Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,428,978 C→T 15.8% P360L (CCA→CTA) ‡ mqo‑II → malate:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,428,9780CT15.8% 86.9 / 8.1 38P360L (CCA→CTA) ‡mqo‑IImalate:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (16/16);  new base T (2/4);  total (18/20)
Fisher's exact test for biased strand distribution p-value = 6.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.45e-01

TGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC  >  NC_002947/1428834‑1429123
                                                                                                                                                |                                                                                                                                                 
tGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTg                                                                                                                                               >  3:232297/1‑149 (MQ=255)
  gACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGt                                                                                                                                             >  3:162712/1‑149 (MQ=255)
     ggCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGa                                                                                                                                          <  1:156667/149‑1 (MQ=255)
        aaGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTGGGTACTGGTCGatac                                                                                                                                       <  3:172521/149‑4 (MQ=39)
           cgcgTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCt                                                                                                                                    <  1:301408/149‑1 (MQ=255)
                                    cTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGAc                                                                                                           <  2:61463/149‑1 (MQ=255)
                                    cTACCTTCTCGACCAACTTCCTGAAGAACGGCTCGTACCTGGAGCTGCTGTGGAGCACCACCACCCACTACTTGTGGCCGTTTTCAAAAGTCGGTATCGAGCAGTACCCACTGGTGGAGTACCTGGCTGGCCAGCTTATTCTGTCTGAc                                                                                                           <  4:163865/149‑1 (MQ=255)
                                       ccTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGAt                                                                                                        >  7:93269/1‑149 (MQ=255)
                                              gACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCt                                                                                                 <  4:162712/149‑1 (MQ=255)
                                                         tGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGTTGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCGGACGATGACCGCTTCGAAGACCTg                                                                                      >  6:22092/1‑149 (MQ=255)
                                                         tGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCa                                                                                                                           <  6:49242/112‑1 (MQ=255)
                                                          gaagaaCGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTgc                                                                                     >  6:299762/1‑149 (MQ=255)
                                                                         ccTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCg                                                                       >  5:346626/1‑148 (MQ=255)
                                                                            ggACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACg                                                                   >  6:15729/1‑149 (MQ=255)
                                                                                      agcagcACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCaagaaag                                                          <  4:232297/148‑1 (MQ=255)
                                                                                           caccaccacCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAAt                                                    <  8:93269/149‑1 (MQ=255)
                                                                                            accaccaccCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                                   >  8:334865/1‑149 (MQ=255)
                                                                                                  acccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCt                                             <  6:346626/149‑1 (MQ=255)
                                                                                                    ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                                   <  4:92867/141‑1 (MQ=255)
                                                                                                    ccacAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATg                                                   >  3:92867/1‑141 (MQ=255)
                                                                                                              aggCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAggccgg                                 <  2:273194/148‑1 (MQ=255)
                                                                                                                   gATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgc                            >  8:311455/1‑149 (MQ=255)
                                                                                                                   gATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgc                            <  7:334865/149‑1 (MQ=255)
                                                                                                                     tGACCAAAGTCGGTATCGACCAGTACCCACTTGTCGAGAACCTCGCTGGCCATCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGACCAgcgc                          <  6:315812/149‑1 (MQ=255)
                                                                                                                     tGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAgcgc                          <  5:299762/149‑1 (MQ=255)
                                                                                                                         cAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTg                       >  1:44773/1‑148 (MQ=255)
                                                                                                                                 tactCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcat               >  2:50294/4‑148 (MQ=255)
                                                                                                                                 gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc              <  2:44773/149‑1 (MQ=255)
                                                                                                                                 gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc              <  5:15729/149‑1 (MQ=255)
                                                                                                                                 gTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcat               >  6:114724/1‑148 (MQ=255)
                                                                                                                                  tATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc              >  5:111488/1‑148 (MQ=255)
                                                                                                                                   ctCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAAtcatca             <  1:50294/147‑1 (MQ=255)
                                                                                                                                    tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc              >  3:222616/1‑146 (MQ=255)
                                                                                                                                    tCGACCAGTGGGTACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAatcatc              <  4:222616/146‑1 (MQ=255)
                                                                                                                                      gACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                            >  6:103843/1‑114 (MQ=255)
                                                                                                                                      gACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCtg                                            <  5:103843/114‑1 (MQ=255)
                                                                                                                                       aCCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGt        >  5:217123/1‑149 (MQ=255)
                                                                                                                                         cAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGa      >  7:330038/1‑149 (MQ=255)
                                                                                                                                               ccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc  >  7:81584/1‑147 (MQ=255)
                                                                                                                                               ccACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGcc  >  8:278065/1‑147 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TGGACGGCAAGCGCGTCATCCTGTTTGGCCCGTTCGCTACCTTCTCGACCAAGTTCCTGAAGAACGGCTCGTACCTGGACCTGCTGAGCAGCACCACCACCCACAACGTGTGGCCGATGACCAAAGTCGGTATCGACCAGTACCCACTGGTCGAGTACCTCGCTGGCCAGCTGATGCTGTCTGACGATGACCGCTTCGAAGCCCTGCGCACCTACTTCCCGAACGCCAAGAAAGAAGAATGGCGCCTGTGGCAGGCCGGCCAGCGCGTGCAAATCATCAAACGTGATGCC  >  NC_002947/1428834‑1429123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑