| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 126,115 | A→G | 6.7% | intergenic (+25/+25) | PP_0120 → / ← thrB | zinc ABC transporter/homoserine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 126,115 | 0 | A | G | 6.7% | 168.4 / 2.1 | 59 | intergenic (+25/+25) | PP_0120/thrB | zinc ABC transporter/homoserine kinase |
| Reads supporting (aligned to +/- strand): ref base A (28/27); new base G (2/2); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.08e-01 | |||||||||||
TACGCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGGCGCGAC > NC_002947/125975‑126263 | tACGCCTTGCCGAGCTGGATGCCTTGGGGGGCACCGAGCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCTGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCcgcg < 5:261292/149‑1 (MQ=255) ccGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAg < 3:92371/149‑1 (MQ=255) gAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGAc > 6:142606/1‑149 (MQ=255) gATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGt < 6:208715/148‑1 (MQ=255) gTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAgcgc < 6:53511/147‑1 (MQ=255) gTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAgcgc > 5:53511/1‑147 (MQ=255) gTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGcgcg > 8:6860/1‑148 (MQ=255) tGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGaa < 7:6860/149‑1 (MQ=255) gCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCcgc > 1:213829/1‑118 (MQ=255) gCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCcgc < 2:213829/118‑1 (MQ=255) aCCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACgg > 2:177807/1‑148 (MQ=255) tCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGt < 8:298349/148‑1 (MQ=255) ggTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCGGACGCGGTCTACAGCGCGAACGGCAGGTGCaa < 1:213547/149‑1 (MQ=255) tGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCGTGCCCGCgaaggg > 6:223157/1‑106 (MQ=255) tGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCGTGCCCGCgaaggg < 5:223157/108‑3 (MQ=255) tGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCAc > 3:73760/1‑149 (MQ=255) gCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAAt < 2:152343/142‑1 (MQ=255) gCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAAt > 1:152343/1‑142 (MQ=255) cAAAGGGTATGAGCGCTTGCTGGAGAAGTTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACctg > 8:228046/1‑149 (MQ=255) taGGATATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCAc < 6:246572/143‑1 (MQ=255) aaGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTAGCGGGCACGCCCGCGAAGAAGCAGACCCGGTCTAAAGCGCGAACGGCAGGTGCAATGCCAccagaa > 5:246572/1‑145 (MQ=255) gggTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCGTGCCcgcg > 1:148574/1‑96 (MQ=255) gggTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCGTGCCcgcg < 2:148574/96‑1 (MQ=255) ggTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGgc > 7:264805/1‑149 (MQ=255) tGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTg > 4:68522/1‑149 (MQ=255) gcgcTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCTTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc < 1:119363/149‑1 (MQ=255) gcgcTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc < 1:177807/149‑1 (MQ=255) cgcTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc > 3:128036/1‑148 (MQ=255) cgcTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTgcgc > 8:46723/1‑148 (MQ=255) ttGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAg > 1:57829/1‑148 (MQ=255) ttGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGa > 8:149488/1‑149 (MQ=255) gCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAg < 2:57829/146‑1 (MQ=255) ggAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCa > 2:110389/1‑148 (MQ=255) agaAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACtt > 8:184788/1‑149 (MQ=255) gaAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTc < 8:264805/149‑1 (MQ=255) aaGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTc > 6:45418/1‑148 (MQ=255) gCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGaa < 7:149488/149‑1 (MQ=255) cTGGTCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGc < 8:146211/104‑1 (MQ=255) cTGGGCGGTGACCTGGCAGGCTGACTGGAGCAGTTGTAAGACTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGc > 7:146211/1‑104 (MQ=255) ggTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAActcgctc > 2:238460/1‑149 (MQ=255) cTGGCAGGCTGCCTGGATCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACTCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTg < 2:15578/97‑1 (MQ=255) cTGGCAGGCTGCCTGGAGCAGTTGTAAACCTGTGCCGGCCCCTACGCGGGCACGCCAGCGAAGAAGCAGACGCAGTCTACAGCGCGAACGGCAGGTg > 1:15578/1‑97 (MQ=255) gCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGgcg < 6:86825/108‑1 (MQ=255) gCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGgcg > 5:86825/1‑108 (MQ=255) ccTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGatcatc < 4:128036/147‑1 (MQ=255) tAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGc > 1:235861/1‑148 (MQ=255) gCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAAc < 7:46723/149‑1 (MQ=255) ccTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACctg > 7:171779/1‑81 (MQ=255) ccTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACctg < 8:171779/81‑1 (MQ=255) tgtgCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCCTCCCGGCGAACGAc > 1:257365/1‑149 (MQ=255) tgCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGAc < 7:184788/147‑1 (MQ=255) gCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCg < 5:142606/149‑1 (MQ=255) cccTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAAc > 3:333230/1‑98 (MQ=255) cccTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAAc < 4:333230/98‑1 (MQ=255) cTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAAc > 8:207197/1‑135 (MQ=255) cTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAAc < 7:207197/135‑1 (MQ=255) gcgGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAg < 5:45418/148‑1 (MQ=255) gggCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGgcg > 2:257987/1‑149 (MQ=255) ggCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGgcgc < 1:110389/149‑1 (MQ=255) aCGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGGCGCGAc < 4:73760/149‑1 (MQ=255) | TACGCCTTGCCGAGCTGGATGCCTTGGGTGGCACCGATCCGGTGGATGGCAAAGGGTATGAGCGCTTGCTGGAGAAGCTGGGCGGTGACCTGGCAGGCTGCCTGGAGCAGTTGTAAGCCTGTGCCGGCCCCTTCGCGGGCACGCCCGCGAAGAAGCAGACGCGGTCTACAGCGCGAACGGCAGGTGCAATGCCACCTGCTGGCGCTGCGCCAGACGCACTTCGAACTCGCTCGGATCGTGGATCATCACATCCATCCCGGCGAACGACTCGGCCGCAATCAGGCGCGAC > NC_002947/125975‑126263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |