Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	146,127	T→A	13.2%	intergenic (+32/‑116)	gltP → / → PP_0138	glutamate/aspartate‑proton DAACS transporter/membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	146,127	0	T	A	13.2%	135.4 / 9.7	52	intergenic (+32/‑116)	gltP/PP_0138	glutamate/aspartate‑proton DAACS transporter/membrane protein
Reads supporting (aligned to +/- strand):  ref base T (23/22);  new base A (3/4);  total (26/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCG  >  NC_002947/145981‑146264                                                                                                                                                   |                                                                                                                                                   ggTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACtt                                                                                                                                                   <  1:128836/148‑1 (MQ=255)     aTTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑Cgg                                                                                                                                             >  4:210219/1‑149 (MQ=255)     aTTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑Cgg                                                                                                                                             >  4:181284/1‑149 (MQ=255)         cTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCt                                                                                                                                         >  6:165961/1‑149 (MQ=255)          tCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGAAGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCtt                                                                                                                                        >  3:108530/1‑149 (MQ=255)            gCTGGGCAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGTAGGGAGCTGTAGTGGTTGGCGAACCCGCCAAGCGCTGAGCCTGATGGCTGGTTGACCAAAAGCCCCGACAAGT‑CGGGGCtct                                                                                                                                       <  7:298137/148‑3 (MQ=255)            gCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCtttt                                                                                                                                      >  4:117664/1‑149 (MQ=255)             ccgggAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGT‑CGGGGCtcttg                                                                                                                                     <  2:120020/147‑2 (MQ=255)             cTGGCAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGGTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCttttt                                                                                                                                     <  1:298978/149‑1 (MQ=255)                  aaGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑Cgggg                                                                                                                                           >  3:111804/1‑138 (MQ=255)                  aaGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑Cgggg                                                                                                                                           <  4:111804/138‑1 (MQ=255)                      cATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGc                                                                                                                            >  6:54402/1‑149 (MQ=255)                      cATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGc                                                                                                                            <  3:148726/149‑1 (MQ=255)                       aTGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGT‑CGGGGCtctt                                                                                                                                      >  5:296707/1‑138 (MQ=255)                       aTGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACAAGT‑CGGGGCtctt                                                                                                                                      <  6:296707/138‑1 (MQ=255)                             gacgCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCt                                                                                                                     <  5:54402/149‑1 (MQ=255)                                gcgGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGa                                                                                                                  <  3:181284/149‑1 (MQ=255)                                        gggCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑cc                                                                                                    <  3:117664/149‑1 (MQ=255)                                             aGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑Caa                                                                                             <  3:210219/149‑1 (MQ=255)                                                aGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAgg                                                                                          >  1:248861/1‑149 (MQ=255)                                                      acGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGTCCGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGc                                                                                         <  1:79933/145‑1 (MQ=255)                                                      acGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGc                                                                                         >  2:79933/1‑144 (MQ=255)                                                       cGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAg                                                                                                                 >  4:343858/1‑127 (MQ=255)                                                       cGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAg                                                                                                                 <  3:343858/127‑1 (MQ=255)                                                                      cGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTca                                                                         <  1:262900/144‑1 (MQ=255)                                                                      cGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTca                                                                         >  2:262900/1‑144 (MQ=255)                                                                      cGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGc                                                                    >  4:326729/1‑149 (MQ=255)                                                                       gagaAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGACACAGGCt                                                                   >  8:160204/1‑149 (MQ=255)                                                                                  aGCTGTAGTGGTTGGCGAGACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTc                                                                                       <  5:88610/118‑1 (MQ=255)                                                                                  aGCTGTAGTGGTTGGCGAGACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTc                                                                                       >  6:88610/1‑118 (MQ=255)                                                                                      gTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTcac                                                                        <  2:4286/129‑1 (MQ=255)                                                                                       tAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTattatt                                                   >  7:307213/1‑149 (MQ=255)                                                                                          tGGTTGGCGAAACCGCCAAGCGCTTAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCg                                                 <  8:51250/148‑1 (MQ=255)                                                                                            gTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCggg                                               <  2:115244/148‑1 (MQ=255)                                                                                            gTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTCCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCggg                                               >  1:115244/1‑148 (MQ=255)                                                                                            gTTGGCGAAACCGCCAAGCGCTGAGCCTGAAGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCtgg                                               <  4:108530/148‑1 (MQ=255)                                                                                             ttGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCa                                             <  3:326729/149‑1 (MQ=255)                                                                                                  gAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATGGCCAGTCACAGGCTTCGATACGCTATTATTCGTGCACtttt                                        <  6:180439/149‑1 (MQ=255)                                                                                                   gtttcGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACtttt                                        >  5:17365/5‑148 (MQ=255)                                                                                                   aaaCCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTc                                       <  7:334456/149‑1 (MQ=255)                                                                                                         ccAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAGTAAAAAGGGATGCTGAGAAAAAGCTCGCGATCGCCAGTCACAGGCTGCGATAAGCTATTATTAGGGCACttt                                         >  1:297729/1‑149 (MQ=255)                                                                                                             gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGc                                              >  4:36088/1‑132 (MQ=255)                                                                                                             gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGc                                              <  3:36088/132‑1 (MQ=255)                                                                                                             gcgcTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACa                             <  5:165961/149‑1 (MQ=255)                                                                                                                    gCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACg                         >  4:116143/1‑146 (MQ=255)                                                                                                                    gCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACg                         <  3:116143/146‑1 (MQ=255)                                                                                                                         aTGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTc                  >  3:213938/1‑148 (MQ=255)                                                                                                                                tttGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGGG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTAACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCt          >  4:202163/1‑149 (MQ=255)                                                                                                                                      aaaaagCCCCGACAAGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTcac                                                                        >  3:259341/5‑81 (MQ=38)                                                                                                                                      aaaaagCCCCGACAAGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTcac                                                                        <  4:259341/77‑1 (MQ=38)                                                                                                                                      aaaaagCCCCGACAAGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCt    >  2:353370/5‑149 (MQ=255)                                                                                                                                        agagCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCg  >  2:79711/1‑149 (MQ=255)                                                                                                                                        agagCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCg  <  4:213938/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   GGTCATTGCTCGCTGGGAAGGCATGTACGACGCGGTCAAGGGCGAGCAGTACTACGCCTCGCTGATGGCCGAGAAGCAGGGAGCTGTAGTGGTTGGCGAAACCGCCAAGCGCTGAGCCTGATGGCTGTTTGACCAAGAGCCCCGACTTGT‑CGGGGCTTTTTGTTATCTGCGAGATCTTGAG‑‑‑‑CCGCG‑‑CCT‑A‑CAAAGGCTCGCGATCGCCAGTCACAGGCTGCGATACGCTATTATTCGGGCACTTTTCAGGGGGAACATACGGATGCTCAACGGCCTTTGGCTCG  >  NC_002947/145981‑146264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑