Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,471,553	G→C	5.5%	P425R (CCG→CGG)	alg8 ←	glycosyltransferase Alg8

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,471,553	0	G	C	5.5%	227.0 / 2.2	71	P425R (CCG→CGG)	alg8	glycosyltransferase Alg8
Reads supporting (aligned to +/- strand):  ref base G (29/38);  new base C (2/2);  total (31/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGCTGGTCCACA  >  NC_002947/1471407‑1471700                                                                                                                                                   |                                                                                                                                                    tgtTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCggg                                                                                                                                                   <  6:344825/149‑1 (MQ=255)     gAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTa                                                                                                                                               >  4:205690/1‑149 (MQ=255)       aCCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACg                                                                                                                                             >  6:47201/1‑149 (MQ=255)         cATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGtt                                                                                                                                           >  6:197374/1‑149 (MQ=255)          tttgttgAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTtg                                                                                                                                          >  2:56645/2‑149 (MQ=255)              ttgAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGc                                                                                                                                      >  1:100251/1‑149 (MQ=255)                 aaaGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATTAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCg                                                                                                                                    <  8:214405/148‑1 (MQ=255)                 aaaGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCg                                                                                                                                    >  4:48856/1‑148 (MQ=255)                  aaGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGa                                                                                                                                  <  2:203704/149‑1 (MQ=255)                   aGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAg                                                                                                                                 <  7:145830/149‑1 (MQ=255)                         cGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCAcagc                                                                                                                           <  6:29517/149‑1 (MQ=255)                         cGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCAcagc                                                                                                                           <  6:24245/149‑1 (MQ=255)                               cACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCat                                                                                                                      >  7:338619/1‑148 (MQ=255)                                aCGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCatga                                                                                                                    <  5:197374/149‑1 (MQ=255)                                aCGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCatga                                                                                                                    >  2:316500/1‑149 (MQ=255)                                aCGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGAGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCatga                                                                                                                    >  3:122817/1‑149 (MQ=255)                                      gAGGGCAGTGGGCTGAAGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTg                                                                                                               <  8:320956/148‑1 (MQ=255)                                        gggCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAg                                                                                                             <  5:47201/148‑1 (MQ=255)                                                   tGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCggg                                                                                                  >  6:162/1‑148 (MQ=255)                                                   tGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCggg                                                                                                  >  5:350805/1‑148 (MQ=255)                                                   tGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCggg                                                                                                  >  4:3800/1‑148 (MQ=255)                                                     aCGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTaa                                                                                               >  7:71097/1‑149 (MQ=255)                                                       gctgccACGAGTGCTTGTCGATGCGGAAGAACACGTAGATCTTCATCAGCGGTCCGTTGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCAGGATGGTGAGGATCAGGCGGGTAATg                                                                                             <  4:87368/145‑1 (MQ=255)                                                       gcgTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCGGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGa                                                                                                              <  2:40641/132‑1 (MQ=255)                                                            cACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGAt                                                                                        <  5:162/149‑1 (MQ=255)                                                            cACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGAt                                                                                        <  4:122817/149‑1 (MQ=255)                                                             aCGACTGCTTGTCGAGTAGGAATAACACGTAGATCTTCATCAGCGCTACGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGTCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGaac                                                                                       <  1:80430/149‑3 (MQ=255)                                                             aCGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCgg                                                                                                   <  7:307141/137‑1 (MQ=255)                                                             aCGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCgg                                                                                                   >  8:307141/1‑137 (MQ=255)                                                             aCGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATc                                                                                       >  4:333317/1‑149 (MQ=255)                                                             aCGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATc                                                                                       >  6:318399/1‑149 (MQ=255)                                                                 cTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCcaca                                                                                   <  5:303130/149‑1 (MQ=255)                                                                 cTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCca                                                                                     >  8:26105/1‑147 (MQ=255)                                                                  tGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCAcag                                                                                  >  5:335164/1‑149 (MQ=255)                                                                    cTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGa                                                                                                              <  6:72253/119‑1 (MQ=255)                                                                    cTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGa                                                                                                              >  5:72253/1‑119 (MQ=255)                                                                    cTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCAcagca                                                                                <  3:333317/149‑1 (MQ=255)                                                                    cTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCAcag                                                                                  <  1:316500/147‑1 (MQ=255)                                                                       gTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCAcagcag                                                                               <  6:350805/147‑1 (MQ=255)                                                                        tCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGa                                                                                         <  2:274600/136‑1 (MQ=255)                                                                        tCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGa                                                                                         >  1:274600/1‑136 (MQ=255)                                                                         cGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGTGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGTCGGGTAATGCCGATCCACAGCAGGTa                                                                            <  5:280403/148‑1 (MQ=255)                                                                         cGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTa                                                                            >  3:162937/1‑148 (MQ=255)                                                                          gAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTaca                                                                          >  1:152178/1‑149 (MQ=255)                                                                           aGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTacac                                                                         <  3:48856/149‑1 (MQ=255)                                                                                      cacGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAgg                                                              <  8:338619/149‑1 (MQ=255)                                                                                       ctgaaggtCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAgg                                                              <  8:70193/141‑1 (MQ=255)                                                                                                  catcaGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAAc                                                   <  3:205690/148‑1 (MQ=255)                                                                                                      aGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGa                                               <  8:71097/148‑1 (MQ=255)                                                                                                        cgcgcCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGc                                            <  6:335164/149‑1 (MQ=255)                                                                                                            ccGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGc                                        >  6:321683/1‑149 (MQ=255)                                                                                                             cGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGc                                        >  6:36933/1‑148 (MQ=255)                                                                                                             cGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGCACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGc                                        >  5:113268/1‑148 (MQ=255)                                                                                                              gatgatCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGCACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGa                                      <  6:113268/149‑1 (MQ=255)                                                                                                                    cTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCg                                >  7:158544/1‑149 (MQ=255)                                                                                                                          gTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACgg                          >  2:46506/1‑149 (MQ=255)                                                                                                                           tAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGt                         <  5:318399/149‑1 (MQ=255)                                                                                                                                gagaATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGc                    <  4:162937/149‑1 (MQ=255)                                                                                                                                 agaATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACAcc                                                                        <  3:287604/96‑1 (MQ=255)                                                                                                                                 agaATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACAcc                                                                        >  4:287604/1‑96 (MQ=255)                                                                                                                                   aaTCAGCGGGTAGGGCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATTGTGAGGATCAGGCGTGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGccc                  <  3:247961/148‑1 (MQ=255)                                                                                                                                   aaTCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCca                 <  1:46506/149‑1 (MQ=255)                                                                                                                                      cacaacgTAGGCCCGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCca                 >  5:69063/7‑146 (MQ=255)                                                                                                                                      cacaacgTAGGCCCGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCca                 <  6:69063/140‑1 (MQ=255)                                                                                                                                       acaacgTAGGCCCGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGaa                                                    >  8:141618/6‑110 (MQ=255)                                                                                                                                       acaacgTAGGCCCGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGaa                                                    <  7:141618/105‑1 (MQ=255)                                                                                                                                         cGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGc           <  1:56645/149‑1 (MQ=255)                                                                                                                                            gTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGCTgg        <  2:350877/149‑1 (MQ=255)                                                                                                                                             tAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGCTGGt       <  3:3800/149‑1 (MQ=255)                                                                                                                                                gCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACg                           <  6:236745/126‑1 (MQ=255)                                                                                                                                                gCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACg                           >  5:236745/1‑126 (MQ=255)                                                                                                                                                  cGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGCTGGTCcaca  <  5:36933/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    TGTTGAACCATTGTTGAAAGCTGGCGAGGTCACGCTTGAGGGCAGTGGGCTGACGCGTCCACGACTGCTTGTCGAGGCGGAAGAACACGTAGATCTTCATCAGCGCGCCGATGATCTGGTTGTAATAGAGAATCAGCGGGTAGGCCGGGCCTACGTTGTGGCCGGAGCACAGCAGCATGATGGTGAGGATCAGGCGGGTAATGCCGATCCACAGCAGGTACACCAGCAAGAAGGCCATGCCGAACTTGAGGCTGGCGATTACCGCCACGGTCAGGCCCAGCAGGCTGGTCCACA  >  NC_002947/1471407‑1471700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑