Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,294,413 C→T 9.8% R11Q (CGG→CAG)  PP_1130 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,294,4130CT 9.8% 191.0 / 9.0 71R11Q (CGG→CAG) PP_1130hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (29/35);  new base T (4/3);  total (33/38)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.26e-02

CAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTACCACCAT  >  NC_002947/1294271‑1294560
                                                                                                                                              |                                                                                                                                                   
cAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAgg                                                                                                                                               >  1:198980/1‑149 (MQ=255)
 aaCGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGc                                                                                                                                              <  5:18562/149‑1 (MQ=255)
      cAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgt                                                                                                                                         <  2:31249/149‑3 (MQ=255)
        ggTCGCGGTCCATCATGCGGCGCATGCGCACATACATCCACCAGTGGAAATACAGTCCCGCAAGTGTGGTGACCTCGACGATTAAGTAATCCATGGCTATTCTTGGTCTCTCTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTAcgc                                                                                                                                       <  2:100161/149‑3 (MQ=255)
           cgcgGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCgg                                                                                                                                    <  6:266186/149‑1 (MQ=255)
            gcgGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGc                                                                                                                                   <  5:181181/149‑1 (MQ=255)
                  catccaGCGGCGCATGCGCACATACAACCACCAGTCGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCtgaat                                                                                                                              >  8:198165/1‑148 (MQ=255)
                    tccaGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgtcgaa                                                                                                                                     <  5:368483/139‑7 (MQ=255)
                    tccaGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCTGCCGGGCTGtgtcgaa                                                                                                                                     >  6:368483/1‑133 (MQ=255)
                    tccaGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCtgaatgaa                                                                                                                           <  7:327285/149‑1 (MQ=255)
                     ccaGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATTGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGaaa                                                                                                                          <  7:288110/149‑1 (MQ=255)
                      caGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCTCAACGGTGGTGACCACGACGATGAAGTAATCCATGTCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGaaa                                                                                                                          <  6:337964/148‑1 (MQ=255)
                      caGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGaaa                                                                                                                          <  2:198980/148‑1 (MQ=255)
                       aGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGaaaa                                                                                                                         >  6:297313/1‑148 (MQ=255)
                                  cgcACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTAc                                                                                                             <  6:262764/149‑1 (MQ=255)
                                    cacaTACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGt                                                                                                           <  4:127930/149‑1 (MQ=255)
                                      caTACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGAAATCCATGTCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCGCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGttt                                                                                                         <  8:279150/149‑1 (MQ=255)
                                       aTACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTa                                                                                                                                          <  4:313465/115‑1 (MQ=255)
                                       aTACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTg                                                                                                        >  5:259983/1‑149 (MQ=255)
                                        tACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGt                                                                                                       <  7:131421/149‑1 (MQ=255)
                                         aCAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTa                                                                                                      >  8:125458/1‑149 (MQ=255)
                                           aaCCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCgg                                                                                                                                                   <  5:47857/102‑1 (MQ=255)
                                           aaCCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCgg                                                                                                                                                   >  6:47857/1‑102 (MQ=255)
                                           aaCCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTAcc                                                                                                    >  7:26531/1‑149 (MQ=255)
                                            accaccAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGt                                                                                                           <  3:199514/141‑1 (MQ=255)
                                            accaccAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGt                                                                                                           >  4:199514/1‑141 (MQ=255)
                                                             aGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAAtgtg                                                                                  >  2:258833/1‑149 (MQ=255)
                                                              gTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGc                                                                                 <  5:297313/149‑1 (MQ=255)
                                                                 ccGCAACGGTGGTGACCACGATGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGc                                                                              >  3:338965/1‑149 (MQ=255)
                                                                                ccACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCt                                                               >  3:206405/1‑149 (MQ=255)
                                                                                 cACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCt                                                               >  8:164277/1‑148 (MQ=255)
                                                                                  acgacgATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTaa                                                                        <  6:83982/138‑1 (MQ=255)
                                                                                  acgacgATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTaa                                                                        >  5:83982/1‑138 (MQ=255)
                                                                                   cgacgaTGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGAc                                                            >  2:83417/1‑149 (MQ=255)
                                                                                        aTGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATg                                                       <  5:363375/149‑1 (MQ=255)
                                                                                           aaGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGga                                                     <  1:83417/148‑1 (MQ=255)
                                                                                                aTCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGTAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACtt                                                <  7:198165/148‑1 (MQ=255)
                                                                                                 tCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTa                                              <  3:286231/149‑1 (MQ=255)
                                                                                                   cATGGCTATTCTTGTTCTCTCTTGAAAGGCCTTCGACACATCCCGGCAGGCTGTACCCCGTCTGAATTAAAACAATTTTATTACGTTTGTACCGTTACATCCGTCAATGTGCAGCGTCTAATGAGCACGTGACTTATGGAGACTTTAc                                             <  8:26531/148‑1 (MQ=255)
                                                                                                   cATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATTAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCGAATGAGCACCTGACTTATGGAGACTTTAcc                                            >  2:292110/1‑149 (MQ=255)
                                                                                                   cATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTAc                                             <  1:258833/148‑1 (MQ=255)
                                                                                                        cTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGc                                       <  4:206405/149‑1 (MQ=255)
                                                                                                         tATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGTTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCg                                      >  6:2845/1‑149 (MQ=255)
                                                                                                              ttGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAAt                                 >  7:238325/1‑149 (MQ=255)
                                                                                                                ggTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAAt                                 <  6:259983/147‑1 (MQ=255)
                                                                                                                  tctcACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCAcc                                                                >  8:28579/1‑114 (MQ=255)
                                                                                                                  tctcACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCAcc                                                                <  7:28579/114‑1 (MQ=255)
                                                                                                                  tctcACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGc                             >  1:91205/1‑149 (MQ=255)
                                                                                                                      aCTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTg                         >  1:345467/1‑149 (MQ=255)
                                                                                                                      aCTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTg                         >  6:302100/1‑149 (MQ=255)
                                                                                                                       cTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCAt                                         <  8:191051/132‑1 (MQ=255)
                                                                                                                       cTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCAt                                         >  7:191051/1‑132 (MQ=255)
                                                                                                                            aaGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtggtg                   <  4:338965/149‑1 (MQ=255)
                                                                                                                            aaGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtggtg                   <  7:125458/149‑1 (MQ=255)
                                                                                                                            aaGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCtggtg                   <  5:302100/149‑1 (MQ=255)
                                                                                                                              ggCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGc                 >  7:299788/1‑149 (MQ=255)
                                                                                                                                  ttCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGAcg             >  6:181373/1‑148 (MQ=255)
                                                                                                                                  ttCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGAcc             >  4:273927/1‑149 (MQ=255)
                                                                                                                                   tCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCt            >  3:251177/1‑149 (MQ=255)
                                                                                                                                   ggggtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCt            >  7:206896/6‑149 (MQ=255)
                                                                                                                                     ggtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCtt           >  6:175097/4‑148 (MQ=255)
                                                                                                                                     gACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCtt           >  4:37653/1‑148 (MQ=255)
                                                                                                                                      gtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGga                                                     <  6:177067/103‑1 (MQ=255)
                                                                                                                                      gtacaGCCTGCCGGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGga                                                     >  5:177067/3‑105 (MQ=255)
                                                                                                                                       cacaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATgg                                                      <  4:352603/103‑1 (MQ=255)
                                                                                                                                       cacaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATgg                                                      >  3:352603/1‑103 (MQ=255)
                                                                                                                                        acaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCttt          >  2:32213/1‑146 (MQ=255)
                                                                                                                                        acaGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCttt          <  1:32213/146‑1 (MQ=255)
                                                                                                                                         caGCCCGGCAGGTTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacca      <  5:2845/149‑1 (MQ=255)
                                                                                                                                         caGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTacca      <  7:164277/149‑1 (MQ=255)
                                                                                                                                           gCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTaccacc    <  8:299788/149‑1 (MQ=255)
                                                                                                                                             ccGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTACCACCAt  >  5:116390/1‑149 (MQ=255)
                                                                                                                                              |                                                                                                                                                   
CAACGCCAGGTCGCGGTCCATCCAGCGGCGCATGCGCACATACAACCACCAGTGGAAATACAGTCCCGCAACGGTGGTGACCACGACGATGAAGTAATCCATGGCTATTCTTGGTCTCACTTGAAAGGCCTTCGACACAGCCCGGCAGGCTGTACCCCGGCTGAATGAAAACAATTTTATTACGTTTGTACCGTGACAGCCGTCAATGTGCAGCGTCTAATGAGCACCTGACTTATGGAGACTTTACCCATGCGTAAATCCGCTTTGCTGGTGGCGACCTTTACCACCAT  >  NC_002947/1294271‑1294560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑