Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,135,615	C→A	9.4%	E454* (GAA→TAA)	PP_0997 ←	sigma‑54 dependent transcriptional regulator/sensory box protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,135,615	0	C	A	9.4%	181.0 / 6.6	61	E454* (GAA→TAA)	PP_0997	sigma‑54 dependent transcriptional regulator/sensory box protein
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base A (2/4);  total (27/34)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135468‑1135760                                                                                                                                                    |                                                                                                                                                   aTGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTc                                                                                                                                                    <  2:120891/148‑1 (MQ=255) aTGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTc                                                                                                                                                    >  1:19948/1‑148 (MQ=255)    gTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAgg                                                                                                                                                <  1:212156/149‑1 (MQ=255)    gTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAg                                                                                                                                                 <  7:306671/148‑1 (MQ=255)         ccGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgta                                                                                                                                           <  6:113453/149‑3 (MQ=255)          cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGc                                                                                                                                               >  6:327896/1‑144 (MQ=255)          cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGc                                                                                                                                               <  5:327896/144‑1 (MQ=255)          cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          <  6:347308/149‑4 (MQ=255)          cGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGAGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgtag                                                                                                                                          <  2:281757/149‑4 (MQ=255)                ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTc                                                                                                                                     <  1:220143/148‑1 (MQ=255)                ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCg                                                                                                                                    <  7:210304/149‑1 (MQ=255)                ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCg                                                                                                                                    <  3:49583/149‑1 (MQ=255)                ttCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCg                                                                                                                                    <  3:44803/149‑1 (MQ=255)                 tCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTTGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            >  1:113672/1‑139 (MQ=255)                 tCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGGAACAGGCTgt                                                                                                                                            <  2:113672/140‑2 (MQ=255)                  ccATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGcc                                                                                                                                  >  1:75343/1‑149 (MQ=255)                     tatTTTCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCTCCCGGCCAGTTTCCGTGTTGACTGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTTTTCCAGGCTGGCCACTTCGCCGTc                                                                                                                               <  8:12968/149‑1 (MQ=255)                      atTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCt                                                                                                                              >  2:342125/1‑149 (MQ=255)                       tttACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                             <  1:115263/149‑1 (MQ=255)                       tttACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCtt                                                                                                                             <  2:19948/149‑1 (MQ=255)                          aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           >  1:33123/1‑132 (MQ=255)                          aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           >  1:27821/1‑132 (MQ=255)                          aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           <  2:27821/132‑1 (MQ=255)                          aCGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGc                                                                                                                                           <  2:33123/132‑1 (MQ=255)                            gCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcg                                                                                                                        >  3:295190/1‑149 (MQ=255)                              aGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCcgcg                                                                                                                      >  1:190118/1‑149 (MQ=255)                                             gCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGcc                                                                                                       <  4:139420/149‑1 (MQ=255)                                                 tgtgCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCg                                                                                                   <  1:58289/149‑1 (MQ=255)                                                            tGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGt                                                                                         <  4:295190/148‑1 (MQ=255)                                                             gCAAGCGCGCGGCCAGTTGGCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTc                                                                                        <  8:149308/148‑1 (MQ=255)                                                                   gcCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGata                                                                                 <  2:190118/149‑1 (MQ=255)                                                                   gcCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTACAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCAGGCGATATCCAGGTCGCCGata                                                                                 >  5:298820/1‑149 (MQ=255)                                                                         ccAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCa                                                                                            >  8:237737/1‑132 (MQ=255)                                                                         ccAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCa                                                                                            <  7:237737/132‑1 (MQ=255)                                                                                    gtTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGata                                                                                                >  2:343514/1‑117 (MQ=255)                                                                                    gtTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGata                                                                                                <  1:343514/117‑1 (MQ=255)                                                                                       gACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGc                                                              <  6:197647/148‑1 (MQ=255)                                                                                          gggTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGAt                                                          <  1:342125/149‑1 (MQ=255)                                                                                                   gCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggc                                                 >  5:304466/1‑149 (MQ=255)                                                                                                     gTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcg‑c                                              >  2:169434/1‑149 (MQ=255)                                                                                                        taAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGa                                           <  1:169434/149‑1 (MQ=255)                                                                                                         aaGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcg‑cgg                                            >  1:327855/1‑147 (MQ=255)                                                                                                          aGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGc                                                       >  8:61105/1‑136 (MQ=255)                                                                                                          aGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGc                                                       <  7:61105/136‑1 (MQ=255)                                                                                                            cgcTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCcgcggcg‑cgg                                            >  5:353179/1‑144 (MQ=255)                                                                                                              cTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCa                                     >  1:12979/1‑149 (MQ=255)                                                                                                                   aCAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTc                                >  3:235744/1‑149 (MQ=255)                                                                                                                      gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCAc                                    <  7:168599/142‑1 (MQ=255)                                                                                                                      gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCAc                                    >  8:168599/1‑142 (MQ=255)                                                                                                                      gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCt                               >  6:42538/1‑147 (MQ=255)                                                                                                                      gTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCt                               <  5:42538/147‑1 (MQ=255)                                                                                                                          gcgcTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACt                         >  8:340472/1‑149 (MQ=255)                                                                                                                                gAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGgcgc                    <  2:12979/148‑1 (MQ=255)                                                                                                                                         tACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCg          >  7:166684/1‑149 (MQ=255)                                                                                                                                           cAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCgg         <  6:353179/148‑1 (MQ=255)                                                                                                                                             gCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGc                             >  5:134037/1‑126 (MQ=255)                                                                                                                                             gCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGc                             <  6:134037/126‑1 (MQ=255)                                                                                                                                             gCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc       >  5:286424/1‑148 (MQ=255)                                                                                                                                              ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGcc       >  6:339120/1‑147 (MQ=255)                                                                                                                                              ccTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCaa     <  6:304466/149‑1 (MQ=255)                                                                                                                                               cTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAg    >  2:256159/1‑149 (MQ=255)                                                                                                                                                 gTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAggg  >  6:12512/1‑149 (MQ=255)                                                                                                                                                  ttCCATGCTTGGCACTTAGGCGTCTTTCCCGCGTGCCAACGAGGTTGCGGCGTTATGCATGTCGCCGTTATCCAACATGTTGCCCTAGCAGAAAGCCGCGGCGTCGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAgg   <  1:256159/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   ATGGTTTCCCGGGTATTCCATATTTACGCAGCCTCTGCGCGATAGCCGTGTGCGAAGTTTGCAAGCGCCCGGCCAGTTGCCGTGTTGACGGGTAGCTGGCGTATAAGCGCTGTAACAGTTCGCGCTCGAAATCACCTACAGCCTGTTCCAGGCTGGCCACTTCGCCGTCTTGCCCGCGTGCCACCGAGGTGCCGGCGATATCCAGGTCGCCGATATCCACCAGGTTGCCCTCGCAGATAGCCGCGGCG‑CGGAAGATCACGTTCTGCAACTGGCGCACATTGCCCGGCCAAGGG  >  NC_002947/1135468‑1135760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑