| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,526,725 | A→C | 7.9% | Q304H (CAA→CAC) | ddlB → | D‑alanine‑‑D‑alanine ligase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,526,725 | 0 | A | C | 7.9% | 188.7 / 4.5 | 61 | Q304H (CAA→CAC) | ddlB | D‑alanine‑‑D‑alanine ligase B |
| Reads supporting (aligned to +/- strand): ref base A (29/27); new base C (3/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCG > NC_002947/1526578‑1526869 | cATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAAc > 6:15248/1‑149 (MQ=255) ttGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTg < 6:278045/149‑1 (MQ=255) ttGGCATCGAAGGCGGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGGACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTg < 3:318286/149‑1 (MQ=255) gAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCTCTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTgg < 1:98390/149‑1 (MQ=255) gggggCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTg < 4:4341/149‑1 (MQ=255) ggggCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTgg < 1:160825/149‑1 (MQ=255) gCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGcca < 6:121416/149‑2 (MQ=38) gACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGt < 1:136470/149‑1 (MQ=255) cGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTgg < 1:197509/149‑1 (MQ=255) cGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTgg > 6:35908/1‑149 (MQ=255) aGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGAcgc > 4:299252/1‑147 (MQ=255) aCGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGt < 6:105038/149‑1 (MQ=255) ggTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGc < 6:176346/149‑1 (MQ=255) gTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGc > 6:347934/1‑148 (MQ=255) ggCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGgcg > 5:273621/1‑149 (MQ=255) gctgctCGAATTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTtaataa < 2:195285/136‑1 (MQ=255) gctgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTtaataa > 1:195285/1‑136 (MQ=255) tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTg < 3:286324/116‑1 (MQ=255) tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTg > 4:286324/1‑116 (MQ=255) tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCCTGCCGGATGGCCTGGCCTTCAACCACCTGGTGATGCCAATCATGGCCGAAACCGTGCCGAGGCGAGGTTATTAACAATGAAAGccgc > 1:98461/1‑148 (MQ=255) ctCGAAGTCAACACCACACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga < 5:212551/147‑1 (MQ=255) aaGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATAc < 5:347934/149‑1 (MQ=255) gTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGt > 5:196390/1‑149 (MQ=255) aaCACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAgg < 8:2559/133‑1 (MQ=255) aaCACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGAG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACAATGCAAgg > 7:2559/1‑133 (MQ=255) acacCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTcagc < 5:35908/149‑1 (MQ=255) gCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCg > 6:243340/1‑146 (MQ=255) gCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCg < 5:243340/146‑1 (MQ=255) cAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGcc < 5:15248/149‑1 (MQ=255) cAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGcc > 2:180974/1‑149 (MQ=255) aTCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCg > 1:138897/1‑147 (MQ=255) aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga < 5:102453/111‑1 (MQ=255) aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga > 6:102453/1‑111 (MQ=255) aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAg > 1:361080/1‑149 (MQ=255) aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAg > 2:89051/1‑149 (MQ=255) cTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCg < 6:196390/149‑1 (MQ=255) cccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCaa < 6:90899/139‑1 (MQ=255) cccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCaa > 5:90899/1‑139 (MQ=255) ccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc > 8:198923/1‑149 (MQ=255) aTGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgt > 6:275606/1‑149 (MQ=255) tGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtg < 1:180974/149‑1 (MQ=255) tGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtg < 7:198923/149‑1 (MQ=255) ggggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtggt < 7:327960/147‑1 (MQ=255) gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGtt < 3:45778/76‑1 (MQ=255) gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGtt > 4:45778/1‑76 (MQ=255) gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtggt > 2:70392/1‑149 (MQ=255) ttcggCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc < 8:112801/129‑1 (MQ=25) ttcggCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc > 7:112801/4‑132 (MQ=25) gCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtg > 1:353256/1‑149 (MQ=255) gCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtg > 1:347667/1‑149 (MQ=255) cTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtgg < 2:361080/149‑1 (MQ=255) ccTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGc > 7:125488/1‑149 (MQ=255) ggattgccAGCACCAGTTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcga > 4:77133/7‑149 (MQ=255) gACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcgac > 5:332546/1‑149 (MQ=255) cTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcgac > 5:225516/1‑147 (MQ=255) cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCTTTACCGGCCGTAACAAGCCCGTGCCGCgtg > 8:273015/1‑120 (MQ=255) cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGccc > 1:258492/1‑149 (MQ=255) cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGAGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACGGGCCGTAGCAAGCCGGTGCCGCgtg < 7:273015/120‑1 (MQ=255) gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCgatgat < 6:95492/71‑1 (MQ=255) gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCgatgat > 5:95492/1‑71 (MQ=255) gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCg > 4:64467/1‑148 (MQ=255) | CATTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCG > NC_002947/1526578‑1526869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |