| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,111,305 | G→T | 10.3% | intergenic (+26/‑84) | PP_0970 → / → PP_0971 | cyanate MFS transporter/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,111,305 | 0 | G | T | 10.3% | 110.2 / 4.6 | 39 | intergenic (+26/‑84) | PP_0970/PP_0971 | cyanate MFS transporter/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (16/19); new base T (2/2); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.30e-01 | |||||||||||
ACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCC > NC_002947/1111158‑1111451 | acctgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGt > 3:295591/1‑149 (MQ=255)acctgaccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGt < 4:335897/149‑1 (MQ=255) ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc < 1:348499/149‑1 (MQ=255) ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc < 2:267419/149‑1 (MQ=255) aCGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCgg < 2:332699/149‑1 (MQ=255) aCTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTg < 3:100233/149‑1 (MQ=255) tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg > 8:271741/1‑133 (MQ=38) tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg < 7:271741/133‑1 (MQ=38) tGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgg < 4:295591/148‑1 (MQ=255) cGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgggg > 2:308561/1‑148 (MQ=255) tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCaaa > 7:314475/1‑147 (MQ=255) cGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTc < 1:146056/149‑1 (MQ=255) gtTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAAcc > 6:21761/1‑148 (MQ=255) gggcgggcGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGcccc > 3:1570/1‑149 (MQ=255) cgggcgCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCaa < 4:1570/149‑1 (MQ=255) gcgcTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGa < 4:61719/149‑1 (MQ=255) gCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGAt > 4:169649/1‑149 (MQ=255) cATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATg > 2:59364/1‑149 (MQ=255) gtgCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGcc > 4:16332/1‑148 (MQ=255) cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCt > 5:298708/1‑148 (MQ=255) cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTg < 5:21761/149‑1 (MQ=255) tGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGa > 5:48101/1‑149 (MQ=255) cGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGAt > 7:58942/1‑148 (MQ=255) tGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc > 7:214430/1‑148 (MQ=255) aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt < 4:149674/84‑1 (MQ=255) aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt > 3:149674/1‑84 (MQ=255) aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc < 3:16332/146‑1 (MQ=255) gTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGGCTTGAGGCCCCAATGAGTGATGGCAACCGCGCCCTGATCACCCGTTTCTACCCgg > 1:116466/1‑149 (MQ=255) tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc < 1:308561/148‑1 (MQ=255) tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc < 8:271524/148‑1 (MQ=255) tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc < 6:48101/148‑1 (MQ=255) aGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcg < 3:169649/149‑1 (MQ=255) gTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAAATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcgc < 8:314475/149‑1 (MQ=255) tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc > 4:284481/1‑143 (MQ=39) tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc < 3:284481/143‑1 (MQ=39) ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGt < 5:330467/98‑1 (MQ=255) ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCt < 6:298708/148‑1 (MQ=255) cGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg > 2:37192/1‑148 (MQ=255) tCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGcc > 5:241691/1‑149 (MQ=255) | ACCTGACCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCC > NC_002947/1111158‑1111451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |