Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,316,612 G→A 8.1% A10A (GCG→GCA)  PP_1150 → membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,316,6120GA 8.1% 134.8 / 2.1 49A10A (GCG→GCA) PP_1150membrane protein
Reads supporting (aligned to +/- strand):  ref base G (23/22);  new base A (2/2);  total (25/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.93e-01

CCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTCATCGTCGCCCAGCCGCTATCGGGC  >  NC_002947/1316465‑1316753
                                                                                                                                                    |                                                                                                                                             
ccTTTCAGTGCAGGCCAATCGAATATAAATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCgcgc                                                                                                                                              >  3:51391/1‑149 (MQ=255)
       gTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCACCcagcag                                                                                                                                       >  1:187521/1‑149 (MQ=255)
       gTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCACCcagcag                                                                                                                                       >  3:93733/1‑149 (MQ=255)
              ccAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGcc                                                                                                                                <  6:284679/149‑1 (MQ=255)
                aaTCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCt                                                                                                                              >  2:252291/1‑149 (MQ=255)
                 aTCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTc                                                                                                                             <  4:232451/149‑1 (MQ=255)
                 aTCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTc                                                                                                                             <  4:51391/149‑1 (MQ=255)
                    gAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAg                                                                                                                          >  6:270870/1‑149 (MQ=255)
                      atatACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGt                                                                                                                         <  6:243314/148‑1 (MQ=255)
                          aCATGATTAGCTAGCTAATTAATCGTTTCGCGCTTTATCCTGCTCATCATTGTCTATCTTTTAAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGACCTGTTCGAGCCca                                                                                                                                           >  1:26962/1‑127 (MQ=255)
                          aCATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCca                                                                                                                                           <  2:26962/126‑1 (MQ=255)
                               aTTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCaa                                                                                                               <  5:270870/149‑1 (MQ=255)
                                     tagctaATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCagcagc                                                                                                                                      >  1:139114/1‑120 (MQ=255)
                                     tagctaATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCagcagc                                                                                                                                      <  2:139114/120‑1 (MQ=255)
                                          aattaatCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCg                                                                                                    >  7:255799/1‑149 (MQ=255)
                                             taatCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCggg                                                                                                  >  4:292204/1‑148 (MQ=255)
                                             taatCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCggg                                                                                                  <  3:292204/148‑1 (MQ=255)
                                              aatCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCggggg                                                                                                >  4:330693/1‑149 (MQ=255)
                                               atCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGggc                                                                                               <  3:330693/149‑1 (MQ=255)
                                                      c‑cGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATAAAGACCCTGCTCGGGGGCGGCCTgg                                                                                        >  8:107222/1‑149 (MQ=255)
                                                         gCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCg                                                                                      >  4:186369/1‑149 (MQ=255)
                                                             tATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTtgt                                                                                  >  5:142752/1‑149 (MQ=255)
                                                               tCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtgg                                                                                >  8:70258/1‑149 (MQ=255)
                                                                    cTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctggc                                                                           <  4:8285/149‑1 (MQ=255)
                                                                    cTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCACCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctg                                                                             <  2:187521/147‑1 (MQ=255)
                                                                    cTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCACCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGtggctg                                                                             <  4:93733/147‑1 (MQ=255)
                                                                       atcatTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGAt                                                                        >  3:120232/1‑149 (MQ=255)
                                                                        tcatTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATg                                                                       >  3:316675/1‑149 (MQ=255)
                                                                             gTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGtt                                                                                    <  8:195809/131‑1 (MQ=255)
                                                                             gTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGtt                                                                                    >  7:195809/1‑131 (MQ=255)
                                                                             gTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTg                                                                  >  6:135062/1‑149 (MQ=255)
                                                                              tCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTgg                                                                 <  1:252291/149‑1 (MQ=255)
                                                                              tCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTg                                                                  <  4:316675/148‑1 (MQ=255)
                                                                                    ttttCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTg                                                           <  6:142752/149‑1 (MQ=255)
                                                                                       tCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAg                                                        >  1:39634/1‑149 (MQ=255)
                                                                                          gCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCACTGGGGGCTGGAGCaa                                                     <  8:255799/149‑1 (MQ=255)
                                                                                             aGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAAccc                                                  >  7:265308/1‑149 (MQ=255)
                                                                                                 ttttCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACcctcct                                              >  5:115113/1‑149 (MQ=255)
                                                                                                   ttCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACcctcct                                              <  2:39634/147‑1 (MQ=255)
                                                                                                       ccGCAAGGTCCGCCTCGTGCCCATCACCATTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGATTGCCATCAAGACCCTGCTCGGGGGCGGCATGGAGGTGTGGCTGGCGATGCGCTGGGGGCTGTAGCAACCCTCATGGGCAt                                        >  1:203993/1‑149 (MQ=255)
                                                                                                        cGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCAtt                                       >  2:282138/1‑149 (MQ=255)
                                                                                                         gCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATtg                                      <  7:107222/149‑1 (MQ=255)
                                                                                                          cAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATtga                                     >  5:63196/1‑149 (MQ=255)
                                                                                                             ggTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATtgatga                                  <  3:186369/149‑1 (MQ=255)
                                                                                                                       gTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTc                          <  5:135062/147‑1 (MQ=255)
                                                                                                                       gTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTCa                         <  7:70258/148‑1 (MQ=255)
                                                                                                                           ccttCACCTTTCAGGGCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGTCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTCAtcgtc                    <  2:203993/146‑1 (MQ=255)
                                                                                                                             aTCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACCGCTTTCATCGTCGc                  >  4:285418/1‑149 (MQ=255)
                                                                                                                              tCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTCATCGTCGcc                 >  3:170201/1‑149 (MQ=255)
                                                                                                                                               ttCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACGGCTTTCATCGTCGCCCAGCCGCTATCGGGc  <  3:285418/147‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
CCTTTCAGTGCAGGCCAATCGAATATACATGATTAGCTAGCTAATTAATCGTTTC‑CGCTTTATCCTGCTCATCATTGTCTATCTTTTCAGCAAGACTTTTCCCCGCAAGGTCCGCCTCGTGCCCATCACCTTTCAGGCCCTGTTCGCGCCCAGCAGCCTCGCCCTCAAGTTTGCCATCAAGACCCTGCTCGGGGGCGGCCTGGCGTTGTGGCTGGCGATGCGCTGGGGGCTGGAGCAACCCTCCTGGGCATTGATGACTGCTTTCATCGTCGCCCAGCCGCTATCGGGC  >  NC_002947/1316465‑1316753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑