Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,113,095 G→C 9.8% A20A (GCC→GCG)  PP_0973 ← nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,113,0950GC 9.8% 118.6 / 4.7 41A20A (GCC→GCG) PP_0973nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base C (2/2);  total (21/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01

CCAGCCGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTC  >  NC_002947/1112948‑1113236
                                                                                                                                                   |                                                                                                                                             
ccagccGCTCAGCGGGTAGGCGCCCGATTCGCCGTTGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcgg                                                                                                                                              <  2:16389/149‑1 (MQ=255)
ccagccGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcgg                                                                                                                                              >  5:114195/1‑149 (MQ=255)
     cGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCgggg                                                                                                                                         >  4:7753/1‑149 (MQ=255)
           gCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCCCCGCCGtgctgcat                                                                                                                                   <  3:276344/149‑3 (MQ=255)
            cGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGGCGGCCAGGAGGTGTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCCCCGCCGtgctgcatg                                                                                                                                  <  5:89084/149‑4 (MQ=255)
              ggTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                <  3:42087/149‑1 (MQ=255)
               gTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                               <  6:25191/149‑1 (MQ=255)
                  ggCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGc                                                                                                                             <  6:114195/148‑1 (MQ=255)
                                cGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAgg                                                                                                              <  3:7753/149‑1 (MQ=255)
                                       aaaGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGa                                                                                                       <  7:273683/149‑1 (MQ=255)
                                       aaaGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGa                                                                                                       >  2:203601/1‑149 (MQ=255)
                                            cccAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcggcgg                                                                                                                                           <  5:103137/108‑1 (MQ=255)
                                            cccAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcggcgg                                                                                                                                           >  6:103137/1‑108 (MQ=255)
                                             ccAGGCCATGCCTTGCTTGGCGTTGTAGCTGTCATCGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCaa                                                                                                  <  3:120958/148‑1 (MQ=255)
                                                   cttgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGa                                                                                           >  2:237582/1‑149 (MQ=255)
                                                   cttgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGa                                                                                           >  8:43043/1‑149 (MQ=255)
                                                     tgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGAtt                                                                                         >  2:174607/1‑149 (MQ=255)
                                                       ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCttt                                                                                                                          >  2:362737/1‑114 (MQ=255)
                                                       ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCttt                                                                                                                          <  1:362737/114‑1 (MQ=255)
                                                       ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTg                                                                                        <  2:197310/148‑1 (MQ=255)
                                                        cttgcttgGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGc                                                                                      <  1:237582/149‑1 (MQ=255)
                                                                     gTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCACGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTc                                                                                                                     >  6:40240/1‑105 (MQ=255)
                                                                     gTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCACGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTc                                                                                                                     <  5:40240/105‑1 (MQ=255)
                                                                        gCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                               >  6:193283/1‑92 (MQ=255)
                                                                        gCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                               <  5:193283/92‑1 (MQ=255)
                                                                                  aGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCaaaa                                                             <  7:43043/148‑1 (MQ=255)
                                                                                     tCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTg                                                         >  4:28788/1‑149 (MQ=255)
                                                                                              aggTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCAGCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGttt                                                 >  3:35691/1‑148 (MQ=255)
                                                                                                  tttCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                <  4:340482/65‑1 (MQ=255)
                                                                                                  tttCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                >  3:340482/1‑65 (MQ=255)
                                                                                                      gATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGAc                                        <  3:28788/149‑1 (MQ=255)
                                                                                                         ggCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCt                                                                   >  6:331415/1‑119 (MQ=255)
                                                                                                         ggCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCt                                                                   <  5:331415/119‑1 (MQ=255)
                                                                                                            aTCGGAGGAAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCTGGCTTTTTGTCGATCAGGTGAAAGATGCAATGACGGATTGGCATGGAATTCTGGGCGAGCTGCAAAAGTTGCGAAGTTTAACCTAGagcgtagg                                  >  4:202571/1‑142 (MQ=255)
                                                                                                                          gTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGATGTGATGCAGTGGCCg                    >  4:291304/1‑149 (MQ=255)
                                                                                                                           ttCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCACCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGt                   <  4:35691/149‑1 (MQ=255)
                                                                                                                                aGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGtt              >  5:125806/1‑149 (MQ=255)
                                                                                                                                    gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAAcc                                             <  4:336832/114‑1 (MQ=255)
                                                                                                                                    gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAAcc                                             >  3:336832/1‑114 (MQ=255)
                                                                                                                                    gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTAtatctgct          >  5:15387/1‑142 (MQ=255)
                                                                                                                                     cgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg            >  3:254132/1‑146 (MQ=255)
                                                                                                                                     cccgatggcagccCCGCCGTGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg            >  5:122136/8‑146 (MQ=255)
                                                                                                                                      ccgatggcagccCCGCCGTGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg            >  7:212291/7‑145 (MQ=255)
                                                                                                                                       aGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGat       <  1:203601/149‑1 (MQ=255)
                                                                                                                                            gcagcaCGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTc  >  1:286913/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                             
CCAGCCGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTC  >  NC_002947/1112948‑1113236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑