Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,113,098 G→C 9.9% P19P (CCC→CCG)  PP_0973 ← nucleoid‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,113,0980GC 9.9% 120.2 / 3.2 40P19P (CCC→CCG) PP_0973nucleoid‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (18/18);  new base C (2/2);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTCGGTTGTT  >  NC_002947/1112953‑1113243
                                                                                                                                                 |                                                                                                                                                 
cGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCgggg                                                                                                                                                >  4:7753/1‑149 (MQ=255)
      gCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCCCCGCCGtgctgcat                                                                                                                                          <  3:276344/149‑3 (MQ=255)
       cGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGGCGGCCAGGAGGTGTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCCCCGCCGtgctgcatg                                                                                                                                         <  5:89084/149‑4 (MQ=255)
         ggTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                       <  3:42087/149‑1 (MQ=255)
          gTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                                      <  6:25191/149‑1 (MQ=255)
             ggCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGc                                                                                                                                    <  6:114195/148‑1 (MQ=255)
                           cGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAgg                                                                                                                     <  3:7753/149‑1 (MQ=255)
                                  aaaGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGa                                                                                                              <  7:273683/149‑1 (MQ=255)
                                  aaaGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGa                                                                                                              >  2:203601/1‑149 (MQ=255)
                                       cccAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcggcgg                                                                                                                                                  <  5:103137/108‑1 (MQ=255)
                                       cccAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCAcggcgg                                                                                                                                                  >  6:103137/1‑108 (MQ=255)
                                        ccAGGCCATGCCTTGCTTGGCGTTGTAGCTGTCATCGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCaa                                                                                                         <  3:120958/148‑1 (MQ=255)
                                              cttgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGa                                                                                                  >  2:237582/1‑149 (MQ=255)
                                              cttgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGa                                                                                                  >  8:43043/1‑149 (MQ=255)
                                                tgccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGAtt                                                                                                >  2:174607/1‑149 (MQ=255)
                                                  ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCttt                                                                                                                                 >  2:362737/1‑114 (MQ=255)
                                                  ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCttt                                                                                                                                 <  1:362737/114‑1 (MQ=255)
                                                  ccttgcTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTg                                                                                               <  2:197310/148‑1 (MQ=255)
                                                   cttgcttgGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGc                                                                                             <  1:237582/149‑1 (MQ=255)
                                                                gTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCACGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTc                                                                                                                            >  6:40240/1‑105 (MQ=255)
                                                                gTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCACGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTc                                                                                                                            <  5:40240/105‑1 (MQ=255)
                                                                   gCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                                      >  6:193283/1‑92 (MQ=255)
                                                                   gCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCgg                                                                                                                                      <  5:193283/92‑1 (MQ=255)
                                                                             aGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCaaaa                                                                    <  7:43043/148‑1 (MQ=255)
                                                                                tCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTg                                                                >  4:28788/1‑149 (MQ=255)
                                                                                         aggTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCAGCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGttt                                                        >  3:35691/1‑148 (MQ=255)
                                                                                             tttCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                       <  4:340482/65‑1 (MQ=255)
                                                                                             tttCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCg                                                                                                                                       >  3:340482/1‑65 (MQ=255)
                                                                                                 gATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGAc                                               <  3:28788/149‑1 (MQ=255)
                                                                                                    ggCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCt                                                                          >  6:331415/1‑119 (MQ=255)
                                                                                                    ggCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCt                                                                          <  5:331415/119‑1 (MQ=255)
                                                                                                       aTCGGAGGAAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCTGGCTTTTTGTCGATCAGGTGAAAGATGCAATGACGGATTGGCATGGAATTCTGGGCGAGCTGCAAAAGTTGCGAAGTTTAACCTAGagcgtagg                                         >  4:202571/1‑142 (MQ=255)
                                                                                                                     gTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGATGTGATGCAGTGGCCg                           >  4:291304/1‑149 (MQ=255)
                                                                                                                      ttCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCACCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGt                          <  4:35691/149‑1 (MQ=255)
                                                                                                                           aGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGtt                     >  5:125806/1‑149 (MQ=255)
                                                                                                                               gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAAcc                                                    >  3:336832/1‑114 (MQ=255)
                                                                                                                               gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAAcc                                                    <  4:336832/114‑1 (MQ=255)
                                                                                                                               gcgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTAtatctgct                 >  5:15387/1‑142 (MQ=255)
                                                                                                                                cgAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg                   >  3:254132/1‑146 (MQ=255)
                                                                                                                                cccgatggcagccCCGCCGTGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg                   >  5:122136/8‑146 (MQ=255)
                                                                                                                                 ccgatggcagccCCGCCGTGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTgg                   >  7:212291/7‑145 (MQ=255)
                                                                                                                                  aGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGat              <  1:203601/149‑1 (MQ=255)
                                                                                                                                       gcagcaCGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTc         >  1:286913/1‑149 (MQ=255)
                                                                                                                                              gtcggGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTCGgttgtt  >  7:234429/3‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CGCTCAGCGGGTAGGCGCCCGATTCGCCGTGGAAAAAGCCCCAGGCCTTGCCTTGCTTGGCGTTGTAGCTGTCATTGAGGTCGGCCAGGAGGTTTTCGATGGCATCGGAGGCAGCCAGTTCGGAGTCGCGAGCATGCAGCACGGCGGGGCTGCCATCGGGCTTTTTGTCGATCAGGTGAACGATGCAATGACGGATTGGCATGGAATTCTCGGCGAGCTGCAAAAGTTGCCAAGTTTAACCTAGACCTGAGGTGATGCAGTGGCCGTATGTTGGCAGATATGTCGGTTGTT  >  NC_002947/1112953‑1113243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑