Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,504,030 T→G 8.2% R130R (CGT→CGG)  rpsI → 30S ribosomal protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,504,0300TG 8.2% 136.4 / 2.8 49R130R (CGT→CGG) rpsI30S ribosomal protein S9
Reads supporting (aligned to +/- strand):  ref base T (18/27);  new base G (2/2);  total (20/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

ACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGG  >  NC_002947/1503882‑1504174
                                                                                                                                                    |                                                                                                                                                
aCGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGt                                                                                                                                                  <  2:357497/149‑1 (MQ=255)
aCGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGt                                                                                                                                                  >  2:201428/1‑149 (MQ=255)
 cGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGtt                                                                                                                                                 <  3:101914/149‑1 (MQ=255)
  ggTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGtt                                                                                                                                                 >  1:96314/1‑148 (MQ=255)
          ccGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCt                                                                                                                                        >  3:202307/1‑149 (MQ=255)
             cgcTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCg                                                                                                                                     >  4:102044/1‑149 (MQ=255)
                  tGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCa                                                                                                                                  <  1:217297/147‑1 (MQ=255)
                    aTGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTc                                                                                                                               <  6:260376/148‑1 (MQ=255)
                              acgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGccc                                                                                                                    <  7:216895/149‑1 (MQ=255)
                                     ccTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCt                                                                                                             <  5:356441/149‑1 (MQ=255)
                                      cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTccaa                                                                                                            <  3:133678/149‑3 (MQ=255)
                                              cgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCgg                                                                                                    <  5:331900/149‑1 (MQ=255)
                                               gcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                                                                                                   <  7:2065/149‑1 (MQ=255)
                                               gcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                                                                                                   <  3:48076/149‑1 (MQ=255)
                                                ctctGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCGGGc                                                                                                  <  2:97223/149‑1 (MQ=255)
                                                   tGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATAc                                                                                                                                            <  6:340830/104‑1 (MQ=255)
                                                   tGCGTCGTGCTGGCTACGTCACCCGCGAAGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATAc                                                                                                                                            >  5:340830/1‑104 (MQ=255)
                                                                   cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAAc                                                                               >  1:283880/1‑149 (MQ=255)
                                                                   cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCCAAAAAAGCCCGGTTCCATGGAACCGGGCTTTTTTTTTAGCCTTGa                                                                                 <  1:201428/147‑1 (MQ=255)
                                                                    gTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAAc                                                                               >  6:224151/1‑148 (MQ=255)
                                                                     tCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACt                                                                              >  2:87919/1‑148 (MQ=255)
                                                                       aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTaa                                                                            <  1:105200/148‑1 (MQ=255)
                                                                       aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAAc                                                                           >  5:272259/1‑149 (MQ=255)
                                                                         ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAAcc                                                                          >  8:123853/1‑148 (MQ=255)
                                                                         ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCt                                                                         <  4:222966/149‑1 (MQ=255)
                                                                            cgACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTc                                                                      <  5:224151/149‑1 (MQ=255)
                                                                               cGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagc                                                                   <  3:102044/149‑1 (MQ=255)
                                                                                gCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagca                                                                  <  6:272259/149‑1 (MQ=255)
                                                                                     tGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcag                                                             >  2:253887/1‑149 (MQ=255)
                                                                                       aaGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcagt                                                            <  7:312096/148‑1 (MQ=255)
                                                                                                       aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAg                                           <  4:202307/149‑1 (MQ=255)
                                                                                                       aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAg                                           <  7:123853/149‑1 (MQ=255)
                                                                                                           ggTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACg                                        >  7:24520/1‑148 (MQ=255)
                                                                                                                  gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc                                         >  5:228936/1‑140 (MQ=255)
                                                                                                                  gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc                                         <  6:228936/140‑1 (MQ=255)
                                                                                                                    aaaGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg                               <  2:96314/148‑1 (MQ=255)
                                                                                                                      aGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCa                             >  4:279546/1‑148 (MQ=255)
                                                                                                                           gTAAGCGTCCTCAGTACTCCAAGCTTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCATTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAAATACGTAGATCAAGCAAAGCGt                       >  6:339867/1‑149 (MQ=255)
                                                                                                                           gTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCACCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGt                       <  1:253887/149‑1 (MQ=255)
                                                                                                                              aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                     <  3:279546/148‑1 (MQ=255)
                                                                                                                              aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                     <  2:283880/148‑1 (MQ=255)
                                                                                                                                       aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGccc           >  7:7626/1‑149 (MQ=255)
                                                                                                                                           gccgaaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGc       >  5:177715/5‑149 (MQ=255)
                                                                                                                                            tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                     >  2:230796/1‑134 (MQ=255)
                                                                                                                                            tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                     <  1:230796/134‑1 (MQ=255)
                                                                                                                                            ccgaaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGcc      >  2:34920/4‑149 (MQ=255)
                                                                                                                                             ccAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCACGGTTCCTTGGAACCGGGCTTTTTTTTTACCCTTGAACTAAACTGTCAGCACTCAGTGGCCGATAGTCCCAAAGACGTAGAACAAGCAAAGCGTGCGTTGTAGCACTTGCCg     >  4:283605/1‑149 (MQ=255)
                                                                                                                                              gaatcGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTAGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg     <  6:211897/144‑1 (MQ=255)
                                                                                                                                              cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGTAACCGGGCTTTTTTTTTAGCCTAGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg     <  6:216834/148‑1 (MQ=255)
                                                                                                                                              cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgg    >  8:17973/1‑149 (MQ=255)
                                                                                                                                               aaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg   <  1:34920/149‑1 (MQ=255)
                                                                                                                                                aGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg  <  6:177715/149‑1 (MQ=255)
                                                                                                                                                 gCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg  >  2:78697/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
ACGGTATCACCCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGG  >  NC_002947/1503882‑1504174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑