Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,504,036 C→A 8.3% intergenic (+3/‑260) rpsI → / → petA 30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,504,0360CA 8.3% 145.6 / 3.4 48intergenic (+3/‑260)rpsI/petA30S ribosomal protein S9/ubiquinol‑cytochrome c reductase iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base C (17/27);  new base A (2/2);  total (19/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTT  >  NC_002947/1503892‑1504184
                                                                                                                                                |                                                                                                                                                    
ccGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCt                                                                                                                                                  >  3:202307/1‑149 (MQ=255)
   cgcTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCg                                                                                                                                               >  4:102044/1‑149 (MQ=255)
        tGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCa                                                                                                                                            <  1:217297/147‑1 (MQ=255)
          aTGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTc                                                                                                                                         <  6:260376/148‑1 (MQ=255)
                    acgaAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGccc                                                                                                                              <  7:216895/149‑1 (MQ=255)
                           ccTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCt                                                                                                                       <  5:356441/149‑1 (MQ=255)
                            cTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTccaa                                                                                                                      <  3:133678/149‑3 (MQ=255)
                                    cgcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCgg                                                                                                              <  5:331900/149‑1 (MQ=255)
                                     gcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                                                                                                             <  7:2065/149‑1 (MQ=255)
                                     gcTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCggg                                                                                                             <  3:48076/149‑1 (MQ=255)
                                      ctctGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCAAGGAACCGGGc                                                                                                            <  2:97223/149‑1 (MQ=255)
                                         tGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATAc                                                                                                                                                      <  6:340830/104‑1 (MQ=255)
                                         tGCGTCGTGCTGGCTACGTCACCCGCGAAGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATAc                                                                                                                                                      >  5:340830/1‑104 (MQ=255)
                                                         cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAAc                                                                                         >  1:283880/1‑149 (MQ=255)
                                                         cGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCCAAAAAAGCCCGGTTCCATGGAACCGGGCTTTTTTTTTAGCCTTGa                                                                                           <  1:201428/147‑1 (MQ=255)
                                                          gTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAAc                                                                                         >  6:224151/1‑148 (MQ=255)
                                                           tCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACt                                                                                        >  2:87919/1‑148 (MQ=255)
                                                             aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTaa                                                                                      <  1:105200/148‑1 (MQ=255)
                                                             aCCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAAc                                                                                     >  5:272259/1‑149 (MQ=255)
                                                               ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAAcc                                                                                    >  8:123853/1‑148 (MQ=255)
                                                               ccGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCt                                                                                   <  4:222966/149‑1 (MQ=255)
                                                                  cgACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTc                                                                                <  5:224151/149‑1 (MQ=255)
                                                                     cGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagc                                                                             <  3:102044/149‑1 (MQ=255)
                                                                      gCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTcagca                                                                            <  6:272259/149‑1 (MQ=255)
                                                                           tGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcag                                                                       >  2:253887/1‑149 (MQ=255)
                                                                             aaGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGcagtcagt                                                                      <  7:312096/148‑1 (MQ=255)
                                                                                             aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAg                                                     <  4:202307/149‑1 (MQ=255)
                                                                                             aGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAg                                                     <  7:123853/149‑1 (MQ=255)
                                                                                                 ggTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACg                                                  >  7:24520/1‑148 (MQ=255)
                                                                                                        gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc                                                   >  5:228936/1‑140 (MQ=255)
                                                                                                        gTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGAc                                                   <  6:228936/140‑1 (MQ=255)
                                                                                                          aaaGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAg                                         <  2:96314/148‑1 (MQ=255)
                                                                                                            aGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCa                                       >  4:279546/1‑148 (MQ=255)
                                                                                                                 gTAAGCGTCCTCAGTACTCCAAGCTTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCATTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCAAATACGTAGATCAAGCAAAGCGt                                 >  6:339867/1‑149 (MQ=255)
                                                                                                                 gTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCACCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGt                                 <  1:253887/149‑1 (MQ=255)
                                                                                                                    aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  2:283880/148‑1 (MQ=255)
                                                                                                                    aGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  3:279546/148‑1 (MQ=255)
                                                                                                                             aGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGccc                     >  7:7626/1‑149 (MQ=255)
                                                                                                                                 gccgaaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGc                 >  5:177715/5‑149 (MQ=255)
                                                                                                                                  tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               >  2:230796/1‑134 (MQ=255)
                                                                                                                                  tCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTgg                               <  1:230796/134‑1 (MQ=255)
                                                                                                                                  ccgaaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGcc                >  2:34920/4‑149 (MQ=255)
                                                                                                                                   ccAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCACGGTTCCTTGGAACCGGGCTTTTTTTTTACCCTTGAACTAAACTGTCAGCACTCAGTGGCCGATAGTCCCAAAGACGTAGAACAAGCAAAGCGTGCGTTGTAGCACTTGCCg               >  4:283605/1‑149 (MQ=255)
                                                                                                                                    gaatcGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTAGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg               <  6:211897/144‑1 (MQ=255)
                                                                                                                                    cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGTAACCGGGCTTTTTTTTTAGCCTAGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCg               <  6:216834/148‑1 (MQ=255)
                                                                                                                                    cAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgg              >  8:17973/1‑149 (MQ=255)
                                                                                                                                     aaGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCggg             <  1:34920/149‑1 (MQ=255)
                                                                                                                                      aGCGGTATTAACGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg            <  6:177715/149‑1 (MQ=255)
                                                                                                                                       gCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCgggg            >  2:78697/1‑148 (MQ=255)
                                                                                                                                              tACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCAcc    <  8:24520/149‑1 (MQ=255)
                                                                                                                                                ccGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCtt  >  8:75264/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                    
CCGCGCTCTGATGGAATACGACGAAACCCTGCGTGGCGCTCTGCGTCGTGCTGGCTACGTCACCCGCGACGCTCGTGAAGTTGAGCGTAAGAAAGTGGGTCTGCGTAAAGCGCGTAAGCGTCCTCAGTACTCCAAGCGTTAATACCGCTTCGGCAGTCGAAAAAAGCCCGGTTCCTTGGAACCGGGCTTTTTTTTTAGCCTTGAACTAACCTGTCAGCAGTCAGTGGCCGCTTGTCGCATAGACGTAGATCAAGCAAAGCGTGGGTTGTCGCCCTTGCCGGGGTAATCACCTT  >  NC_002947/1503892‑1504184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑