Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 95,387 A→G 6.5% M15V (ATG→GTG)  PP_0087 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294795,3870AG 6.5% 229.8 / 4.0 77M15V (ATG→GTG) PP_0087hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (33/39);  new base G (3/2);  total (36/41)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACTTCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTGGAGATCGTCA  >  NC_002947/95240‑95531
                                                                                                                                                   |                                                                                                                                                 
aCTTCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCCTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGtgat                                                                                                                                                  <  5:295059/149‑1 (MQ=255)
aCTTACAGGTCACTCTGAATGTCGTCTTCCGTATCGCCAATTGCTGATACTACGTTGGTTTGTATGATCCCGGCGTGATGTGGTATTCAGAAATGGGGCGCCTTGGTGGGTCCCCTTCTTATTTCATCTGCACAAGGAACGCGAGtgat                                                                                                                                                  <  3:49537/149‑1 (MQ=255)
 cTTCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGtgat                                                                                                                                                  >  6:142625/1‑148 (MQ=255)
  ttCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGtgatg                                                                                                                                                 >  2:217239/1‑148 (MQ=255)
  ttCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGtgatg                                                                                                                                                 >  2:213819/1‑148 (MQ=255)
    ccAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGATGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGaa                                                                                                                                               >  7:311714/1‑148 (MQ=255)
           aCGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGTGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGa                                                                                                                                       >  4:233351/1‑149 (MQ=255)
             gCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGAtt                                                                                                                                     >  3:45457/1‑149 (MQ=255)
                  aGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGgc                                                                                                                                <  5:55171/149‑1 (MQ=255)
                        gCTTCCGTATCGCCAATAGCTGAAACAACGTTCGGTTGTATGATCCCGGCGTGATGAGGTATACAGAAAAGCGGCGCCTTGGTGGGCCCCCTTCTGATTTCATCTCCACAACGAACGCGACAGATGAACAAACTGATTCTGGCGCTggg                                                                                                                          >  6:369342/1‑149 (MQ=255)
                         cTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGc                                                                                                                         <  4:64601/149‑1 (MQ=255)
                          atCCGTATCGCCAATAGCTGAACCAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGcc                                                                                                                        <  8:64931/148‑1 (MQ=255)
                             cGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCtg                                                                                                                      <  8:311714/148‑1 (MQ=255)
                                    ccAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCt                                                                                                              <  6:54041/149‑1 (MQ=255)
                                           cTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggt                                                                                                       <  1:187567/149‑1 (MQ=255)
                                            tGAAACTAGGTTGGGTTGTATGATCCCGGGGTGATGAGGTATTCTGATAATGGTCGGCTTTGTGGGGCCCCTTCTGATTTCATCTGCACAAGGATCGCGAGTGATGATCAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggtg                                                                                                      <  5:369342/149‑1 (MQ=255)
                                            tGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGGTGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCg                                                                                                         >  1:350500/1‑146 (MQ=255)
                                            tGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGGTGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCg                                                                                                         <  2:350500/146‑1 (MQ=255)
                                            tGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggtg                                                                                                      >  2:152197/1‑149 (MQ=255)
                                            tGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggtg                                                                                                      >  8:7165/1‑149 (MQ=255)
                                             gAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggtgg                                                                                                     >  3:50861/1‑149 (MQ=255)
                                             gAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCggggg                                                                                                     >  1:310314/1‑149 (MQ=255)
                                             gAAACAACGTTGGGTGGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTTATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGTCCTGATGAACCTGGACgttgg                                                                                                     >  2:303349/1‑149 (MQ=255)
                                                 caacGTTGGGTTGTATGATCCCGGGGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAGCTGATTCTGGCGCTGGGACTGATGACGCTGGCCGGTGGTgcg                                                                                                 >  6:54610/1‑149 (MQ=255)
                                                     gTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTgg                                                                                             <  3:112561/149‑1 (MQ=255)
                                                           ttGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCgg                                                                                       >  4:167201/1‑149 (MQ=255)
                                                            tGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGGTGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCgg                                                                                       <  7:126267/148‑1 (MQ=255)
                                                             gTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCggg                                                                                      <  4:45457/148‑1 (MQ=255)
                                                              tATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGGTGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCgg                                                                                       >  8:126267/1‑146 (MQ=255)
                                                               aTGATCCCGGCGAGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCa                                                                                    >  5:275919/1‑148 (MQ=255)
                                                                tGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCaa                                                                                   <  2:310314/148‑1 (MQ=255)
                                                                 gATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAAc                                                                                 >  2:318992/1‑149 (MQ=255)
                                                                 gATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAAc                                                                                 >  3:159875/1‑149 (MQ=255)
                                                                  aTCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAAcc                                                                                <  2:339581/149‑1 (MQ=255)
                                                                   tCCCGGCGTGATGAGGTATTCAGAAAAGGGGAGCCTTGGTGGGGCCACTTCTGATTACATCTCCACAAGGAACGCGACTCATGAACAAACTGATTCTCGAGCTGGGCATGATGACCCTGGCCCGAGGTCCGCAGGCAGCTGGCAAACCg                                                                               >  3:342306/1‑149 (MQ=255)
                                                                     ccGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGt                                                                              <  7:7165/148‑1 (MQ=255)
                                                                     ccGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGt                                                                              <  1:152197/148‑1 (MQ=255)
                                                                     ccGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTg                                                                             >  4:348361/1‑149 (MQ=255)
                                                                         cGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAg                                                                         <  5:133619/149‑1 (MQ=255)
                                                                            gatgaGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGaa                                                                      >  2:206477/1‑149 (MQ=255)
                                                                              tgaGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACt                                                                    >  5:224049/1‑149 (MQ=255)
                                                                                aGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGc                                                                                                           <  8:114045/108‑1 (MQ=255)
                                                                                aGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGc                                                                                                           >  7:114045/1‑108 (MQ=255)
                                                                                aGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCTGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGa                                                                  <  1:213819/149‑1 (MQ=255)
                                                                                 ggTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGa                                                                  <  6:224049/148‑1 (MQ=255)
                                                                                  gTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTATGATTTGATCTGCACAAGGAACGAGAGTGGTGAACATACTGATTATGGCGCTGGGCCTGATGACGCTGGCCGGTGGTGCGATGGCAGCGGGCAAAGCGTGGGAGGAACTGAgg                                                                >  5:333508/1‑149 (MQ=255)
                                                                                     ttCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGc                                                              <  4:50861/148‑1 (MQ=255)
                                                                                     ttCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCg                                                             >  7:187992/1‑149 (MQ=255)
                                                                                     ttCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCg                                                             >  8:87035/1‑149 (MQ=255)
                                                                                         gAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGc                                                                            >  5:320452/1‑130 (MQ=255)
                                                                                         gAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGc                                                                            <  6:320452/130‑1 (MQ=255)
                                                                                            aaGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCACTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTg                                                      <  5:142625/149‑1 (MQ=255)
                                                                                             aGGTGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑c                                                    <  3:233351/149‑1 (MQ=255)
                                                                                              ggggCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑Cg                                                   <  1:318992/149‑1 (MQ=255)
                                                                                              ggggCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑Cg                                                   <  7:122768/149‑1 (MQ=255)
                                                                                              ggggCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGACGGCGGAAATTG‑Cg                                                   <  6:275919/149‑1 (MQ=255)
                                                                                                  cgcCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGcc                                                >  5:288555/1‑148 (MQ=255)
                                                                                                         gtggGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGa                                        >  3:43462/1‑149 (MQ=255)
                                                                                                          tggGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGAt                                       <  4:43462/149‑1 (MQ=255)
                                                                                                          tggGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGAt                                       <  6:288555/149‑1 (MQ=255)
                                                                                                            gggCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGc                                     <  1:260207/149‑1 (MQ=255)
                                                                                                                   ttCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCACTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGc                              >  2:161707/1‑149 (MQ=255)
                                                                                                                    tCTGATGTCATCTGCACAAGGACCGCGAGTGATGAACAAACTGATGCTGGCGCTGGGCCTGATGGCCCTGGCCGGTGGGGCGCTGGCGGGGGGCACGGCGGGGGCGGGGCGGAGGGCGGAGATTG‑CGGCCACGCTGGATGCCATGGGCg                             >  4:88891/1‑149 (MQ=255)
                                                                                                                     cTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATg                                      >  3:47484/1‑139 (MQ=255)
                                                                                                                     cTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATg                                      <  4:47484/139‑1 (MQ=255)
                                                                                                                       gATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCa                          >  7:162228/1‑149 (MQ=255)
                                                                                                                        aTTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCAc                         >  5:45175/1‑149 (MQ=255)
                                                                                                                           tCATCTGCTCAAGGAACGCTAGTGATGAAGAAACTGATTCTGGCGCTGGGCCTGATGACCGTGGCCGGTGGTGCGCTGGCAGAGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTg                       <  4:342306/148‑1 (MQ=255)
                                                                                                                           tCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTg                       <  1:206477/148‑1 (MQ=255)
                                                                                                                             aTCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGc                     >  3:10210/1‑148 (MQ=255)
                                                                                                                                tGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTGACGGCCAAGCTGGATGCCAAGGGCGTCACt                        <  1:209979/143‑1 (MQ=255)
                                                                                                                                tGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACt                        >  2:209979/1‑142 (MQ=255)
                                                                                                                                tGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTAc                  >  1:348517/1‑148 (MQ=255)
                                                                                                                                    caAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGt                            <  5:229019/134‑1 (MQ=255)
                                                                                                                                    caAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGt                            >  6:229019/1‑134 (MQ=255)
                                                                                                                                     aacgAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTg            <  8:291495/146‑1 (MQ=255)
                                                                                                                                     aaGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAgg                                <  6:82005/129‑1 (MQ=255)
                                                                                                                                     aaGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTg            <  2:42407/149‑1 (MQ=255)
                                                                                                                                     aaGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTg            <  7:87035/149‑1 (MQ=255)
                                                                                                                                              gAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTGGTGATCGTc   <  4:159875/149‑1 (MQ=255)
                                                                                                                                              gAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTGGAGATCGTc   <  3:167201/149‑1 (MQ=255)
                                                                                                                                               aGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTGGAGATCGTCa  <  1:217239/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
ACTTCCAGGTCACGCTGAAGGTCGGCTTCCGTATCGCCAATAGCTGAAACAACGTTGGGTTGTATGATCCCGGCGTGATGAGGTATTCAGAAAAGGGGCGCCTTGGTGGGGCCCCTTCTGATTTCATCTGCACAAGGAACGCGAGTGATGAACAAACTGATTCTGGCGCTGGGCCTGATGACCCTGGCCGGTGGTGCGCTGGCAGCGGGCAAACCGTGCGAGGAACTGAGGGCGGAAATTG‑CGGCCAAGCTGGATGCCAAGGGCGTCACTGGCTACAAGCTGGAGATCGTCA  >  NC_002947/95240‑95531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑