Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 85,081 T→G 7.6% intergenic (+59/+71) aroE → / ← PP_0075 shikimate 5‑dehydrogenase/choline sulfate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294785,0810TG 7.6% 150.7 / 4.9 53intergenic (+59/+71)aroE/PP_0075shikimate 5‑dehydrogenase/choline sulfate transporter
Reads supporting (aligned to +/- strand):  ref base T (25/24);  new base G (2/2);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

AAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTG  >  NC_002947/84940‑85224
                                                                                                                                              |                                                                                                                                                 
aaGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGc                                                                                                                                            <  2:285237/149‑1 (MQ=255)
  gCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCg                                                                                                                                          <  6:343744/149‑1 (MQ=255)
            tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgt                                                                                                                                 <  8:84725/148‑1 (MQ=255)
            tCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgt                                                                                                                                 <  8:79743/148‑1 (MQ=255)
                gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCtgtg                                                                                                                                <  1:360221/145‑1 (MQ=255)
                gcgtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAg                                                                                                                            >  6:285450/1‑149 (MQ=255)
                  gtggcgtgAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCC‑ATCGCGACGCAAGGCCGCTCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAACgg                                                                                                                         <  1:238685/149‑1 (MQ=18)
                            gCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTc                                                                                                                >  7:272881/1‑149 (MQ=255)
                               ggATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTg                                                                                                                    <  6:142578/142‑1 (MQ=255)
                               ggATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTg                                                                                                                    >  5:142578/1‑142 (MQ=255)
                               ggATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTcgc                                                                                                              >  8:275297/1‑148 (MQ=255)
                                           ggTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCaaa                                                                                                 <  5:285450/149‑1 (MQ=255)
                                           ggTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCa                                                                                                   >  6:331222/1‑147 (MQ=255)
                                                   cTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTAGGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAc‑‑cc                                                                                       <  6:176582/149‑1 (MQ=255)
                                                       aCTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCTTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGaa                                                                                   <  7:1164/149‑1 (MQ=255)
                                                         tGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATc                                                                                 >  4:40118/1‑149 (MQ=255)
                                                          gcgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGGGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                              >  6:162708/1‑137 (MQ=255)
                                                          gcgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGAGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCa                                                                                              <  5:162708/137‑1 (MQ=255)
                                                           cgcCGGCAACTGTCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑GGTCGTACGCCGATCCTGTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATc                                                                                 <  4:222366/147‑1 (MQ=255)
                                                           cgcCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATcc                                                                                >  5:66270/1‑148 (MQ=255)
                                                                   aCTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCAt                                                                       <  5:269369/149‑1 (MQ=255)
                                                                      cgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCt                                                                          <  8:256806/143‑1 (MQ=255)
                                                                      cgctcgGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCt                                                                          >  7:256806/1‑143 (MQ=255)
                                                                           ggggCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCaaa                                                               <  6:66270/149‑1 (MQ=255)
                                                                                       gCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCggg                                                     <  3:40118/147‑1 (MQ=255)
                                                                                        ccGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGca                                                   >  7:146094/1‑148 (MQ=255)
                                                                                         tagggCCGCCAAGCGGCCCTATCGCGACGCAACGCCGTTACCACAAAGTA‑CGTCGTACGCCGCTCCACTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAACCAGAC‑‑CCAGAATCCCCACCTCAATCCTCAACCCCGATCGGGcaca                                                 >  8:139354/3‑149 (MQ=37)
                                                                                         cGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGc                                                                                                                   >  8:212209/1‑85 (MQ=255)
                                                                                           gggCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAAc                                               <  8:272881/149‑1 (MQ=255)
                                                                                              ccGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTcc                                            <  8:146094/149‑1 (MQ=255)
                                                                                                 cTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTcc                                                                    >  7:243762/1‑122 (MQ=255)
                                                                                                 cTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTcc                                                                    <  8:243762/122‑1 (MQ=255)
                                                                                                         gcccaTCGCGACGCAAGGCCGCGCCCACAGAGGATAGGCGTAAGCCG‑TACGTTGTGGGAACGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGCATCCCAACCGAATTCCTCAAACCGGATCGGGCACCACTCCACCCCGTcccg                                 >  4:88614/5‑147 (MQ=9)
                                                                                                          ccTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAAc                                                              <  7:214385/119‑1 (MQ=255)
                                                                                                          ccTATCGCGACGCAAGGCCGTTACCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAAc                                                              >  8:214385/1‑119 (MQ=255)
                                                                                                           cTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCt                               >  3:260588/1‑149 (MQ=255)
                                                                                                              tCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCt                            >  7:204603/1‑149 (MQ=255)
                                                                                                                gcgACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTg                           >  4:124887/1‑148 (MQ=255)
                                                                                                                 cgACGCAAGGCCGTACCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGTGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCACAATCCACAAACCCGATCGGGCACAACTCCACCCCTTCCAGCGTCTGc                          >  8:176599/1‑148 (MQ=39)
                                                                                                                            cgctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAc                                          <  6:143270/118‑1 (MQ=18)
                                                                                                                            cgctCCCACAGAGGATCGGCGTACGACG‑TACTTTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCAc                                          >  5:143270/4‑121 (MQ=18)
                                                                                                                             gTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                          <  3:185698/136‑1 (MQ=255)
                                                                                                                             gTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGc                          >  4:185698/1‑136 (MQ=255)
                                                                                                                              ttCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAc            >  3:293917/1‑149 (MQ=255)
                                                                                                                                cccACAAAGTA‑CGTCGTACGCCGATCCTCTGGGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCAcc           <  7:139354/148‑1 (MQ=255)
                                                                                                                                 ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa                <  3:106905/142‑1 (MQ=255)
                                                                                                                                 ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGa                >  4:106905/1‑142 (MQ=255)
                                                                                                                                 ccACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTg         >  5:326430/1‑149 (MQ=255)
                                                                                                                                  cacaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGAGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGc        >  1:44496/1‑149 (MQ=255)
                                                                                                                                   acaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCg       <  8:204603/149‑1 (MQ=255)
                                                                                                                                   acaAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCg       >  6:29826/1‑149 (MQ=255)
                                                                                                                                        gTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGACTACCAGAATCCCAACCTCATTCCTCAAACCGGAACGGGCACACCTCCACCCATGCCACGTGGTGCACCACCACGATCACATGCGGca    >  1:103305/1‑148 (MQ=25)
                                                                                                                                        gTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTg  <  4:260588/149‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
AAGCCTTCTTTATCTGGCGTGGCGTGAGGCCGGATACGGCGCCGGTGTTGGCTGAACTGCGCCGGCAACTCGCTCGGGGCTGAGCTGGCCGGGGCCGCTTGGCGGCCCTATCGCGACGCAAGGCCGTTCCCACAAAGTA‑CGTCGTACGCCGATCCTCTGTGGGAGCGGCCTTGCGTCGCGATGGGCTGCAAAGCAGAC‑‑CCAGAATCCCAACCTCATTCCTCAAACCGGATCGGGCACAACTCCACCCCTTCCAGCTTCTGCAACTCCTCGATCACCTGCGGCCTG  >  NC_002947/84940‑85224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑