Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,231,588 T→A 9.4% E375D (GAA→GAT)  glpD ← aerobic glycerol‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,231,5880TA 9.4% 175.7 / 6.2 63E375D (GAA→GAT) glpDaerobic glycerol‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (24/33);  new base A (3/3);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

GTCGACAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCGGC  >  NC_002947/1231441‑1231726
                                                                                                                                                    |                                                                                                                                          
gTCGACAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTCCCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGAtt                                                                                                                                           >  1:275167/1‑149 (MQ=255)
  cGACAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCg                                                                                                                                         >  5:49590/1‑149 (MQ=255)
   gACAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTAGCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCgg                                                                                                                                        <  7:317407/149‑1 (MQ=255)
    aCAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGc                                                                                                                                       >  6:80717/1‑149 (MQ=255)
    aCAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGc                                                                                                                                       <  6:49590/149‑1 (MQ=255)
      aGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGc                                                                                                                                       >  8:126824/1‑147 (MQ=255)
        gCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGc                                                                                                                                   >  1:330569/1‑149 (MQ=255)
         cAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGAATCGGCCatgg                                                                                                                                  <  3:214265/149‑4 (MQ=255)
           aCCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGAATCGGCCatggct                                                                                                                                <  1:66386/149‑6 (MQ=255)
            ccAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGc                                                                                                                               >  6:214279/1‑149 (MQ=255)
            ccAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGc                                                                                                                               >  3:58074/1‑149 (MQ=255)
            ccAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGc                                                                                                                               <  1:274045/149‑1 (MQ=255)
               gCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGt                                                                                                                            <  7:126824/149‑1 (MQ=255)
                ccGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTgggg                                                                                                                             >  6:91928/1‑147 (MQ=255)
                       gTGCCAGTACGGCATCGATCAGCGCCTTTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGgtggtg                                                                                                                    <  3:251412/149‑1 (MQ=255)
                       gTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGgtggtg                                                                                                                    <  4:116484/149‑1 (MQ=255)
                       gTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGgtggtg                                                                                                                    <  1:245063/149‑1 (MQ=255)
                          ccAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAg                                                                                                                  <  4:58074/148‑1 (MQ=255)
                                 ggCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGGGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTgccgc                                                                                                          <  1:113413/149‑1 (MQ=255)
                                    aTCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCAGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGccgccg                                                                                                        >  6:129077/1‑148 (MQ=255)
                                     tCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGAATCGGCCatgg                                                                                                                                  <  6:231300/121‑4 (MQ=255)
                                     tCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGAATCGGCCatgg                                                                                                                                  >  5:231300/1‑118 (MQ=255)
                                         tCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACAcc                                                                                                  <  7:223319/149‑1 (MQ=255)
                                          cAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACAcc                                                                                                  >  2:322112/1‑148 (MQ=255)
                                           aGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGa                                                                                                >  1:137211/1‑149 (MQ=255)
                                            gcgcCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGAc                                                                                               <  1:322112/149‑1 (MQ=255)
                                               ccTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGa                                                                                                                   <  1:168417/126‑1 (MQ=255)
                                               ccTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGa                                                                                                                   >  2:168417/1‑126 (MQ=255)
                                                   tACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGAcagcag                                                                                          <  4:214840/147‑1 (MQ=255)
                                                      cGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTg                                                                                     >  5:99746/1‑149 (MQ=255)
                                                       gTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGc                                                                                    <  2:330569/149‑1 (MQ=255)
                                                        ttGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGcc                                                                                   >  7:184864/1‑149 (MQ=255)
                                                           tCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTgg                                                                                >  3:266476/1‑149 (MQ=255)
                                                           tCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTg                                                                                 <  2:275167/148‑1 (MQ=255)
                                                                  ctctGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACAcc                                                                                                  <  6:8610/124‑1 (MQ=255)
                                                                  ctctGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACAcc                                                                                                  >  5:8610/1‑124 (MQ=255)
                                                                       cgcCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGcc                                                                    <  1:170321/149‑1 (MQ=255)
                                                                        gcCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGcc                                                                    >  2:133839/1‑148 (MQ=255)
                                                                           cGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGaaa                                                                <  5:323531/149‑1 (MQ=255)
                                                                                gTGGCGCACTGGCGGTCCTGGGGGCGCGCATCTTTGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTTCCGGGCCAACCTCTGCCGAAAGGGc                                                            <  7:55556/148‑1 (MQ=255)
                                                                                gTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGc                                                            <  4:266476/148‑1 (MQ=255)
                                                                                   gcgcACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAgt                                                        <  2:137211/149‑1 (MQ=255)
                                                                                     gcACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAgtgt                                                      <  6:99746/149‑1 (MQ=255)
                                                                                      cACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAgtgtg                                                     <  8:184864/149‑1 (MQ=255)
                                                                                        cTGGCTGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTa                                                   <  5:95648/149‑1 (MQ=255)
                                                                                              gTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCg                                             >  5:85588/1‑149 (MQ=255)
                                                                                               tCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCgg                                            <  5:80717/149‑1 (MQ=255)
                                                                                                       gcgcgcATCTGAGTGAAGAAGGGCTTGAGTTACAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCCGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCAccg                                     >  1:36862/1‑149 (MQ=255)
                                                                                                            cATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAg                               <  6:85588/149‑1 (MQ=255)
                                                                                                                  aGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGt                         <  4:80872/149‑1 (MQ=255)
                                                                                                                     gaagaaGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTcc                       >  6:323731/1‑148 (MQ=255)
                                                                                                                       agaagGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTTGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGa                     <  2:36862/148‑1 (MQ=255)
                                                                                                                                 gAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTg           <  5:214279/148‑1 (MQ=255)
                                                                                                                                  aGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGca         <  5:129077/149‑1 (MQ=255)
                                                                                                                                   gTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAggg                             >  5:206542/1‑128 (MQ=255)
                                                                                                                                   gTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAggg                             <  6:206542/128‑1 (MQ=255)
                                                                                                                                    tt‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGca         >  1:288109/1‑147 (MQ=255)
                                                                                                                                        caagctGGCCGAATCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCg    >  6:300896/6‑149 (MQ=255)
                                                                                                                                        caagctGGCCGAATCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCg    >  1:292087/6‑149 (MQ=255)
                                                                                                                                          ccATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCGGc  >  3:154052/1‑149 (MQ=255)
                                                                                                                                            aTGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGc                                                                     <  1:161825/80‑1 (MQ=255)
                                                                                                                                            aTGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGc                                                                     >  2:161825/1‑80 (MQ=255)
                                                                                                                                               gCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACtcgtc               <  3:85130/131‑1 (MQ=255)
                                                                                                                                               gCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACtcgtc               >  4:85130/1‑131 (MQ=255)
                                                                                                                                                    |                                                                                                                                          
GTCGACAGGCAACCAGCCGCAACGTGCCAGTACGGCATCGATCAGCGCCTGTACCGTTGTCATGCCCTCTGCGCCCGGTAGTGGCGCACTGGCGGTCCAGGGGGCGCGCATCTGAGTGAAGAAGGGCTTGAGTT‑CAGCCATGGCCGATTCGGCCAGCTTGCGGTAGGTGGTGAGCTTGCCGCCGAACACCGACAGCAGCGGTGCCTGGCCAACCTCTGCCGAAAGGGCGAGTGTGTAGTCCCGGGTCACCGCCGAAGGGTTGTCCGACTCGTCGTTGCACAGCGGC  >  NC_002947/1231441‑1231726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑