Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,111,310	T→A	11.4%	intergenic (+31/‑79)	PP_0970 → / → PP_0971	cyanate MFS transporter/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,111,310	0	T	A	11.4%	90.1 / 4.1	35	intergenic (+31/‑79)	PP_0970/PP_0971	cyanate MFS transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (15/16);  new base A (2/2);  total (17/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.24e-01

CCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGCCA  >  NC_002947/1111164‑1111458                                                                                                                                                   |                                                                                                                                                       ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                                      <  1:348499/149‑1 (MQ=255) ccGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGtgcctgc                                                                                                                                                      <  2:267419/149‑1 (MQ=255)            aCGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCgg                                                                                                                                           <  2:332699/149‑1 (MQ=255)                 aCTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTg                                                                                                                                      <  3:100233/149‑1 (MQ=255)                            tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                                           >  8:271741/1‑133 (MQ=38)                            tttttGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGCCCTCTTGGCAGGCACGAGGACgg                                                                                                                                           <  7:271741/133‑1 (MQ=38)                                tGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgg                                                                                                                        <  4:295591/148‑1 (MQ=255)                                  cGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCgggg                                                                                                                      >  2:308561/1‑148 (MQ=255)                                     tGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCaaa                                                                                                                  >  7:314475/1‑147 (MQ=255)                                                     cGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTc                                                                                                  <  1:146056/149‑1 (MQ=255)                                                           gtTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAAcc                                                                                             >  6:21761/1‑148 (MQ=255)                                                                           gggcgggcGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGcccc                                                                          >  3:1570/1‑149 (MQ=255)                                                                              cgggcgCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCaa                                                                       <  4:1570/149‑1 (MQ=255)                                                                                 gcgcTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGa                                                                    <  4:61719/149‑1 (MQ=255)                                                                                      gCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGAt                                                               >  4:169649/1‑149 (MQ=255)                                                                                       cATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATg                                                              >  2:59364/1‑149 (MQ=255)                                                                                          gtgCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGcc                                                            >  4:16332/1‑148 (MQ=255)                                                                                                     cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCt                                                 >  5:298708/1‑148 (MQ=255)                                                                                                     cTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTg                                                <  5:21761/149‑1 (MQ=255)                                                                                                      tGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGa                                               >  5:48101/1‑149 (MQ=255)                                                                                                        cGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGAt                                              >  7:58942/1‑148 (MQ=255)                                                                                                                  tGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc                                    >  7:214430/1‑148 (MQ=255)                                                                                                                    aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt                                                                                                    >  3:149674/1‑84 (MQ=255)                                                                                                                    aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGttt                                                                                                    <  4:149674/84‑1 (MQ=255)                                                                                                                    aGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTc                                    <  3:16332/146‑1 (MQ=255)                                                                                                                          gTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGGCTTGAGGCCCCAATGAGTGATGGCAACCGCGCCCTGATCACCCGTTTCTACCCgg                             >  1:116466/1‑149 (MQ=255)                                                                                                                              tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc                        <  8:271524/148‑1 (MQ=255)                                                                                                                              tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc                        <  6:48101/148‑1 (MQ=255)                                                                                                                              tAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTc                        <  1:308561/148‑1 (MQ=255)                                                                                                                                  aGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcg                   <  3:169649/149‑1 (MQ=255)                                                                                                                                   gTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAAATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAgcgc                  <  8:314475/149‑1 (MQ=255)                                                                                                                                    tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc                       >  4:284481/1‑143 (MQ=39)                                                                                                                                    tCCCGCCCTCTTGGCAGGCACGAGGACGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTcc                       <  3:284481/143‑1 (MQ=39)                                                                                                                                      ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGt                                                                  <  5:330467/98‑1 (MQ=255)                                                                                                                                      ccGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCt                <  6:298708/148‑1 (MQ=255)                                                                                                                                       cGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTg               >  2:37192/1‑148 (MQ=255)                                                                                                                                            tCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGcc         >  5:241691/1‑149 (MQ=255)                                                                                                                                                  ctgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGcc   <  5:357226/149‑1 (MQ=255)                                                                                                                                                  ctgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGc    >  2:210786/1‑148 (MQ=255)                                                                                                                                                   tgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCt                <  6:269729/135‑1 (MQ=255)                                                                                                                                                   tgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCt                >  5:269729/1‑135 (MQ=255)                                                                                                                                                   tgccAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGCCa  >  6:206641/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                                       CCGGCGGCTGGACGGCACTGGGTTGGATTTTTGCGGTGCTGGGCGTTGGCGCCGTGGTGTTCGGGCTGGGTGCCGGGCGGGCGCTGCATGTGCAGGTGACCTGCGAAAAAGTCTGAGGCCTGTTTTAGCAGTCCCGTCCTCGTGCCTGCCAAGAGGGCGGGACTGGCATAAGCCACCGGGGCAGATTATCGTGCAGTTTTCGAACCTAGGACGGAC‑‑CAGCCCCCAATGAGTGATGCCAACCGCGCCCTGATCACCCGTTTCTACCAGGCTTTCCAGCGCCTGGATGCCGACGCCA  >  NC_002947/1111164‑1111458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑