| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,550,541 | T→A | 6.3% | L314Q (CTG→CAG) | groL → | chaperonin GroEL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,550,541 | 0 | T | A | 6.3% | 187.4 / 2.8 | 63 | L314Q (CTG→CAG) | groL | chaperonin GroEL |
| Reads supporting (aligned to +/- strand): ref base T (31/28); new base A (2/2); total (33/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
ACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGTGA > NC_002947/1550394‑1550688 | acaacaTGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTg < 7:32967/149‑1 (MQ=255) aaGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCAGGTGCTCCAGGGTAGCg > 4:78709/1‑145 (MQ=255) aaGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCAGGTGCTCCAGGGTAGCg < 3:78709/145‑1 (MQ=255) gTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAgcg > 1:52025/1‑149 (MQ=255) gTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGAAGCACCTGGGTAACGCCAAgcg > 3:156148/1‑149 (MQ=255) ctgcAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGt > 1:185601/1‑148 (MQ=255) gcAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGa < 5:14623/122‑1 (MQ=255) gcAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGa > 6:14623/1‑122 (MQ=255) gcAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCAt > 3:250764/1‑149 (MQ=255) tCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTg > 7:159244/1‑149 (MQ=255) aaGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTc < 4:250764/149‑1 (MQ=255) gggCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGAAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGaaa < 4:156148/149‑1 (MQ=255) ggCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGaaaa < 4:232655/149‑1 (MQ=255) gCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGaaaa > 7:303943/1‑148 (MQ=255) gCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAAcacca < 5:84219/147‑1 (MQ=255) gCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAAcacca > 6:84219/1‑147 (MQ=255) gATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGaa > 4:237740/1‑138 (MQ=255) gATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGaa < 3:237740/138‑1 (MQ=255) gccgcAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACcatcat < 2:52025/148‑1 (MQ=255) cgcAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCAGGTGCTCCAggg > 4:28780/1‑101 (MQ=255) cgcAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCAGGTGCTCCAggg < 3:28780/101‑1 (MQ=255) cATGCTGCAGGACATCTCTGTCGTGACTGGCGGCCAGGTCATCTCCGAAGATATCGGCCTGTCCGTTGAAAGCGCTAGCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGAct < 4:187965/143‑2 (MQ=255) cATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAac < 2:362865/127‑1 (MQ=255) cATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAac > 1:362865/1‑127 (MQ=255) cATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAAGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACAACACCATCATCGACGGTGCTg > 6:189823/1‑149 (MQ=255) aaTGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGCTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGAcc > 3:187965/2‑142 (MQ=255) cagtcAGGACAGCGATGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAac > 3:37948/5‑124 (MQ=255) tgcAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCt < 8:303943/149‑1 (MQ=255) cAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTGATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGa < 5:234874/149‑1 (MQ=255) aGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGa > 2:206253/1‑115 (MQ=255) gCTGTCCTGACTGGCGGCCAAGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACAGCACCATCATCGACGGTGCTGGCGCTGACACCGAGAt < 5:189823/149‑1 (MQ=255) tCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGaa < 8:333774/149‑1 (MQ=255) tGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCt > 1:317568/1‑131 (MQ=255) tGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCt < 2:317568/131‑1 (MQ=255) ggcCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAgg > 7:202169/1‑91 (MQ=255) ggcCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAgg < 8:202169/91‑1 (MQ=255) gcCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCAcggatctgc > 1:293303/1‑142 (MQ=255) gTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGa > 1:236591/1‑131 (MQ=255) gTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGa < 2:236591/131‑1 (MQ=255) aTCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCg > 1:254710/1‑149 (MQ=255) tcCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGc > 4:295609/1‑149 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCgaga < 8:270341/118‑1 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCgaga > 7:270341/1‑118 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTc > 5:199873/1‑132 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTc < 6:199873/132‑1 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGcc > 1:37540/1‑147 (MQ=255) gaagaaATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGcc > 5:328777/1‑147 (MQ=255) cGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCg > 4:303877/1‑146 (MQ=255) ggCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCgaag < 6:11157/148‑1 (MQ=255) ggCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCgaag < 6:6006/148‑1 (MQ=255) ccTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAg > 5:115344/1‑107 (MQ=255) ccTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAg < 6:115344/107‑1 (MQ=255) ccTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCt > 2:308067/1‑149 (MQ=255) cTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTc > 6:240346/1‑149 (MQ=255) tGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCg > 5:139078/1‑149 (MQ=255) aaaCCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGc > 7:162880/1‑114 (MQ=255) aaaCCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGc < 8:162880/114‑1 (MQ=255) gCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGa < 6:139078/149‑1 (MQ=255) cccTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGAcc < 5:240346/147‑1 (MQ=255) cccTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGAcc < 2:37540/147‑1 (MQ=255) ccTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGt > 3:117276/1‑148 (MQ=255) ccTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGt < 4:117276/148‑1 (MQ=255) cTGGAGCACCTGGGAAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGTga < 2:254710/149‑1 (MQ=255) tGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGt > 1:345997/1‑146 (MQ=255) | ACAACATGCGCGGCATCGTCAAGGTTGCTGCAGTCAAGGCTCCGGGCTTCGGCGATCGCCGCAAGGCCATGCTGCAGGACATCGCTGTCCTGACTGGCGGCCAGGTCATCTCCGAAGAAATCGGCCTGTCCCTGGAAACCGCTACCCTGGAGCACCTGGGTAACGCCAAGCGCGTCATCCTGTCCAAGGAAAACACCACCATCATCGACGGTGCTGGCGCTGACACCGAGATCGAAGCACGCGTCAAGCAGATCCGTGCCCAGATCGAAGAAACTTCCTCGGACTACGACCGTGA > NC_002947/1550394‑1550688 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |