Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,428,975 A→G 15.4% Y359C (TAC→TGC) ‡ mqo‑II → malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,428,9750AG15.4% 86.3 / 8.6 39Y359C (TAC→TGC) ‡mqo‑IImalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (16/17);  new base G (2/4);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01

GTGTGCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGA  >  NC_002947/1428828‑1429119
                                                                                                                                                   |                                                                                                                                                
gtgtGCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTAc                                                                                                                                                 <  1:340252/149‑1 (MQ=255)
      tGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTg                                                                                                                                           >  3:232297/1‑149 (MQ=255)
        gACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGt                                                                                                                                         >  3:162712/1‑149 (MQ=255)
           ggCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGa                                                                                                                                      <  1:156667/149‑1 (MQ=255)
              aaGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGatac                                                                                                                                   <  3:172521/149‑4 (MQ=39)
                 cgcgTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCt                                                                                                                                <  1:301408/149‑1 (MQ=255)
                                          cTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGAc                                                                                                       <  2:61463/149‑1 (MQ=255)
                                          cTACCTTCTCGACCAACTTCCTGAAGAACGGCTCGTACCTGGAGCTGCTGTGGAGCACCACCACCCACTACTTGTGGCCGTTTTCAAAAGTCGGTATCGAGCAGTACCCACTGGTGGAGTACCTGGCTGGCCAGCTTATTCTGTCTGAc                                                                                                       <  4:163865/149‑1 (MQ=255)
                                             ccTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGAt                                                                                                    >  7:93269/1‑149 (MQ=255)
                                                    gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCt                                                                                             <  4:162712/149‑1 (MQ=255)
                                                               tGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGTTGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCGGACGATGACCGCTTCGAAGACCTg                                                                                  >  6:22092/1‑149 (MQ=255)
                                                               tGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCa                                                                                                                       <  6:49242/112‑1 (MQ=255)
                                                                gaagaaCGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTgc                                                                                 >  6:299762/1‑149 (MQ=255)
                                                                               ccTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCg                                                                   >  5:346626/1‑148 (MQ=255)
                                                                                  ggACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACg                                                               >  6:15729/1‑149 (MQ=255)
                                                                                            agcagcACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag                                                      <  4:232297/148‑1 (MQ=255)
                                                                                                 caccaccacCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAAt                                                <  8:93269/149‑1 (MQ=255)
                                                                                                  accaccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                               >  8:334865/1‑149 (MQ=255)
                                                                                                        acccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCt                                         <  6:346626/149‑1 (MQ=255)
                                                                                                          ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                               <  4:92867/141‑1 (MQ=255)
                                                                                                          ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                               >  3:92867/1‑141 (MQ=255)
                                                                                                                    aggCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAggccgg                             <  2:273194/148‑1 (MQ=255)
                                                                                                                         gATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgc                        >  8:311455/1‑149 (MQ=255)
                                                                                                                         gATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgc                        <  7:334865/149‑1 (MQ=255)
                                                                                                                           tGACCAAAGTCGGTATCGACCAGTACCCACTTGTCGAGAACCTCGCTGGCCATCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGACCAgcgc                      <  6:315812/149‑1 (MQ=255)
                                                                                                                           tGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgcgc                      <  5:299762/149‑1 (MQ=255)
                                                                                                                               cAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                   >  1:44773/1‑148 (MQ=255)
                                                                                                                                       tactCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcat           >  2:50294/4‑148 (MQ=255)
                                                                                                                                       gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc          <  2:44773/149‑1 (MQ=255)
                                                                                                                                       gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc          <  5:15729/149‑1 (MQ=255)
                                                                                                                                       gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcat           >  6:114724/1‑148 (MQ=255)
                                                                                                                                        tATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc          >  5:111488/1‑148 (MQ=255)
                                                                                                                                         ctCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAAtcatca         <  1:50294/147‑1 (MQ=255)
                                                                                                                                          tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc          >  3:222616/1‑146 (MQ=255)
                                                                                                                                          tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc          <  4:222616/146‑1 (MQ=255)
                                                                                                                                            gACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                        >  6:103843/1‑114 (MQ=255)
                                                                                                                                            gACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                        <  5:103843/114‑1 (MQ=255)
                                                                                                                                             aCCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGt    >  5:217123/1‑149 (MQ=255)
                                                                                                                                               cAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGa  >  7:330038/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTGTGCTGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGA  >  NC_002947/1428828‑1429119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑