Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,526,727	T→A	6.7%	L305Q (CTG→CAG)	ddlB →	D‑alanine‑‑D‑alanine ligase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,526,727	0	T	A	6.7%	172.8 / 3.0	60	L305Q (CTG→CAG)	ddlB	D‑alanine‑‑D‑alanine ligase B
Reads supporting (aligned to +/- strand):  ref base T (29/27);  new base A (2/2);  total (31/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

TTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCG  >  NC_002947/1526580‑1526869                                                                                                                                                      |                                                                                                                                               ttGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTg                                                                                                                                               <  6:278045/149‑1 (MQ=255) ttGGCATCGAAGGCGGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGGACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTg                                                                                                                                               <  3:318286/149‑1 (MQ=255)         gAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGCTCTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTgg                                                                                                                                        <  1:98390/149‑1 (MQ=255)                gggggCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTg                                                                                                                                <  4:4341/149‑1 (MQ=255)                 ggggCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTgg                                                                                                                               <  1:160825/149‑1 (MQ=255)                    gCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGcca                                                                                                                            <  6:121416/149‑2 (MQ=38)                           gACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGt                                                                                                                     <  1:136470/149‑1 (MQ=255)                             cGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTgg                                                                                                                   >  6:35908/1‑149 (MQ=255)                             cGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTgg                                                                                                                   <  1:197509/149‑1 (MQ=255)                                     aGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGAcgc                                                                                                             >  4:299252/1‑147 (MQ=255)                                        aCGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGt                                                                                                        <  6:105038/149‑1 (MQ=255)                                                    ggTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGc                                                                                            <  6:176346/149‑1 (MQ=255)                                                     gTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGc                                                                                            >  6:347934/1‑148 (MQ=255)                                                          ggCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGgcg                                                                                      >  5:273621/1‑149 (MQ=255)                                                           gctgctCGAATTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTtaataa                                                                                                  <  2:195285/136‑1 (MQ=255)                                                           gctgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTtaataa                                                                                                  >  1:195285/1‑136 (MQ=255)                                                             tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTg                                                                                                                    >  4:286324/1‑116 (MQ=255)                                                             tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTg                                                                                                                    <  3:286324/116‑1 (MQ=255)                                                             tgctCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCCTGCCGGATGGCCTGGCCTTCAACCACCTGGTGATGCCAATCATGGCCGAAACCGTGCCGAGGCGAGGTTATTAACAATGAAAGccgc                                                                                     >  1:98461/1‑148 (MQ=255)                                                               ctCGAAGTCAACACCACACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga                                                                                   <  5:212551/147‑1 (MQ=255)                                                                   aaGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATAc                                                                             <  5:347934/149‑1 (MQ=255)                                                                     gTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGt                                                                           >  5:196390/1‑149 (MQ=255)                                                                        aaCACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAgg                                                                                        <  8:2559/133‑1 (MQ=255)                                                                        aaCACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGAG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACAATGCAAgg                                                                                        >  7:2559/1‑133 (MQ=255)                                                                         acacCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTcagc                                                                       <  5:35908/149‑1 (MQ=255)                                                                                     gCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCg                                                              >  6:243340/1‑146 (MQ=255)                                                                                     gCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCg                                                              <  5:243340/146‑1 (MQ=255)                                                                                           cAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGcc                                                     <  5:15248/149‑1 (MQ=255)                                                                                           cAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGcc                                                     >  2:180974/1‑149 (MQ=255)                                                                                              aTCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCg                                                    >  1:138897/1‑147 (MQ=255)                                                                                                   aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga                                                                                   <  5:102453/111‑1 (MQ=255)                                                                                                   aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCga                                                                                   >  6:102453/1‑111 (MQ=255)                                                                                                   aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAg                                             >  1:361080/1‑149 (MQ=255)                                                                                                   aGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAg                                             >  2:89051/1‑149 (MQ=255)                                                                                                       cTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCg                                          <  6:196390/149‑1 (MQ=255)                                                                                                             cccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCaa                                              <  6:90899/139‑1 (MQ=255)                                                                                                             cccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCaa                                              >  5:90899/1‑139 (MQ=255)                                                                                                              ccATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc                                   >  8:198923/1‑149 (MQ=255)                                                                                                                aTGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgt                                 >  6:275606/1‑149 (MQ=255)                                                                                                                 tGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtg                                <  1:180974/149‑1 (MQ=255)                                                                                                                 tGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtg                                <  7:198923/149‑1 (MQ=255)                                                                                                                   ggggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtggt                              <  7:327960/147‑1 (MQ=255)                                                                                                                   gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGtt                                                                                                       <  3:45778/76‑1 (MQ=255)                                                                                                                   gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGtt                                                                                                       >  4:45778/1‑76 (MQ=255)                                                                                                                   gcggcCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCgtggt                              >  2:70392/1‑149 (MQ=255)                                                                                                                                ttcggCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc                                   <  8:112801/129‑1 (MQ=25)                                                                                                                                ttcggCCAGGATTGCCAGCACCAGTTGCTGGAAGTCCAGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCcgc                                   >  7:112801/4‑132 (MQ=25)                                                                                                                                gCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtg                  >  1:353256/1‑149 (MQ=255)                                                                                                                                gCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtg                  >  1:347667/1‑149 (MQ=255)                                                                                                                                 cTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCtgg                 <  2:361080/149‑1 (MQ=255)                                                                                                                                     ccTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGc             >  7:125488/1‑149 (MQ=255)                                                                                                                                        ggattgccAGCACCAGTTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcga          >  4:77133/7‑149 (MQ=255)                                                                                                                                         gACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcgac         >  5:332546/1‑149 (MQ=255)                                                                                                                                           cTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCcgac         >  5:225516/1‑147 (MQ=255)                                                                                                                                               cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCTTTACCGGCCGTAACAAGCCCGTGCCGCgtg                                >  8:273015/1‑120 (MQ=255)                                                                                                                                               cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGccc   >  1:258492/1‑149 (MQ=255)                                                                                                                                               cAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGAGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACGGGCCGTAGCAAGCCGGTGCCGCgtg                                <  7:273015/120‑1 (MQ=255)                                                                                                                                                 gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCgatgat                                                                               <  6:95492/71‑1 (MQ=255)                                                                                                                                                 gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCgatgat                                                                               >  5:95492/1‑71 (MQ=255)                                                                                                                                                 gCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCg  >  4:64467/1‑148 (MQ=255)                                                                                                                                                      |                                                                                                                                               TTGGCATCGAAGGCTGGGGGCGGTTGGACGTGATGCAGGACGAGCAGGGCCGGTTCTGGCTGCTCGAAGTCAACACCGCACCAGGCATGACAGATCATAGC‑CTGGTGCCCATGGCGGCCCGTG‑CGGCTGGCCTGGACTTCCAGCAACTGGTGCTGGCAATCCTGGCCGAAAGCGTGGCGACGCGAGGTTAATAACCATGCAAGGCGCGATGATACGTCAGCAGCAACCCGTTACCGGCCGTAGCAAGCCGGTGCCGCGTGGTGCCAGCCGGCTGGTGGCCGACGAGCCCG  >  NC_002947/1526580‑1526869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑