Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,368,197 G→T 6.2% intergenic (+26/‑157) PP_1193 → / → PP_1194 membrane protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,368,1970GT 6.2% 203.6 / 3.3 64intergenic (+26/‑157)PP_1193/PP_1194membrane protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (35/25);  new base T (2/2);  total (37/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.49e-01

TGTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTTATACTGGGTGCCT  >  NC_002947/1368050‑1368343
                                                                                                                                                   |                                                                                                                                                  
tGTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGc                                                                                                                                                   >  4:202239/1‑149 (MQ=255)
    ggtggtGAAGCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACTGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGTCCCATACAAAACCCAGCGGa                                                                                                                                                <  2:356415/148‑1 (MQ=255)
            aCCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGtttt                                                                                                                                        >  3:91455/1‑148 (MQ=255)
            aCCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGtttt                                                                                                                                        <  4:91455/148‑1 (MQ=255)
             ccTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTg                                                                                                                                       >  3:106353/1‑148 (MQ=255)
             ccTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCAAGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTg                                                                                                                                       >  1:88506/1‑148 (MQ=255)
              cTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTa                                                                                                                                     <  3:336927/149‑1 (MQ=255)
               tGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTAt                                                                                                                                    >  6:9817/1‑149 (MQ=255)
                      gATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAgg                                                                                                                             >  4:181515/1‑149 (MQ=255)
                         cACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCGTGCGCTGGGTTTTGTATg                                                                                                                                   <  4:146142/140‑1 (MQ=255)
                         cACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCGTCCGCTGGGTTTTGTATg                                                                                                                                   >  3:146142/1‑140 (MQ=255)
                          aCGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCt                                                                                                                         >  2:210378/1‑149 (MQ=255)
                           cGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAACCCCAGCGGACGGGGTTTGTATGAGCAGGTTCTg                                                                                                                        >  4:13461/1‑149 (MQ=255)
                           cGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTg                                                                                                                        <  5:9817/149‑1 (MQ=255)
                            ggCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGtttt                                                                                                                                        >  3:136095/1‑132 (MQ=255)
                            ggCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGtttt                                                                                                                                        <  4:136095/132‑1 (MQ=255)
                             gCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGc                                                                                                                      <  2:170451/149‑1 (MQ=255)
                              cAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGTTTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAgg                                                                                                                             >  1:14205/1‑141 (MQ=255)
                              cAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGTTTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAgg                                                                                                                             <  2:14205/141‑1 (MQ=255)
                                     gtgggtggCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTg                                                                                                              >  5:79298/1‑149 (MQ=255)
                                      tgggtggCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGc                                                                                                             <  1:210378/149‑1 (MQ=255)
                                        ggtggCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCt                                                                                                           >  5:103566/1‑149 (MQ=255)
                                          tggCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCttt                                                                                                         <  4:106353/149‑1 (MQ=255)
                                          tggCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCttt                                                                                                         <  2:88506/149‑1 (MQ=255)
                                              gAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCa                                                                                                     >  3:351276/1‑149 (MQ=255)
                                                aCCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGACTTTATCACg                                                                                                   >  6:171732/1‑149 (MQ=255)
                                                  ccGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTc                                                                                                 <  6:103566/149‑1 (MQ=255)
                                                           aCCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAAt                                                                                        <  3:202239/149‑1 (MQ=255)
                                                                 gtgtTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAgcgc                                                                                  <  6:79298/149‑1 (MQ=255)
                                                                    ttCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGa                                                                               <  5:257125/149‑1 (MQ=255)
                                                                           cggcggCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTg                                                                                                                        >  4:336110/1‑101 (MQ=255)
                                                                              cggcgAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTc                                                                     <  3:181515/149‑1 (MQ=255)
                                                                                     gCCGGGGATGACAACAGCAGAAGCTGATTGGCACTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTc                                                               >  1:350171/1‑148 (MQ=255)
                                                                                       cGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCAt                                                            <  5:171732/149‑1 (MQ=255)
                                                                                          ggATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCCTTTTCCTCGTc                                                               <  8:94762/143‑1 (MQ=255)
                                                                                          ggATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCCTTTTCCTCGTc                                                               >  7:94762/1‑143 (MQ=255)
                                                                                             tGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCggg                                                      <  4:351276/149‑1 (MQ=255)
                                                                                              gACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGa                                                     >  1:140081/1‑149 (MQ=255)
                                                                                                caacaGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGata                                                   >  2:138019/1‑149 (MQ=255)
                                                                                                caacaGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGata                                                   >  5:39656/1‑149 (MQ=255)
                                                                                                     gcagAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCAAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCAGGGATATGAtt                                              >  7:264983/1‑149 (MQ=255)
                                                                                                       agAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGc                                            >  7:59360/1‑149 (MQ=255)
                                                                                                           gCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGa                                                <  4:345263/141‑1 (MQ=255)
                                                                                                           gCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGa                                                >  3:345263/1‑141 (MQ=255)
                                                                                                                     gcTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGAt                              <  8:59360/149‑1 (MQ=255)
                                                                                                                     gcTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGAt                              >  8:220036/1‑149 (MQ=255)
                                                                                                                       tGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGTGTGGGAtt                             >  5:127879/1‑148 (MQ=255)
                                                                                                                         aCCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTg                                      <  4:140918/137‑1 (MQ=255)
                                                                                                                         aCCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTg                                      >  3:140918/1‑137 (MQ=255)
                                                                                                                           ccGAAGTGGCACATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGATTGCGTGGGATTAATTc                        >  7:346027/1‑149 (MQ=255)
                                                                                                                             gAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCGATGCCGGGATATGATTGCGGTTTGCGTGGGATTTATTccc                      >  8:78303/1‑149 (MQ=255)
                                                                                                                               aGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCcgc                    >  5:59931/1‑149 (MQ=255)
                                                                                                                                   gCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTc                 <  2:140081/148‑1 (MQ=255)
                                                                                                                                   gCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCt                <  2:350171/149‑1 (MQ=255)
                                                                                                                                    ctcATACAAAACCCGTCCGCTGGGTTTTGTATGGGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTgg                                 <  3:342683/129‑1 (MQ=38)
                                                                                                                                    ctcATACAAAACCCGTCCGCTGGGTTTTGTATGGGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTgg                                 >  4:342683/3‑131 (MQ=38)
                                                                                                                                     ccATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTta              >  3:153362/1‑149 (MQ=255)
                                                                                                                                     ccATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTta              >  3:298939/1‑149 (MQ=255)
                                                                                                                                     ccATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTta              >  4:176501/1‑149 (MQ=255)
                                                                                                                                       aTACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTtata            >  3:123429/1‑149 (MQ=255)
                                                                                                                                        tACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTTATAc           >  3:291400/1‑149 (MQ=255)
                                                                                                                                         aCAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCcgc                    <  3:82656/139‑1 (MQ=255)
                                                                                                                                         aCAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCcgc                    >  4:82656/1‑139 (MQ=255)
                                                                                                                                                 cAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTTATACTGGGTGCct  <  8:346027/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TGTTGGTGGTGAACCTGGTGACGATCACGGCAACCATGTGGGTGGCGAACCCGGTAATGACCACGGTGTTCATGCCGGCGGCGAGGCCGGGGATGACAACAGCAGAAGCTGATTGGCGCTGACCCGAAGTGGCCCATACAAAACCCAGCGGACGGGTTTTGTATGAGCAGGTTCTGGCCTGTCTTGCCTTTATCACGTCATTTCCAATGAGCGCTGACAGCTTTTTCCTCGTCCATGCCGGGATATGATTGCGGTTTGCGTGGGATTAATTCCCGCGTCTTATACTGGGTGCCT  >  NC_002947/1368050‑1368343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑