Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,477,214 C→T 7.8% intergenic (+149/‑133) PP_1290 → / → PP_1291 polysaccharide deacetylase family protein/PhoH family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,477,2140CT 7.8% 145.0 / 2.2 51intergenic (+149/‑133)PP_1290/PP_1291polysaccharide deacetylase family protein/PhoH family protein
Reads supporting (aligned to +/- strand):  ref base C (26/21);  new base T (2/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

GGTATGACTTCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGAC  >  NC_002947/1477068‑1477355
                                                                                                                                                    |                                                                                                                                             
ggTATGACTTCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTgca‑gca                                                                                                                                              >  1:26682/1‑148 (MQ=255)
 gTATGACTTCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑Gcaca                                                                                                                                            <  2:183054/149‑1 (MQ=255)
        ttCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCg                                                                                                                                     >  6:203733/1‑149 (MQ=255)
        ttCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCg                                                                                                                                     <  3:324252/149‑1 (MQ=255)
        ttCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAAATTAAAAAAGCCGTAAATTTGGTTTTTACGGACATGGTTTTGGC‑GGAATCAAGAAACAACACAGCCCATGCC‑GCAGGCACAGTTGGCg                                                                                                                                     >  8:341424/1‑149 (MQ=255)
        ttCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCGTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCg                                                                                                                                     >  4:74388/1‑149 (MQ=255)
             aGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATc                                                                                                                                >  5:151625/1‑149 (MQ=255)
                 tattCCCCTCTGACGCTTGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAACTGCTTTTTTCGGTCATGGTTTTTGCAGGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAg                                                                                                                             <  1:94147/146‑1 (MQ=255)
                      cccTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCAc                                                                                                                       >  3:100988/1‑149 (MQ=255)
                         tctGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCAcc                                                                                                                      >  1:362777/1‑147 (MQ=255)
                           tGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGc                                                                                                                  >  8:48843/1‑149 (MQ=255)
                             aCGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGc                                                                                                                  >  5:185640/1‑147 (MQ=255)
                              acccggCGCCAATGCCTCTTCCGTAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTAGGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAg                                                                                                               <  7:341424/145‑1 (MQ=255)
                                cTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGt                                                                                                             >  6:314161/1‑149 (MQ=255)
                                     gcCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCt                                                                                                        <  1:99725/149‑1 (MQ=255)
                                       cAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTc                                                                                                      >  5:76567/1‑149 (MQ=255)
                                             ctctTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTcccgccc                                                                                                <  6:76567/149‑1 (MQ=255)
                                                   gTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATc                                                                                                                                >  3:93696/1‑111 (MQ=255)
                                                   gTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATc                                                                                                                                <  4:93696/111‑1 (MQ=255)
                                                   gTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTcc                                                                                                     <  6:330394/138‑1 (MQ=255)
                                                   gTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTcc                                                                                                     >  5:330394/1‑138 (MQ=255)
                                                       aTCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAAcgc                                                                                      <  3:74388/149‑1 (MQ=255)
                                                       aTCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAAcgc                                                                                      <  2:362777/149‑1 (MQ=255)
                                                               aaTAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACgg                                                                              <  7:48843/149‑1 (MQ=255)
                                                               aaTAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACgg                                                                              <  5:203733/149‑1 (MQ=255)
                                                               aaTAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACgg                                                                              <  4:100988/149‑1 (MQ=255)
                                                                aTAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACggg                                                                             <  6:79306/149‑1 (MQ=255)
                                                                      ttCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GTACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGc                                                                       >  3:252101/1‑149 (MQ=255)
                                                                      ttCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GTACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGc                                                                       >  3:247264/1‑149 (MQ=255)
                                                                      ttCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGA‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCACGCCCGTAACAACGCGGATACGGGGACAGc                                                                       >  2:295291/1‑149 (MQ=255)
                                                                          aaaaTGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GTACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCa                                                                   <  4:252101/149‑1 (MQ=255)
                                                                          aaaaTGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GTACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCa                                                                   <  4:247264/149‑1 (MQ=255)
                                                                           aaaTGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAg                                                                                                                                           >  5:108807/1‑76 (MQ=255)
                                                                           aaaTGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAg                                                                                                                                           <  6:108807/76‑1 (MQ=255)
                                                                           aaaTGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAg                                                                  >  4:317318/1‑149 (MQ=255)
                                                                                  tAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCActct                                                           >  6:70091/1‑149 (MQ=255)
                                                                                                   gTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCAc                                          <  6:185640/149‑1 (MQ=255)
                                                                                                      aTGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGCTTCTCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACAACGcc                                       >  8:309566/1‑149 (MQ=255)
                                                                                                                     aTGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGCCa                         >  3:72181/1‑149 (MQ=255)
                                                                                                                      tGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACa                         >  7:204869/1‑148 (MQ=255)
                                                                                                                      tGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACa                         >  8:24643/1‑148 (MQ=255)
                                                                                                                        aagaaACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACAt                        >  8:344561/1‑147 (MQ=255)
                                                                                                                        aagaaACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATa                       <  8:204869/148‑1 (MQ=255)
                                                                                                                          gaaACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCgtgt                                   >  4:257899/1‑134 (MQ=255)
                                                                                                                          gaaACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCgtgt                                   <  3:257899/134‑1 (MQ=255)
                                                                                                                          gaaACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAgg                    <  3:317318/149‑1 (MQ=255)
                                                                                                                              ccAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGAc                <  7:24643/149‑1 (MQ=255)
                                                                                                                              ccAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGAAACGGGGACAGCGGCAGTCACTCTGCGTCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGAc                <  7:309566/149‑1 (MQ=255)
                                                                                                                                      ccGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATg        >  8:303973/1‑149 (MQ=255)
                                                                                                                                        gATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGt                   <  3:168894/136‑1 (MQ=255)
                                                                                                                                        gATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGt                   >  4:168894/1‑136 (MQ=255)
                                                                                                                                        gATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGa      >  3:334609/1‑149 (MQ=255)
                                                                                                                                             cTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGAc  >  2:319027/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                             
GGTATGACTTCTGAGCGTAGTCCCCTCTGACGCTGGCGCCAAGGCCTCTTCGTCAATCGGAAAAATAAACTTCAAAAATGCGTAAAAGTGCTTTTTTCGGTCATGGTTTTTGC‑GGTATGAAGAAACCAGACAGCCGATTCCTGCA‑GCACAGGTGGCGTCATCCAGGCCCACCTAGCCAGGTTCGCTTCCCGCCCGTAACAACGCGGATACGGGGACAGCGGCAGTCACTCTGCGGCGTAACAGACCACGCCGTGTGGCTTCGACATAAGGTGACCGAGTATGGATGAC  >  NC_002947/1477068‑1477355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑