Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,535,862 A→C 7.7% N291H (AAC→CAC)  argJ → glutamate N‑acetyltransferase/amino acid acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,535,8620AC 7.7% 155.7 / 2.7 52N291H (AAC→CAC) argJglutamate N‑acetyltransferase/amino acid acetyltransferase
Reads supporting (aligned to +/- strand):  ref base A (25/23);  new base C (2/2);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01

TGCCGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGCCTGATCGA  >  NC_002947/1535716‑1536010
                                                                                                                                                  |                                                                                                                                                    
tGCCGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTTGCCac                                                                                                                                                    >  6:16246/1‑149 (MQ=255)
 gCCGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAacc                                                                                                                                                   <  2:317472/149‑1 (MQ=255)
 gCCGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAac                                                                                                                                                    >  1:356464/1‑148 (MQ=255)
  ccGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTTGCCac                                                                                                                                                    <  5:16246/147‑1 (MQ=255)
    ggAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAaccac                                                                                                                                                 >  2:232593/1‑148 (MQ=255)
        gTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGa                                                                                                                                            <  2:356464/149‑1 (MQ=255)
           aCCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGt                                                                                                                                          <  5:225946/148‑1 (MQ=255)
                aGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTgg                                                                                                                                    <  1:232593/149‑1 (MQ=255)
                aGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGCGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTgg                                                                                                                                    <  3:102683/149‑1 (MQ=255)
                              ttttCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGcc                                                                                                                      >  2:221340/1‑149 (MQ=255)
                                  cGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAATGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTg                                                                                                                   <  8:145808/148‑1 (MQ=255)
                                          tGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTcg                                                                                                          >  2:232287/1‑149 (MQ=255)
                                           gAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTcgc                                                                                                         >  5:164839/1‑149 (MQ=255)
                                            aaaaaGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTcgcc                                                                                                        >  5:207518/1‑149 (MQ=255)
                                                  gCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGAt                                                                                                  >  8:241630/1‑149 (MQ=255)
                                                      tgtTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATc                                                                                                 >  6:169312/1‑146 (MQ=255)
                                                                      ggAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGt                                                                                                                                <  5:303134/99‑1 (MQ=255)
                                                                      ggAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGt                                                                                                                                >  6:303134/1‑99 (MQ=255)
                                                                        aaGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTcc                                                                            <  5:81580/149‑1 (MQ=255)
                                                                        aaGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTcc                                                                            >  5:2610/1‑149 (MQ=255)
                                                                        aaGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTcc                                                                            >  7:132656/1‑149 (MQ=255)
                                                                           tGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGAc                                                                         <  5:169312/149‑1 (MQ=255)
                                                                            ggCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGAc                                                                         <  1:221340/148‑1 (MQ=255)
                                                                              cccAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGAccc                                                                       >  3:270360/1‑148 (MQ=255)
                                                                              cccAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACCCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCg                                                                      >  8:21029/1‑149 (MQ=255)
                                                                               ccAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCg                                                                      >  6:292221/1‑148 (MQ=255)
                                                                                cAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTc                                                                                  <  6:6851/135‑1 (MQ=255)
                                                                                cAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTc                                                                                  >  5:6851/1‑135 (MQ=255)
                                                                                 aGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAAc                                                                   <  1:232287/149‑1 (MQ=255)
                                                                                 aGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAAc                                                                   <  1:106531/149‑1 (MQ=255)
                                                                                  ggCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACt                                                                  <  8:132656/149‑1 (MQ=255)
                                                                                   gCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTg                                                                 >  5:193042/1‑149 (MQ=255)
                                                                                   gCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTAGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTcc                                                                            <  5:344081/138‑1 (MQ=255)
                                                                                   gCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTAGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTcc                                                                            >  6:344081/1‑138 (MQ=255)
                                                                                     cATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTggg                                                               <  6:207518/149‑1 (MQ=255)
                                                                                          tCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGc                                                          <  5:108647/149‑1 (MQ=255)
                                                                                                     gAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTcgccgc                                               >  5:136137/1‑149 (MQ=255)
                                                                                                          tGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGGATCCTCGCCGCCGTAg                                          <  7:21029/149‑1 (MQ=255)
                                                                                                                   gTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCTAACTGGGGTCGCATCCTCGCCGCCGTAGGCCGTGCCg                                 <  6:193042/149‑1 (MQ=255)
                                                                                                                    ttCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCgg                                >  5:83977/1‑149 (MQ=255)
                                                                                                                             gtgcagtgcaTCGGCCGTGCCACCCACCAGGCTTGCCTGGACTGCGGCTACCCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGACCTGGGGCCGCATACTCGCCGCCGTAGGCCGTGCCGGTGTACCg                         <  4:277107/147‑1 (MQ=255)
                                                                                                                             gTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCAGGTGTACCg                         >  3:277107/1‑147 (MQ=255)
                                                                                                                              tGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAg                      >  3:31292/1‑149 (MQ=255)
                                                                                                                                     tcctggtggtTGCCACCACCGGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACt                                                                  >  6:124394/5‑98 (MQ=255)
                                                                                                                                     tcctggtggtTGCCACCACCGGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACt                                                                  <  5:124394/94‑1 (MQ=255)
                                                                                                                                      aaCGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGAtgt              >  2:269994/1‑149 (MQ=255)
                                                                                                                                        cGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGAtgt              >  5:194897/1‑147 (MQ=255)
                                                                                                                                        cGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCATCCGCCCCGAACTGCGGCCGCATCCTCGCCGCCGTACGCCGTGCCGGTGTCCCGGAGCTGGAtgt              >  8:86856/1‑147 (MQ=255)
                                                                                                                                          gtggtgGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGc          <  6:136137/149‑1 (MQ=255)
                                                                                                                                              tggCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCCCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGCCTGa      <  7:241630/149‑1 (MQ=255)
                                                                                                                                               ggCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACAAGCCACAAATAAAAACCGCGCTGTTAGCTAACGACCCGAACATGGGCCGCAAACACCCCGCCGTAGGCCGAGACGGAGTACCCGACCAGGAAGTGAGCCAGaa     >  8:228428/1‑148 (MQ=255)
                                                                                                                                                gCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGCCTGAt     <  5:292221/148‑1 (MQ=255)
                                                                                                                                                  aaCCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGTCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGCCTGATCGa  >  4:72064/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TGCCGGAAGTGACCGAAGCCAGCGGCGCGCTTTTCGAAGCGCTGAAAAAGGCCGTGTTCGAAGTGTGCATGGAAGTGGCCCAGGCCATCGTCCGTGACGGCGAAGGTGCGACCAAGTTCGTGACCGTGCAGGTCAACGGTGGTGGCAACCACCAGGAGTGCCTGGACGTCGGCTACGCCGTGGCCCACTCGCCGCTGATCAAGACCGCGCTGTTCGCTTCCGACCCGAACTGGGGCCGCATCCTCGCCGCCGTAGGCCGTGCCGGTGTACCGGAGCTGGATGTGAGCCTGATCGA  >  NC_002947/1535716‑1536010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑