Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,481,516 G→A 10.7% A74A (GCC→GCT)  rhlB ← ATP‑dependent RNA helicase RhlB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,481,5160GA10.7% 173.7 / 8.4 66A74A (GCC→GCT) rhlBATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base G (32/26);  new base A (5/2);  total (38/28)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.01e-01

CCAGGACCTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCGGCGT  >  NC_002947/1481371‑1481662
                                                                                                                                                   |                                                                                                                                                  
ccAGGACCTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGcc                                                                                                                                                 >  7:314472/1‑149 (MQ=255)
   ggACCTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCtgt                                                                                                                                              >  4:137396/1‑149 (MQ=255)
     aCCTGCGCCTGGATCGGCGTGCAGTACGGGAATCCCAGGTCGTGTATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCtgtg                                                                                                                                             <  7:346669/148‑1 (MQ=255)
       cTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGc                                                                                                                                                  <  5:79476/141‑1 (MQ=255)
       cTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGc                                                                                                                                                  >  6:79476/1‑141 (MQ=255)
        tGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggctt                                                                                                                                         >  5:134986/1‑149 (MQ=255)
                    ggCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGtt                                                                                                                              <  8:314472/148‑1 (MQ=255)
                           aggaCGGTAAGCCCAGGTCGTGGAGGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggcttg                                                                                                                                        <  8:150994/128‑1 (MQ=255)
                           aGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggcttt                                                                                                                                        >  7:150994/1‑130 (MQ=255)
                             tACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggctt                                                                                                                                         >  8:287812/1‑128 (MQ=255)
                             tACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCCAGCCTGTgg                                                                                                                                            <  8:25110/125‑1 (MQ=255)
                             tACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCCAGCCTGTgg                                                                                                                                            >  7:25110/1‑125 (MQ=255)
                                gggAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGttt                                                                                                                 <  4:231932/149‑1 (MQ=255)
                                gggAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGCGTATTGCCTTACTGCGGC‑TCGACCCCGAAGTCATCGGGCTTCCAC‑AGGCTCGACTGTGGCTTCGGCTTGCGTTcgcgacgccacct                                                                                                                 >  8:92136/1‑144 (MQ=255)
                                   aaGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggcttg                                                                                                                                        >  3:339706/1‑123 (MQ=255)
                                   aaGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTggcttg                                                                                                                                        <  4:339706/123‑1 (MQ=255)
                                      cccAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCtt                                                                                                           <  8:133217/149‑1 (MQ=255)
                                       ccAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑GGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGttt                                                                                                                 >  3:340730/1‑142 (MQ=255)
                                       ccAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑GGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGttt                                                                                                                 <  4:340730/142‑1 (MQ=255)
                                       ccAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTAGCGGAGAGGTTTGTCCTTg                                                                                                          >  3:72118/1‑149 (MQ=255)
                                            tCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCgg                                                                                                     <  2:337711/149‑1 (MQ=255)
                                                   tGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCg                                                                                              >  8:205891/1‑149 (MQ=255)
                                                    ggCGTGGATCAGCTGGTTGGACTGCTTGATGTCGTGGTAGCGGGTTTTGCCTTCCTGGGGC‑TGGACCACGAAGTGTTGGGGCTTCCACTGGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATc                                                                                               <  1:103208/148‑1 (MQ=255)
                                                      cctGCATAAGCTCGTAGGACAGCTTGAAGTCGTGGAAGCCGGTTAAGCCTACCTGAGGC‑TCGAACACTAACTCATCGCGCTTCCAC‑AGGCTGGCCAGTGGCATGGGCTAGCGTTCGCGGCGCGGTTTCTCCTAGGCAGGTTCATCgc                                                                                             >  4:295024/3‑146 (MQ=255)
                                                           aTCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTgg                                                                                      >  7:66730/1‑149 (MQ=255)
                                                                   gTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCgcgg                                                                              <  7:205891/149‑1 (MQ=255)
                                                                   gTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCgcgg                                                                              >  7:279351/1‑149 (MQ=255)
                                                                       gACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGcggcgg                                                                           >  4:155936/1‑148 (MQ=255)
                                                                       gACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCggcggc                                                                          <  6:310770/149‑1 (MQ=255)
                                                                          aGCTTGAAGTCGTGGAAGTGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAgg                                                                       >  2:137089/1‑149 (MQ=255)
                                                                          aGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAgg                                                                       <  5:231285/149‑1 (MQ=255)
                                                                            cTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGtt                                                                     >  3:210199/1‑149 (MQ=255)
                                                                               gAAGTCGTGGAAGTGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTg                                                                    <  1:137089/147‑1 (MQ=255)
                                                                                  gTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAg                                                               <  2:164334/149‑1 (MQ=255)
                                                                                     gTGGAAGCGGGTTTTGCCTTCCTGCGGCGTCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGcc                                                             >  1:204555/1‑149 (MQ=255)
                                                                                     gTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCt                                                            >  4:4271/1‑149 (MQ=255)
                                                                                       ggAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTg                                                          <  3:155936/149‑1 (MQ=255)
                                                                                         aaGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTggg                                                        >  3:155555/1‑149 (MQ=255)
                                                                                           gCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTggggg                                                      >  3:321485/1‑149 (MQ=255)
                                                                                             gggTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCg                                                    >  2:270729/1‑149 (MQ=255)
                                                                                                   tGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGtt                                               <  3:295024/148‑1 (MQ=255)
                                                                                                    gCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTg                                              <  2:204555/148‑1 (MQ=255)
                                                                                                       ttCCTGCGGC‑TCGACCACGAAGTCTGCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCAt                                          >  5:289988/1‑149 (MQ=255)
                                                                                                         ccTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCt                                                            <  8:318809/129‑1 (MQ=255)
                                                                                                         ccTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCt                                                            >  7:318809/1‑129 (MQ=255)
                                                                                                          cTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATgg                                        <  8:279351/148‑1 (MQ=255)
                                                                                                                  tCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTg                                                                                                          >  6:34543/1‑75 (MQ=255)
                                                                                                                     aCCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTAATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTggg                                                        <  6:147289/122‑1 (MQ=255)
                                                                                                                     aCCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTAATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTggg                                                        >  5:147289/1‑122 (MQ=255)
                                                                                                                       cACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGtt                           >  5:38892/1‑149 (MQ=255)
                                                                                                                        aCGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGttt                          >  3:28459/1‑149 (MQ=255)
                                                                                                                              tCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCcgc                    >  7:277221/1‑149 (MQ=255)
                                                                                                                                ttCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCcg                     <  7:274788/146‑1 (MQ=255)
                                                                                                                                ttCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCcg                     >  8:274788/1‑146 (MQ=255)
                                                                                                                                    ggCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGcgggccg              <  6:134986/149‑1 (MQ=255)
                                                                                                                                        gccAC‑AGGCCAGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTgg                                                         >  7:114036/2‑102 (MQ=255)
                                                                                                                                        gccAC‑AGGCCAGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTgg                                                         <  8:114036/101‑1 (MQ=255)
                                                                                                                                        gccAC‑AGGCCAGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCt          >  3:78607/2‑149 (MQ=255)
                                                                                                                                        gccAC‑AGGCCAGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCt          >  2:123450/2‑149 (MQ=255)
                                                                                                                                         ccAC‑AGGCCAGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCtt         >  5:150249/1‑149 (MQ=255)
                                                                                                                                          cac‑aGGCTGGCCTGTGGCTTGGGCTTGCGTTAGCGGCGCGGTTTGTCCTTGGCCGGTTGATAGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTc        >  7:90723/1‑149 (MQ=255)
                                                                                                                                            c‑aGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCg      <  2:322265/149‑1 (MQ=255)
                                                                                                                                              aGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCgg     >  1:358254/1‑149 (MQ=255)
                                                                                                                                               ggCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGACCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCGGc    >  1:259027/1‑149 (MQ=255)
                                                                                                                                                gCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCGGCg   <  4:28459/149‑1 (MQ=255)
                                                                                                                                                 cTGGGCGGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCCGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCGGCGt  <  8:90723/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGGACCTGCGCCTGGATCGGCGTGCAGTACGGGAAGCCCAGGTCGTGGATGGCGTGCATCAGCTCGTTGGACAGCTTGAAGTCGTGGAAGCGGGTTTTGCCTTCCTGCGGC‑TCGACCACGAAGTCTTCGGGCTTCCAC‑AGGCTGGCCTGTGGCTTGGGCTTGCGTTCGCGGCGCGGTTTGTCCTTGGCCGGTTGATCGGCAGGTGGCGTCGCGGCGGCAGGTTGCTCAGCCTTGGGGGCGGGGTTGGCATGGGGTGCGGCCGGTTTTGCCGCGGGCCGGGCTTCCGGCGT  >  NC_002947/1481371‑1481662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑