Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,436,436 T→C 6.6% T210A (ACC→GCC)  PP_1257 ← 1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,436,4360TC 6.6% 225.6 / 4.3 78T210A (ACC→GCC) PP_12571‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
Reads supporting (aligned to +/- strand):  ref base T (34/38);  new base C (3/2);  total (37/41)
Fisher's exact test for biased strand distribution p-value = 6.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

TCGCAGCCTTCATCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCTTGAAGCCGAT  >  NC_002947/1436288‑1436583
                                                                                                                                                    |                                                                                                                                                   
tCGCAGCCTTCATCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGt                                                                                                                                                     <  6:134370/149‑1 (MQ=255)
      ccTTCATCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGgc                                                                                                                                               <  5:106777/149‑1 (MQ=255)
         tCATCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGcc                                                                                                                                            >  6:316209/1‑149 (MQ=255)
           aTCGAACGACGACAGCACCTCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACATCGCGCGGCAGCGCATTGACGATGCCGGTGATGTCGACgg                                                                                                                                          >  4:307252/1‑149 (MQ=255)
            tCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGt                                                                                                                                         <  5:247898/149‑1 (MQ=255)
              gAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTg                                                                                                                                        <  7:246335/148‑1 (MQ=255)
              gAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTg                                                                                                                                        <  5:66293/148‑1 (MQ=255)
               aaCGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGc                                                                                                                                      <  6:31335/149‑1 (MQ=255)
                acgacgACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCg                                                                                                                                     <  4:329362/149‑1 (MQ=255)
                    cgacAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTg                                                                                                                                  >  3:123635/1‑148 (MQ=255)
                     gacAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCa                                                                                                                                <  3:46264/149‑1 (MQ=255)
                        aGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGtt                                                                                                                             <  4:63983/149‑1 (MQ=255)
                        aGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGtt                                                                                                                             >  3:63983/1‑149 (MQ=255)
                             cGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACg                                                                                                                         >  5:368625/1‑148 (MQ=255)
                              gCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACg                                                                                                                         >  3:274563/1‑147 (MQ=255)
                                 aGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGcc                                                                                                                    <  4:123635/149‑1 (MQ=255)
                                    gccgcTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATg                                                                                                                 <  7:85371/149‑1 (MQ=255)
                                      cgcTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGca                                                                                                               >  4:356978/1‑149 (MQ=255)
                                           ccAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGa                                                                                                                           <  7:79725/132‑1 (MQ=255)
                                           ccAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACAAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGa                                                                                                                           >  8:79725/1‑132 (MQ=255)
                                            cAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGGTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCt                                                                                                         >  2:314214/1‑149 (MQ=255)
                                             aGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGACGATGCCGGCGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTg                                                                                                        >  3:212750/1‑149 (MQ=255)
                                                   cgcgcCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGTTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTc                                                                                                  <  3:307252/149‑1 (MQ=255)
                                                   cgcgcCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTc                                                                                                  <  4:358692/149‑1 (MQ=255)
                                                    gcgcCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCg                                                                                                                                           <  5:227137/107‑1 (MQ=255)
                                                    gcgcCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCg                                                                                                                                           >  6:227137/1‑107 (MQ=255)
                                                        cAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGacac                                                                                             >  1:178139/1‑149 (MQ=255)
                                                        cAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGacac                                                                                             >  2:192010/1‑149 (MQ=255)
                                                          ggcggcGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCg                                                                                           >  1:242844/1‑149 (MQ=255)
                                                                 ggCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCa                                                                                    <  1:192010/149‑1 (MQ=255)
                                                                  gCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCAt                                                                                      >  2:38520/1‑146 (MQ=255)
                                                                  gCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCa                                                                                    <  1:38520/148‑1 (MQ=255)
                                                                   cACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAgg                                                                                  >  1:225430/1‑149 (MQ=255)
                                                                     cGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGt                                                                                <  5:316209/149‑1 (MQ=255)
                                                                     cGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGt                                                                                <  2:225430/149‑1 (MQ=255)
                                                                      gggCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGt                                                                                >  2:117051/1‑148 (MQ=255)
                                                                        gCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGt                                                                                                                                    >  7:207469/1‑94 (MQ=255)
                                                                        gCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGt                                                                                                                                    <  8:207469/94‑1 (MQ=255)
                                                                        gCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGAACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGa                                                                              >  2:50983/1‑148 (MQ=255)
                                                                        gCGTCGCCGTTGGCGGGCTGTTTGCTGAGGCTCACCTGTTGCAGCACTTCGCGCGGGAGCGCATTGCCTATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCAACTGGGTGTCGACTCCGACCATCAGGGTGAc                                                                             <  4:250102/149‑1 (MQ=38)
                                                                            cGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGgtgt                                                                                                   <  3:325031/123‑1 (MQ=255)
                                                                            cGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGgtgt                                                                                                   >  4:325031/1‑123 (MQ=255)
                                                                            cGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCg                                                                         <  3:224239/149‑1 (MQ=255)
                                                                              ccTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGATGAcgatgt                                                                        >  6:300228/1‑143 (MQ=255)
                                                                              ccTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGGTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTc                                                                       <  1:314214/149‑1 (MQ=255)
                                                                                    gcggcCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGa                                                                                                >  7:269033/1‑118 (MQ=255)
                                                                                    gcggcCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGa                                                                                                <  8:269033/118‑1 (MQ=255)
                                                                                         cTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAgg                                                            >  4:231623/1‑149 (MQ=255)
                                                                                          tGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGt                                                           >  5:280317/1‑149 (MQ=255)
                                                                                           gTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGt                                                           >  8:60964/1‑148 (MQ=255)
                                                                                            tttGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGt                                                           <  4:274563/147‑1 (MQ=255)
                                                                                            tttGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTg                                                          <  1:117051/148‑1 (MQ=255)
                                                                                                 tCAGGCTCACCAGTTGCAGCACTTCGCGTGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGc                                                    >  6:295613/1‑149 (MQ=255)
                                                                                                   aGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTCGCGTTCCAGTTGACGAAGCCATGCACCACATGGGTGTCGACACCGACCATCAGGGTGACATCGTCAACATTGGAGGTGATGTGCTc                                                  >  5:289541/1‑149 (MQ=39)
                                                                                                       tCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTcggcg                                              >  7:26681/1‑149 (MQ=255)
                                                                                                        cACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTcggcgg                                             >  5:259011/1‑149 (MQ=255)
                                                                                                         aCCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCggcggc                                            <  7:60964/149‑1 (MQ=255)
                                                                                                             gTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTcg                                                 <  7:80687/140‑1 (MQ=255)
                                                                                                             gTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTcg                                                 >  8:80687/1‑140 (MQ=255)
                                                                                                             gTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAg                                        >  4:144857/1‑149 (MQ=255)
                                                                                                              ttGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAgc                                       >  1:226949/1‑149 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCaa                                   <  8:135795/149‑1 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCaa                                   <  3:231623/149‑1 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGCGATGGCGCCGGTGGCGTTGCTGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATcc                                                                   <  1:26747/117‑1 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGCGATGGCGCCGGTGGCGTTGCTGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATcc                                                                   >  2:32115/1‑117 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGCGATGGCGCCGGTGGCGTTGCTGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATcc                                                                   <  1:32115/117‑1 (MQ=255)
                                                                                                                  agcaCTTCGCGCGGCAGCGCATTGCCGATGCCGGCGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATcc                                                                   >  2:26747/1‑117 (MQ=255)
                                                                                                                         cgcgggGCAGCTCATTGCCGATGCCGTTGATGGCGCAGGTGGCGTTGCAGTTGACTAAGCCATGCACCACCTGGGTGTCGACACCTACGATCAGGTTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCgtc                             <  6:289541/148‑3 (MQ=255)
                                                                                                                            gcgGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCa                         >  8:285037/1‑149 (MQ=255)
                                                                                                                                          ccGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCtt           <  5:295613/149‑1 (MQ=255)
                                                                                                                                          ccGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCtt           <  3:144857/149‑1 (MQ=255)
                                                                                                                                          ccGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCtt           <  8:26681/149‑1 (MQ=255)
                                                                                                                                          ccGATGCCGGAGATGGCGGCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGAGATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACAGCCAAACTCCtt           <  4:212750/149‑1 (MQ=255)
                                                                                                                                           cGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCaa                                   <  6:176680/124‑1 (MQ=255)
                                                                                                                                           cGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCaa                                   >  5:176680/1‑124 (MQ=255)
                                                                                                                                             aTGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCTTGaa        <  3:356978/149‑1 (MQ=255)
                                                                                                                                              tGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGTGTGACATCGTCATCCTTGGAGGTGATGTTCTCTGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCTTGAAg       <  1:50983/149‑1 (MQ=255)
                                                                                                                                                  ggTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACAACCTGGGTGTCCGCACCGACCATCAGGGAGACATCGTCTTCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCAACCGCCAAACTCCTTGAAGCCg    >  5:253333/1‑148 (MQ=38)
                                                                                                                                                   gTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCTTGAAGCCGAt  <  3:307475/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCGCAGCCTTCATCGAACGACGACAGCACCGCCAGCGCCGCTTCCAGCTCGCGCGCCAGGCGGCGGGCACGGGCGTCGCCTTTGGCGGCCTGTTTGCTCAGGCTCACCAGTTGCAGCACTTCGCGCGGCAGCGCATTGCCGATGCCGGTGATGGCGCCGGTGGCGTTGCAGTTGACGAAGCCATGCACCACCTGGGTGTCGACACCGACCATCAGGGTGACATCGTCATCCTTGGAGGTGATGTGCTCGGCGGCGTAGCGCAAGTCGGCGCCACCGCCAAACTCCTTGAAGCCGAT  >  NC_002947/1436288‑1436583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑