Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,433,029 T→A 11.3% intergenic (+17/+107) xenA → / ← PP_1255 protein XenA/cis‑4‑hydroxy‑D‑proline oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,433,0290TA11.3% 141.1 / 8.6 53intergenic (+17/+107)xenA/PP_1255protein XenA/cis‑4‑hydroxy‑D‑proline oxidase
Reads supporting (aligned to +/- strand):  ref base T (22/25);  new base A (2/4);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

TGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGCCC  >  NC_002947/1432882‑1433174
                                                                                                                                                   |                                                                                                                                                 
tGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCtt                                                                                                                                                  >  2:268995/1‑149 (MQ=255)
  gTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCttt                                                                                                                                                 <  1:268995/148‑1 (MQ=255)
  gTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCttt                                                                                                                                                 <  7:10111/148‑1 (MQ=255)
   tttCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGc                                                                                                                                               <  5:367602/149‑1 (MQ=255)
      cGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAg                                                                                                                                             <  6:29450/148‑1 (MQ=255)
        gTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCAAAGCAGccc                                                                                                                                          <  4:356033/149‑1 (MQ=255)
             gcgcgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCAAAGCAGcccctcc                                                                                                                                      <  3:254806/148‑5 (MQ=255)
              cgcgcggACGTGGCCGACGCGACCTGGGGTTACTTTGCTGGCTTGGAGTTGGGGGTGGAATAAGCGTGATGGAGCTTGCCGGCGCCTTATTCTCACTGGCTCGAGGGTTATCGCTGATGCTTGGAGGGGCTGCTTTGCAGCCCATTcgc                                                                                                                                    <  5:226535/149‑1 (MQ=255)
                cgcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCgg                                                                                                                                  <  2:152153/149‑1 (MQ=255)
                 gcgcACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCggg                                                                                                                                 >  2:33436/1‑149 (MQ=255)
                               cccGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCca                                                                                                                   >  8:237219/1‑149 (MQ=255)
                                  gCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCATACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCca                                                                                                                   >  8:317345/1‑146 (MQ=255)
                                  gCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGATGGGCTGCAAAGCAGcccctccaa                                                                                                                                    >  8:84383/1‑123 (MQ=255)
                                  gCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGATGGGCTGCAAAGCAGcccctccaa                                                                                                                                    <  7:84383/129‑7 (MQ=255)
                                   cACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACaga                                                                                                               <  7:317163/149‑1 (MQ=255)
                                   cACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACaga                                                                                                               <  3:243835/149‑1 (MQ=255)
                                           ttACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTCCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCg                                                                                                        >  5:311723/1‑148 (MQ=255)
                                             aCTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCTCACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCa                                                                                                                               <  1:192643/123‑1 (MQ=255)
                                             aCTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCCCACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCa                                                                                                                               >  2:192643/1‑123 (MQ=255)
                                                      ccAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg                                                                                                                                                <  7:291470/97‑1 (MQ=255)
                                                      ccAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTg                                                                                                                                                >  8:291470/1‑97 (MQ=255)
                                                      ccAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAg                                                                                              <  6:311723/147‑1 (MQ=255)
                                                      ccAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGa                                                                                             >  7:51711/1‑148 (MQ=255)
                                                       cAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCcac                                                                                                                  >  1:192296/1‑126 (MQ=255)
                                                       cAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCcac                                                                                                                  <  2:192296/126‑1 (MQ=255)
                                                         aGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAgg                                                                                                    <  1:270511/138‑1 (MQ=255)
                                                         aGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAgg                                                                                                    >  2:270511/1‑138 (MQ=255)
                                                               tGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCAccc                                                                                   >  6:193872/1‑149 (MQ=255)
                                                                 ggggTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAg                                                                                                     >  8:197058/1‑129 (MQ=255)
                                                                 ggggTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAg                                                                                                     <  7:197058/129‑1 (MQ=255)
                                                                    gTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCctctct                                                                              >  3:125761/1‑149 (MQ=255)
                                                                     tGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtg                                                                             >  5:281798/1‑149 (MQ=255)
                                                                       gAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATTCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgt                                                                            <  8:51711/148‑1 (MQ=255)
                                                                       gAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg                                                                           >  3:75362/1‑149 (MQ=255)
                                                                       gAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg                                                                           <  4:75362/149‑1 (MQ=255)
                                                                        aaaaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCtgtg                                                                           >  3:198929/1‑148 (MQ=255)
                                                                          aaaGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTgg                                                                          >  3:243026/1‑147 (MQ=255)
                                                                              cctcctTGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTgg                                                                    <  1:363621/149‑1 (MQ=255)
                                                                               ctcctGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTggg                                                                   >  5:1016/1‑149 (MQ=255)
                                                                                              ggCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGCGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATgg                                                    <  7:237219/149‑1 (MQ=255)
                                                                                                      aTGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTa                                             <  6:1016/148‑1 (MQ=255)
                                                                                                         cacaCTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTACACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAgggg                                         >  4:187299/1‑149 (MQ=255)
                                                                                                                    gAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCg                                <  6:281798/147‑1 (MQ=255)
                                                                                                                    gAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCg                                <  1:33436/147‑1 (MQ=255)
                                                                                                                      gCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTgg                                                                    >  4:316136/1‑109 (MQ=255)
                                                                                                                      gCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTACACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTc                            <  3:187299/149‑1 (MQ=255)
                                                                                                                            tCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAgaga                                                                                                             <  6:274528/62‑1 (MQ=255)
                                                                                                                            tCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAgaga                                                                                                             >  5:274528/1‑62 (MQ=255)
                                                                                                                            tCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCg                       <  3:93641/148‑1 (MQ=255)
                                                                                                                                     cTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCTGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGccc             <  4:125761/149‑1 (MQ=255)
                                                                                                                                      ttGGAGGGGCTGCTTTTCAGCCCATTCGCGGGCAAGGCCTCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATgcgc               <  4:196864/146‑1 (MQ=255)
                                                                                                                                      ttGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGt                              >  1:361894/1‑131 (MQ=255)
                                                                                                                                      ttGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGt                              <  2:361894/131‑1 (MQ=255)
                                                                                                                                      ttGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATgcgc               >  3:196864/1‑146 (MQ=255)
                                                                                                                                         aatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCgg                      >  1:347656/4‑136 (MQ=255)
                                                                                                                                         aatgggCTGCAAAGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCgg                      <  2:347656/133‑1 (MQ=255)
                                                                                                                                                  ctTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGCAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGccc  <  7:317345/147‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGGTTTCGGTAGGGCGCGCGCACCTGGCCGACCCGCACTGGGCTTACTTTGCTGCCAAGGAGCTGGGGGTGGAAAAAGCCTCCTGGACCTTGCCGGCGCCTTATGCACACTGGCTCGAGCGTTATCGCTGAGGCTTGGAGGGGCTGCTTTGCAGCCCATTCGCGGGCAAGCCCGCACCCACAGAGACACCGCAGGTTTCAGATACTGCACCCTCTCTGTGGAAGTGGGCTTGCCCGCGAATGGGCCGGTAGGGGCTAAAACCGGTTCAGTCGGTATGCGCCCATCTCAGGCCC  >  NC_002947/1432882‑1433174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑