACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTG  >  NC_002947/1111154‑1111445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑